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Sex Difference of Egfr Expression and Molecular Pathway in the Liver: Impact on Drug Design and Cancer Treatments?

Wang L, Xiao J, Gu W, Chen H - J Cancer (2016)

Bottom Line: Our data suggest that there is a significant difference in the expression levels in the liver between female and male mice.Several important genes in the gene network of Egfr are differentially expressed between female and male mice.The regulatory elements for the expression levels of Egfr between female and male mice are also different.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Orthopedic Surgery and BME-Campbell Clinic, University of Tennessee Health Science Center, Memphis, Tennessee, 38163, USA.; 2. Department of Basic Medical Research, Inner Mongolia Medical University, Inner Mongolia, 010110, China.

ABSTRACT
Epidermal growth factor receptor (EGFR) has been used as the target in drug design for cancer treatment including the liver cancer. Men and women have different levels of EGFR expression during the life. The whole genome expression profiles of livers of recombinant inbred (RI) strains derived from C57BL/6J (B6) X DBA/2J (D2) were used to compare three major molecular aspects of Egfr gene: the relative expression levels, gene network and eQTLs that regulate the expression of Egfr between female and male mice. Our data suggest that there is a significant difference in the expression levels in the liver between female and male mice. Several important genes in the gene network of Egfr are differentially expressed between female and male mice. The regulatory elements for the expression levels of Egfr between female and male mice are also different. In summary, our data reveals an important sex difference in the Egfr pathways in the liver of the mice. These data may have substantial impact on drug development and dosage determinant for women and men in the clinical trials.

No MeSH data available.


Related in: MedlinePlus

Expression levels of Egfr in the liver between the female and male mice in the BXD strains. Numbers on Y-axis are for the relative levels of the expression of Egfr. The information of strains and names of standard inbred strains are listed under the X-axis. A. The expression levels of Egfr in the liver of the female mice. B. The expression levels of Egfr in the liver of the male mice.
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Figure 2: Expression levels of Egfr in the liver between the female and male mice in the BXD strains. Numbers on Y-axis are for the relative levels of the expression of Egfr. The information of strains and names of standard inbred strains are listed under the X-axis. A. The expression levels of Egfr in the liver of the female mice. B. The expression levels of Egfr in the liver of the male mice.

Mentions: We conducted statistical analysis using data of gene expression of livers from a total of 39 strains, which have both the data from Actin β (beta) and Egfr in both sexes. There is one probe for Actin β on the Agilent G4121A chip. Figure 1A and 1B showed the expression levels of Actin β in female and male mice of different strains. For Actin B, the P value for T test between female and male is 0.88. The R value from the correlation analysis is 0.60. These data indicated that there is a high correlation in the expression levels of Actin β between male and female mice. Similarly, we obtained one probe for Egfr on the UNC Agilent G4121A chip. Figure 2A and 2B showed the expression levels of Egfr in the female and male mice of different strains. We also conducted the same statistical analyses on the levels of Egfr expression between the male and female mice. For Egfr, the P value for T test between the female and male is 1.07488E-17. The R value from the correlation analysis is 0.31. Therefore, there seems a difference in the expression levels between the male and female mice. Thus, there is no difference in the expression level of Actin β between the female and male populations while there is a significant sex difference in the Egfr expression levels. Therefore, further analyses were conducted as shown in the next several sections.


Sex Difference of Egfr Expression and Molecular Pathway in the Liver: Impact on Drug Design and Cancer Treatments?

Wang L, Xiao J, Gu W, Chen H - J Cancer (2016)

Expression levels of Egfr in the liver between the female and male mice in the BXD strains. Numbers on Y-axis are for the relative levels of the expression of Egfr. The information of strains and names of standard inbred strains are listed under the X-axis. A. The expression levels of Egfr in the liver of the female mice. B. The expression levels of Egfr in the liver of the male mice.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4829553&req=5

Figure 2: Expression levels of Egfr in the liver between the female and male mice in the BXD strains. Numbers on Y-axis are for the relative levels of the expression of Egfr. The information of strains and names of standard inbred strains are listed under the X-axis. A. The expression levels of Egfr in the liver of the female mice. B. The expression levels of Egfr in the liver of the male mice.
Mentions: We conducted statistical analysis using data of gene expression of livers from a total of 39 strains, which have both the data from Actin β (beta) and Egfr in both sexes. There is one probe for Actin β on the Agilent G4121A chip. Figure 1A and 1B showed the expression levels of Actin β in female and male mice of different strains. For Actin B, the P value for T test between female and male is 0.88. The R value from the correlation analysis is 0.60. These data indicated that there is a high correlation in the expression levels of Actin β between male and female mice. Similarly, we obtained one probe for Egfr on the UNC Agilent G4121A chip. Figure 2A and 2B showed the expression levels of Egfr in the female and male mice of different strains. We also conducted the same statistical analyses on the levels of Egfr expression between the male and female mice. For Egfr, the P value for T test between the female and male is 1.07488E-17. The R value from the correlation analysis is 0.31. Therefore, there seems a difference in the expression levels between the male and female mice. Thus, there is no difference in the expression level of Actin β between the female and male populations while there is a significant sex difference in the Egfr expression levels. Therefore, further analyses were conducted as shown in the next several sections.

Bottom Line: Our data suggest that there is a significant difference in the expression levels in the liver between female and male mice.Several important genes in the gene network of Egfr are differentially expressed between female and male mice.The regulatory elements for the expression levels of Egfr between female and male mice are also different.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Orthopedic Surgery and BME-Campbell Clinic, University of Tennessee Health Science Center, Memphis, Tennessee, 38163, USA.; 2. Department of Basic Medical Research, Inner Mongolia Medical University, Inner Mongolia, 010110, China.

ABSTRACT
Epidermal growth factor receptor (EGFR) has been used as the target in drug design for cancer treatment including the liver cancer. Men and women have different levels of EGFR expression during the life. The whole genome expression profiles of livers of recombinant inbred (RI) strains derived from C57BL/6J (B6) X DBA/2J (D2) were used to compare three major molecular aspects of Egfr gene: the relative expression levels, gene network and eQTLs that regulate the expression of Egfr between female and male mice. Our data suggest that there is a significant difference in the expression levels in the liver between female and male mice. Several important genes in the gene network of Egfr are differentially expressed between female and male mice. The regulatory elements for the expression levels of Egfr between female and male mice are also different. In summary, our data reveals an important sex difference in the Egfr pathways in the liver of the mice. These data may have substantial impact on drug development and dosage determinant for women and men in the clinical trials.

No MeSH data available.


Related in: MedlinePlus