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The Compound of Mangiferin-Berberine Salt Has Potent Activities in Modulating Lipid and Glucose Metabolisms in HepG2 Cells.

Wang C, Jiang JD, Wu W, Kong WJ - Biomed Res Int (2016)

Bottom Line: The above activities of MB salt were AMPK dependent and were superior to those of M or B when administered at an equal molar concentration.MB salt enhanced basal and insulin-stimulated glucose consumption and suppressed gluconeogenesis more potently than M or B alone.Our results may support MB salt as a new kind of agent for the development of novel lipid or glucose-lowering drugs in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Virology, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

ABSTRACT
The mangiferin-berberine (MB) salt was synthesized by ionic bonding of mangiferin (M) and berberine (B) at an equal molecular ratio. This study aimed to investigate the activities of MB salt in modulating lipid and glucose metabolisms in HepG2 cells. After 24 h treatment of the studying compounds, cellular AMP-activated protein kinase α (AMPKα)/acetyl-CoA carboxylase (ACC) protein levels and carnitine palmitoyltransferase (CPT) 1 activities, intracellular lipid contents, mRNA expression levels of target genes, glucose consumption, and glucose production amounts were determined. Compound C (CC) was used in the blocking experiments. Our results showed that MB salt increased p-AMPKα (Thr172)/p-ACC (Ser79) levels and CPT1 activity and suppressed oleic acid- (OA-) induced lipid accumulation and upregulation of lipogenic genes potently in HepG2 cells. The above activities of MB salt were AMPK dependent and were superior to those of M or B when administered at an equal molar concentration. MB salt enhanced basal and insulin-stimulated glucose consumption and suppressed gluconeogenesis more potently than M or B alone. The inhibiting activity of MB salt on cellular gluconeogenesis was AMPK dependent. Our results may support MB salt as a new kind of agent for the development of novel lipid or glucose-lowering drugs in the future.

No MeSH data available.


Related in: MedlinePlus

Influence of MB salt/M/B on cell viability. (a) Chemical structure and molecular weight (MW) of MB salt. (b) HepG2 cells were treated with the studying compounds at different concentrations for 24 h. Cell viability was determined by MTT staining and presented as percentages of control cells, which were treated with DMSO (0.5%) and defined as 100. Values are mean ± SD of 3 separate experiments; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus that of DMSO.
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fig1: Influence of MB salt/M/B on cell viability. (a) Chemical structure and molecular weight (MW) of MB salt. (b) HepG2 cells were treated with the studying compounds at different concentrations for 24 h. Cell viability was determined by MTT staining and presented as percentages of control cells, which were treated with DMSO (0.5%) and defined as 100. Values are mean ± SD of 3 separate experiments; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus that of DMSO.

Mentions: Mangiferin (M, Figure 1(a)), a xanthone glycoside, is a natural compound extracted from plants such as Mangifera indica and Anemarrhena asphodeloides. It was reported to have hypolipidemic [4–7], hypoglycemic [8–12], insulin-sensitizing [13], antiobesity [8, 9], antioxidative [14–16], and anti-inflammatory [14, 17] activities in animal models as well as in clinic. The beneficial effects of M on lipid and glucose metabolisms might be related to the activation of AMP-activated protein kinase (AMPK) [5, 17, 18], a key molecule that controls energy balance and metabolism in organisms [19].


The Compound of Mangiferin-Berberine Salt Has Potent Activities in Modulating Lipid and Glucose Metabolisms in HepG2 Cells.

Wang C, Jiang JD, Wu W, Kong WJ - Biomed Res Int (2016)

Influence of MB salt/M/B on cell viability. (a) Chemical structure and molecular weight (MW) of MB salt. (b) HepG2 cells were treated with the studying compounds at different concentrations for 24 h. Cell viability was determined by MTT staining and presented as percentages of control cells, which were treated with DMSO (0.5%) and defined as 100. Values are mean ± SD of 3 separate experiments; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus that of DMSO.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4829696&req=5

fig1: Influence of MB salt/M/B on cell viability. (a) Chemical structure and molecular weight (MW) of MB salt. (b) HepG2 cells were treated with the studying compounds at different concentrations for 24 h. Cell viability was determined by MTT staining and presented as percentages of control cells, which were treated with DMSO (0.5%) and defined as 100. Values are mean ± SD of 3 separate experiments; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus that of DMSO.
Mentions: Mangiferin (M, Figure 1(a)), a xanthone glycoside, is a natural compound extracted from plants such as Mangifera indica and Anemarrhena asphodeloides. It was reported to have hypolipidemic [4–7], hypoglycemic [8–12], insulin-sensitizing [13], antiobesity [8, 9], antioxidative [14–16], and anti-inflammatory [14, 17] activities in animal models as well as in clinic. The beneficial effects of M on lipid and glucose metabolisms might be related to the activation of AMP-activated protein kinase (AMPK) [5, 17, 18], a key molecule that controls energy balance and metabolism in organisms [19].

Bottom Line: The above activities of MB salt were AMPK dependent and were superior to those of M or B when administered at an equal molar concentration.MB salt enhanced basal and insulin-stimulated glucose consumption and suppressed gluconeogenesis more potently than M or B alone.Our results may support MB salt as a new kind of agent for the development of novel lipid or glucose-lowering drugs in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Virology, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

ABSTRACT
The mangiferin-berberine (MB) salt was synthesized by ionic bonding of mangiferin (M) and berberine (B) at an equal molecular ratio. This study aimed to investigate the activities of MB salt in modulating lipid and glucose metabolisms in HepG2 cells. After 24 h treatment of the studying compounds, cellular AMP-activated protein kinase α (AMPKα)/acetyl-CoA carboxylase (ACC) protein levels and carnitine palmitoyltransferase (CPT) 1 activities, intracellular lipid contents, mRNA expression levels of target genes, glucose consumption, and glucose production amounts were determined. Compound C (CC) was used in the blocking experiments. Our results showed that MB salt increased p-AMPKα (Thr172)/p-ACC (Ser79) levels and CPT1 activity and suppressed oleic acid- (OA-) induced lipid accumulation and upregulation of lipogenic genes potently in HepG2 cells. The above activities of MB salt were AMPK dependent and were superior to those of M or B when administered at an equal molar concentration. MB salt enhanced basal and insulin-stimulated glucose consumption and suppressed gluconeogenesis more potently than M or B alone. The inhibiting activity of MB salt on cellular gluconeogenesis was AMPK dependent. Our results may support MB salt as a new kind of agent for the development of novel lipid or glucose-lowering drugs in the future.

No MeSH data available.


Related in: MedlinePlus