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Continuing Reassortant of H5N6 Subtype Highly Pathogenic Avian Influenza Virus in Guangdong.

Yuan R, Wang Z, Kang Y, Wu J, Zou L, Liang L, Song Y, Zhang X, Ni H, Lin J, Ke C - Front Microbiol (2016)

Bottom Line: With environmental samples associated with these LPMs, we performed genetic and phylogenetic analyses of 10 H5N6 AIVs isolated from different cities of Guangdong Province from different years.Phylogenetic analyses revealed that the environmental H5N6 AIV related closely to human H5N6 AIVs isolated in Guangdong.These results thus suggest that continued reassortment has enabled the emergence of a novel H5N6 virus in Guangdong, as well as highlight the potential risk of highly pathogenic H5N6 AIVs in the province.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Repository and Application of Pathogenic Microbiology, Research Center for Pathogens Detection Technology of Emerging Infectious Diseases, Guangdong Provincial Center for Disease Control and PreventionGuangzhou, China; WHO Collaborating Centre for Surveillance, Research and Training of Emerging Infectious DiseaseGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China.

ABSTRACT
First identified in May 2014 in China's Sichuan Province, initial cases of H5N6 avian influenza virus (AIV) infection in humans raised great concerns about the virus's prevalence, origin, and development. To evaluate both AIV contamination in live poultry markets (LPMs) and the risk of AIV infection in humans, we have conducted surveillance of LPMs in Guangdong Province since 2013 as part of environmental sampling programs. With environmental samples associated with these LPMs, we performed genetic and phylogenetic analyses of 10 H5N6 AIVs isolated from different cities of Guangdong Province from different years. Results revealed that the H5N6 viruses were reassortants with hemagglutinin (HA) genes derived from clade 2.3.4.4 of H5-subtype AIV, yet neuraminidase (NA) genes derived from H6N6 AIV. Unlike the other seven H5N6 viruses isolated in first 7 months of 2014, all of which shared remarkable sequence similarity with the H5N1 AIV in all internal genes, the PB2 genes of GZ693, GZ670, and ZS558 more closely related to H6N6 AIV and the PB1 gene of GZ693 to the H3-subtype AIV. Phylogenetic analyses revealed that the environmental H5N6 AIV related closely to human H5N6 AIVs isolated in Guangdong. These results thus suggest that continued reassortment has enabled the emergence of a novel H5N6 virus in Guangdong, as well as highlight the potential risk of highly pathogenic H5N6 AIVs in the province.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analyses of the open reading frames of H5N6-subtype avian influenza viruses. Viruses highlighted with black triangles (▴) were characterized in the present study. The tree was constructed using the neighbor-joining of Molecular Evolutionary Genetics Analysis 6.06, with 1000 bootstrap trials to ensure confidence in the groupings.
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Figure 1: Phylogenetic analyses of the open reading frames of H5N6-subtype avian influenza viruses. Viruses highlighted with black triangles (▴) were characterized in the present study. The tree was constructed using the neighbor-joining of Molecular Evolutionary Genetics Analysis 6.06, with 1000 bootstrap trials to ensure confidence in the groupings.

Mentions: Phylogenetic analyses demonstrated the origin and evolution of H5N6 AIVs in China. As results of the phylogenetic analysis of H5 and related viruses show, the HA gene of all 10 viruses clustered into clade 2.3.4.4 (Figure 1A) and thus related more closely to the H5N2 HPAIV, A/chicken/Zhejiang/727159/2014(H5N2), which circulates in Zhejiang Province (Figures 1A, 2). In addition, QY025, QY197, QY208, GZ670, and ZS558 shared 98.5–99.9% highest nucleotide similarity with A/chicken/Dongguan/2690/2013(H5N6) (GD-H5N6), JY137, PY955, and ZS356 shared 99.1–99.6% highest nucleotide similarity with A/chicken/Shenzhen/1395/2013(H5N6) (GD-H5N6), and HY243 shared 99.1% highest nucleotide similarity with JX-H5N6. More singularly, GZ693 shared 98.7% highest nucleotide similarity with A/Guangdong/ZQ874/2015(H5N6) (ZQ874), which was found to have recently infected human in Zhaoqing, Guangdong Province.


