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Paraphyletic genus Ditylenchus Filipjev (Nematoda, Tylenchida), corresponding to the D. triformis-group and the D. dipsaci-group scheme.

Qiao Y, Yu Q, Badiss A, Zaidi MA - Zookeys (2016)

Bottom Line: DNA sequences of regions of the nuclear ribosomal first internal transcribed spacer (ITS1) and the small subunit 18S were PCR amplified, sequenced and the phylogenetic analyses also including the sequences of the closely related species from the GenBank.Two clusters or clades corresponding to the 2 groups were consistently observed with significant statistical support from the 3 datasets.The phylogenetic analysis also revealed that the genus is paraphyletic, separating the 2 groups by species of Anguina and Subanguina.

View Article: PubMed Central - HTML - PubMed

Affiliation: China Agriculture University, Beijing, China.

ABSTRACT
The genus Ditylenchus has been divided into 2 groups: the Ditylenchus triformis-group, and the Ditylenchus dipsaci-group based on morphological and biological characters. A total of 18 populations belong to 5 species of Ditylenchus was studied: Ditylenchus africanus, Ditylenchus destructor, Ditylenchus myceliophagus and dipsaci, Ditylenchus weischeri, the first 3 belong to the Ditylenchus triformis-group, the last 2 the Ditylenchus dipsaci-group. The species of Ditylenchus triformis-group were cultured on fungi, while the species from Ditylenchus dispaci-group cultured on excised roots of plant hosts in petri dish. DNA sequences of regions of the nuclear ribosomal first internal transcribed spacer (ITS1) and the small subunit 18S were PCR amplified, sequenced and the phylogenetic analyses also including the sequences of the closely related species from the GenBank. The randomly amplified polymorphisms of genomic DNA (RAPD) were also generated. Two clusters or clades corresponding to the 2 groups were consistently observed with significant statistical support from the 3 datasets. The phylogenetic analysis also revealed that the genus is paraphyletic, separating the 2 groups by species of Anguina and Subanguina.

No MeSH data available.


Related in: MedlinePlus

Un-weighted Pair Group Method with Arithmetic Mean (UPGMA) tree showing estimated average genetic distances among all Ditylenchus species based on simple matching coefficient obtained from RAPD analysis.
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Figure 4: Un-weighted Pair Group Method with Arithmetic Mean (UPGMA) tree showing estimated average genetic distances among all Ditylenchus species based on simple matching coefficient obtained from RAPD analysis.

Mentions: The RAPD binary data matrix and resulting simple matching coefficient (SM) are presented in Table 2. Figure 4 shows the dendrogram indicating the relationships among all collections. Species of Ditylenchus separated into two clusters consistent with the phylogenetic results based on the ITS1 and 18S sequences. Ditylenchusdestructor, Ditylenchusafricanus, and Ditylenchusmyceliophagus comprised one cluster and Ditylenchusdipsaci and Ditylenchusweischeri the second cluster. All Ditylenchusdestructor populations were in one cluster with similarity of 74.2%, and all six populations of Ditylenchusdipsaci in the other cluster with a higher degree of genetic similarity (87%).


Paraphyletic genus Ditylenchus Filipjev (Nematoda, Tylenchida), corresponding to the D. triformis-group and the D. dipsaci-group scheme.

Qiao Y, Yu Q, Badiss A, Zaidi MA - Zookeys (2016)

Un-weighted Pair Group Method with Arithmetic Mean (UPGMA) tree showing estimated average genetic distances among all Ditylenchus species based on simple matching coefficient obtained from RAPD analysis.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829666&req=5

Figure 4: Un-weighted Pair Group Method with Arithmetic Mean (UPGMA) tree showing estimated average genetic distances among all Ditylenchus species based on simple matching coefficient obtained from RAPD analysis.
Mentions: The RAPD binary data matrix and resulting simple matching coefficient (SM) are presented in Table 2. Figure 4 shows the dendrogram indicating the relationships among all collections. Species of Ditylenchus separated into two clusters consistent with the phylogenetic results based on the ITS1 and 18S sequences. Ditylenchusdestructor, Ditylenchusafricanus, and Ditylenchusmyceliophagus comprised one cluster and Ditylenchusdipsaci and Ditylenchusweischeri the second cluster. All Ditylenchusdestructor populations were in one cluster with similarity of 74.2%, and all six populations of Ditylenchusdipsaci in the other cluster with a higher degree of genetic similarity (87%).

Bottom Line: DNA sequences of regions of the nuclear ribosomal first internal transcribed spacer (ITS1) and the small subunit 18S were PCR amplified, sequenced and the phylogenetic analyses also including the sequences of the closely related species from the GenBank.Two clusters or clades corresponding to the 2 groups were consistently observed with significant statistical support from the 3 datasets.The phylogenetic analysis also revealed that the genus is paraphyletic, separating the 2 groups by species of Anguina and Subanguina.

View Article: PubMed Central - HTML - PubMed

Affiliation: China Agriculture University, Beijing, China.

ABSTRACT
The genus Ditylenchus has been divided into 2 groups: the Ditylenchus triformis-group, and the Ditylenchus dipsaci-group based on morphological and biological characters. A total of 18 populations belong to 5 species of Ditylenchus was studied: Ditylenchus africanus, Ditylenchus destructor, Ditylenchus myceliophagus and dipsaci, Ditylenchus weischeri, the first 3 belong to the Ditylenchus triformis-group, the last 2 the Ditylenchus dipsaci-group. The species of Ditylenchus triformis-group were cultured on fungi, while the species from Ditylenchus dispaci-group cultured on excised roots of plant hosts in petri dish. DNA sequences of regions of the nuclear ribosomal first internal transcribed spacer (ITS1) and the small subunit 18S were PCR amplified, sequenced and the phylogenetic analyses also including the sequences of the closely related species from the GenBank. The randomly amplified polymorphisms of genomic DNA (RAPD) were also generated. Two clusters or clades corresponding to the 2 groups were consistently observed with significant statistical support from the 3 datasets. The phylogenetic analysis also revealed that the genus is paraphyletic, separating the 2 groups by species of Anguina and Subanguina.

No MeSH data available.


Related in: MedlinePlus