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Aberrant gene expression of heparanase in ventricular hypertrophy induced by monocrotaline in rats.

Ishiguro-Oonuma T, Suemoto M, Okada M, Yoshioka K, Hara Y, Hashizume K, Kizaki K - J. Vet. Med. Sci. (2015)

Bottom Line: Twenty-five days after the injection, the right ventricle and lung wet weights were increased in MCT-treated rats compared with the control.Northern blot hybridization showed that heparanase and MMP2 expression increased significantly in the right and left ventricles of MCT-treated rats, whereas MMP9 was not induced.These findings indicate that heparanase and MMP2 might play an important role in the development of MCT-induced cardiac hypertrophy.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Resources, Integrated Center for Sciences, Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan.

ABSTRACT
The gene expression levels of heparanase, matrix metalloproteinase 2 (MMP2) and MMP9 were examined in ventricles after treatment with monocrotaline (MCT) to induce cardiac hypertrophy in rats. Rats received a single intraperitoneal injection of MCT (60 mg/kg) or saline. Twenty-five days after the injection, the right ventricle and lung wet weights were increased in MCT-treated rats compared with the control. Histological analysis revealed cardiomyocyte hypertrophy in the right ventricle of MCT-treated rats. Northern blot hybridization showed that heparanase and MMP2 expression increased significantly in the right and left ventricles of MCT-treated rats, whereas MMP9 was not induced. These findings indicate that heparanase and MMP2 might play an important role in the development of MCT-induced cardiac hypertrophy.

No MeSH data available.


Related in: MedlinePlus

Expression of heparanase, MMP2 and MMP9 mRNA in right and left ventricles ofMCT-treated rats. Representative Northern blot image of heparanase, MMP2, MMP9 and GAPDHin the right (RV) and left (LV) ventricles of control and MCT-treated rats (A).Densitometric analyses of heparanase (B) and MMP2 (C) mRNA levels. Levels werenormalized to GAPDH. Data are given as the mean ± SEM (n=4–5). Data labeled withdifferent letters are significantly different from each other(P<0.05).
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fig_002: Expression of heparanase, MMP2 and MMP9 mRNA in right and left ventricles ofMCT-treated rats. Representative Northern blot image of heparanase, MMP2, MMP9 and GAPDHin the right (RV) and left (LV) ventricles of control and MCT-treated rats (A).Densitometric analyses of heparanase (B) and MMP2 (C) mRNA levels. Levels werenormalized to GAPDH. Data are given as the mean ± SEM (n=4–5). Data labeled withdifferent letters are significantly different from each other(P<0.05).

Mentions: Northern blot hybridization was used to investigate heparanase, MMP2 and MMP9 expression inthe right and left ventricles. Heparanase mRNA was slightly expressed in the right and leftventricles in control rats (Fig. 2A and 2BFig. 2.


Aberrant gene expression of heparanase in ventricular hypertrophy induced by monocrotaline in rats.

Ishiguro-Oonuma T, Suemoto M, Okada M, Yoshioka K, Hara Y, Hashizume K, Kizaki K - J. Vet. Med. Sci. (2015)

Expression of heparanase, MMP2 and MMP9 mRNA in right and left ventricles ofMCT-treated rats. Representative Northern blot image of heparanase, MMP2, MMP9 and GAPDHin the right (RV) and left (LV) ventricles of control and MCT-treated rats (A).Densitometric analyses of heparanase (B) and MMP2 (C) mRNA levels. Levels werenormalized to GAPDH. Data are given as the mean ± SEM (n=4–5). Data labeled withdifferent letters are significantly different from each other(P<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829525&req=5

fig_002: Expression of heparanase, MMP2 and MMP9 mRNA in right and left ventricles ofMCT-treated rats. Representative Northern blot image of heparanase, MMP2, MMP9 and GAPDHin the right (RV) and left (LV) ventricles of control and MCT-treated rats (A).Densitometric analyses of heparanase (B) and MMP2 (C) mRNA levels. Levels werenormalized to GAPDH. Data are given as the mean ± SEM (n=4–5). Data labeled withdifferent letters are significantly different from each other(P<0.05).
Mentions: Northern blot hybridization was used to investigate heparanase, MMP2 and MMP9 expression inthe right and left ventricles. Heparanase mRNA was slightly expressed in the right and leftventricles in control rats (Fig. 2A and 2BFig. 2.

Bottom Line: Twenty-five days after the injection, the right ventricle and lung wet weights were increased in MCT-treated rats compared with the control.Northern blot hybridization showed that heparanase and MMP2 expression increased significantly in the right and left ventricles of MCT-treated rats, whereas MMP9 was not induced.These findings indicate that heparanase and MMP2 might play an important role in the development of MCT-induced cardiac hypertrophy.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Resources, Integrated Center for Sciences, Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan.

ABSTRACT
The gene expression levels of heparanase, matrix metalloproteinase 2 (MMP2) and MMP9 were examined in ventricles after treatment with monocrotaline (MCT) to induce cardiac hypertrophy in rats. Rats received a single intraperitoneal injection of MCT (60 mg/kg) or saline. Twenty-five days after the injection, the right ventricle and lung wet weights were increased in MCT-treated rats compared with the control. Histological analysis revealed cardiomyocyte hypertrophy in the right ventricle of MCT-treated rats. Northern blot hybridization showed that heparanase and MMP2 expression increased significantly in the right and left ventricles of MCT-treated rats, whereas MMP9 was not induced. These findings indicate that heparanase and MMP2 might play an important role in the development of MCT-induced cardiac hypertrophy.

No MeSH data available.


Related in: MedlinePlus