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Downregulated Expression of Long Non-Coding RNA LOC101926975 Impairs both Cell Proliferation and Cell Cycle and Its Clinical Implication in Hirschsprung Disease Patients.

Shen Z, Peng L, Zhu Z, Xie H, Zang R, Du C, Chen G, Li H, Xia Y, Tang W - Int J Med Sci (2016)

Bottom Line: LOC101926975 was significantly downregulated in HSCR tissues with excellent correlation with FGF1.Meanwhile, the AUC of LOC101926975 was 0.900 which presented great diagnostic value.Our study firstly investigates the potential function of LOC101926975 in HSCR and infers that LOC101926975 can distinguish HSCR from the normal ones.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated Nanjing Medical University, Nanjing 210008; 2. State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166, China.

ABSTRACT

Background: Long non-coding RNAs (lncRNAs) have been reported to participate in various diseases. Hirschsprung disease (HSCR) is a common digestive disease in the new born. However, the relationship between lncRNAs and HSCR remains unclarified.

Methods: We used qRT-PCR to detect the relative expression of LOC101926975 in 80 pairs of HSCR bowel tissues and matched normal bowel tissues. CCK-8 assay, transwell assay and flow cytometry were then used to evaluate the function in vitro by knocking down the LOC101926975 in SK-N-BE(2) cells. Receiver operating characteristic (ROC) curve was used to evaluate the potential diagnostic value of LOC101926975.

Results: LOC101926975 was significantly downregulated in HSCR tissues with excellent correlation with FGF1. Dysregulation of LOC101926975 suppressed cell proliferation and induced G0/G1 arrest without impact on cell apoptosis or migration. Meanwhile, the AUC of LOC101926975 was 0.900 which presented great diagnostic value.

Conclusions: Our study firstly investigates the potential function of LOC101926975 in HSCR and infers that LOC101926975 can distinguish HSCR from the normal ones.

No MeSH data available.


Related in: MedlinePlus

Relationship between FGF1 and LOC101926975. The expression of FGF1 was lower in HSCR tissues (A) and was correlated with the expression of LOC101926975 in control samples (B), HSCR tissues (C) and cells (D). * indicates significant difference (p<0.05)
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Figure 3: Relationship between FGF1 and LOC101926975. The expression of FGF1 was lower in HSCR tissues (A) and was correlated with the expression of LOC101926975 in control samples (B), HSCR tissues (C) and cells (D). * indicates significant difference (p<0.05)

Mentions: To explore the potential mechanism of LOC101926975 regulating biological process, we focused on FGF1 due to its near location on chromosome. FGF1 is a member of the fibroblast growth factor family, which plays key roles in cell proliferation and embryonic development 9. We found that the expression of FGF1 was also low in HSCR cases (Fig 3A). The correlation analysis showed that the association between FGF1 and LOC101926975 was evident in both controls and cases with the r value of 0.9844 and 0.9804 respectively and p value <0.0001 (Fig 3B, C). And the expression of FGF1 in LOC101926975 knockdown cells was lower than the control according to the results of qRT-PCR (Fig 3D). All above, hinted that LOC101926975 might regulate the expression of FGF1 and thus participated in HSCR.


Downregulated Expression of Long Non-Coding RNA LOC101926975 Impairs both Cell Proliferation and Cell Cycle and Its Clinical Implication in Hirschsprung Disease Patients.

Shen Z, Peng L, Zhu Z, Xie H, Zang R, Du C, Chen G, Li H, Xia Y, Tang W - Int J Med Sci (2016)

Relationship between FGF1 and LOC101926975. The expression of FGF1 was lower in HSCR tissues (A) and was correlated with the expression of LOC101926975 in control samples (B), HSCR tissues (C) and cells (D). * indicates significant difference (p<0.05)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4829542&req=5

Figure 3: Relationship between FGF1 and LOC101926975. The expression of FGF1 was lower in HSCR tissues (A) and was correlated with the expression of LOC101926975 in control samples (B), HSCR tissues (C) and cells (D). * indicates significant difference (p<0.05)
Mentions: To explore the potential mechanism of LOC101926975 regulating biological process, we focused on FGF1 due to its near location on chromosome. FGF1 is a member of the fibroblast growth factor family, which plays key roles in cell proliferation and embryonic development 9. We found that the expression of FGF1 was also low in HSCR cases (Fig 3A). The correlation analysis showed that the association between FGF1 and LOC101926975 was evident in both controls and cases with the r value of 0.9844 and 0.9804 respectively and p value <0.0001 (Fig 3B, C). And the expression of FGF1 in LOC101926975 knockdown cells was lower than the control according to the results of qRT-PCR (Fig 3D). All above, hinted that LOC101926975 might regulate the expression of FGF1 and thus participated in HSCR.

Bottom Line: LOC101926975 was significantly downregulated in HSCR tissues with excellent correlation with FGF1.Meanwhile, the AUC of LOC101926975 was 0.900 which presented great diagnostic value.Our study firstly investigates the potential function of LOC101926975 in HSCR and infers that LOC101926975 can distinguish HSCR from the normal ones.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated Nanjing Medical University, Nanjing 210008; 2. State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166, China.

ABSTRACT

Background: Long non-coding RNAs (lncRNAs) have been reported to participate in various diseases. Hirschsprung disease (HSCR) is a common digestive disease in the new born. However, the relationship between lncRNAs and HSCR remains unclarified.

Methods: We used qRT-PCR to detect the relative expression of LOC101926975 in 80 pairs of HSCR bowel tissues and matched normal bowel tissues. CCK-8 assay, transwell assay and flow cytometry were then used to evaluate the function in vitro by knocking down the LOC101926975 in SK-N-BE(2) cells. Receiver operating characteristic (ROC) curve was used to evaluate the potential diagnostic value of LOC101926975.

Results: LOC101926975 was significantly downregulated in HSCR tissues with excellent correlation with FGF1. Dysregulation of LOC101926975 suppressed cell proliferation and induced G0/G1 arrest without impact on cell apoptosis or migration. Meanwhile, the AUC of LOC101926975 was 0.900 which presented great diagnostic value.

Conclusions: Our study firstly investigates the potential function of LOC101926975 in HSCR and infers that LOC101926975 can distinguish HSCR from the normal ones.

No MeSH data available.


Related in: MedlinePlus