Continuing Reassortant of H5N6 Subtype Highly Pathogenic Avian Influenza Virus in Guangdong.

Yuan R, Wang Z, Kang Y, Wu J, Zou L, Liang L, Song Y, Zhang X, Ni H, Lin J, Ke C - Front Microbiol (2016)

Phylogenetic analyses of the open reading frames of H5N6-subtype avian influenza viruses. Viruses highlighted with black triangles (▴) were characterized in the present study. The tree was constructed using the neighbor-joining of Molecular Evolutionary Genetics Analysis 6.06, with 1000 bootstrap trials to ensure confidence in the groupings.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829614&req=5

Figure 1: Phylogenetic analyses of the open reading frames of H5N6-subtype avian influenza viruses. Viruses highlighted with black triangles (▴) were characterized in the present study. The tree was constructed using the neighbor-joining of Molecular Evolutionary Genetics Analysis 6.06, with 1000 bootstrap trials to ensure confidence in the groupings.
Mentions: Phylogenetic analyses demonstrated the origin and evolution of H5N6 AIVs in China. As results of the phylogenetic analysis of H5 and related viruses show, the HA gene of all 10 viruses clustered into clade 2.3.4.4 (Figure 1A) and thus related more closely to the H5N2 HPAIV, A/chicken/Zhejiang/727159/2014(H5N2), which circulates in Zhejiang Province (Figures 1A, 2). In addition, QY025, QY197, QY208, GZ670, and ZS558 shared 98.5–99.9% highest nucleotide similarity with A/chicken/Dongguan/2690/2013(H5N6) (GD-H5N6), JY137, PY955, and ZS356 shared 99.1–99.6% highest nucleotide similarity with A/chicken/Shenzhen/1395/2013(H5N6) (GD-H5N6), and HY243 shared 99.1% highest nucleotide similarity with JX-H5N6. More singularly, GZ693 shared 98.7% highest nucleotide similarity with A/Guangdong/ZQ874/2015(H5N6) (ZQ874), which was found to have recently infected human in Zhaoqing, Guangdong Province.

Bottom Line: With environmental samples associated with these LPMs, we performed genetic and phylogenetic analyses of 10 H5N6 AIVs isolated from different cities of Guangdong Province from different years.Phylogenetic analyses revealed that the environmental H5N6 AIV related closely to human H5N6 AIVs isolated in Guangdong.These results thus suggest that continued reassortment has enabled the emergence of a novel H5N6 virus in Guangdong, as well as highlight the potential risk of highly pathogenic H5N6 AIVs in the province.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Repository and Application of Pathogenic Microbiology, Research Center for Pathogens Detection Technology of Emerging Infectious Diseases, Guangdong Provincial Center for Disease Control and PreventionGuangzhou, China; WHO Collaborating Centre for Surveillance, Research and Training of Emerging Infectious DiseaseGuangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China.

ABSTRACT
First identified in May 2014 in China's Sichuan Province, initial cases of H5N6 avian influenza virus (AIV) infection in humans raised great concerns about the virus's prevalence, origin, and development. To evaluate both AIV contamination in live poultry markets (LPMs) and the risk of AIV infection in humans, we have conducted surveillance of LPMs in Guangdong Province since 2013 as part of environmental sampling programs. With environmental samples associated with these LPMs, we performed genetic and phylogenetic analyses of 10 H5N6 AIVs isolated from different cities of Guangdong Province from different years. Results revealed that the H5N6 viruses were reassortants with hemagglutinin (HA) genes derived from clade 2.3.4.4 of H5-subtype AIV, yet neuraminidase (NA) genes derived from H6N6 AIV. Unlike the other seven H5N6 viruses isolated in first 7 months of 2014, all of which shared remarkable sequence similarity with the H5N1 AIV in all internal genes, the PB2 genes of GZ693, GZ670, and ZS558 more closely related to H6N6 AIV and the PB1 gene of GZ693 to the H3-subtype AIV. Phylogenetic analyses revealed that the environmental H5N6 AIV related closely to human H5N6 AIVs isolated in Guangdong. These results thus suggest that continued reassortment has enabled the emergence of a novel H5N6 virus in Guangdong, as well as highlight the potential risk of highly pathogenic H5N6 AIVs in the province.

No MeSH data available.


Related in: MedlinePlus