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Aberrant gene expression of heparanase in ventricular hypertrophy induced by monocrotaline in rats.

Ishiguro-Oonuma T, Suemoto M, Okada M, Yoshioka K, Hara Y, Hashizume K, Kizaki K - J. Vet. Med. Sci. (2015)

Bottom Line: Twenty-five days after the injection, the right ventricle and lung wet weights were increased in MCT-treated rats compared with the control.Northern blot hybridization showed that heparanase and MMP2 expression increased significantly in the right and left ventricles of MCT-treated rats, whereas MMP9 was not induced.These findings indicate that heparanase and MMP2 might play an important role in the development of MCT-induced cardiac hypertrophy.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Resources, Integrated Center for Sciences, Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan.

ABSTRACT
The gene expression levels of heparanase, matrix metalloproteinase 2 (MMP2) and MMP9 were examined in ventricles after treatment with monocrotaline (MCT) to induce cardiac hypertrophy in rats. Rats received a single intraperitoneal injection of MCT (60 mg/kg) or saline. Twenty-five days after the injection, the right ventricle and lung wet weights were increased in MCT-treated rats compared with the control. Histological analysis revealed cardiomyocyte hypertrophy in the right ventricle of MCT-treated rats. Northern blot hybridization showed that heparanase and MMP2 expression increased significantly in the right and left ventricles of MCT-treated rats, whereas MMP9 was not induced. These findings indicate that heparanase and MMP2 might play an important role in the development of MCT-induced cardiac hypertrophy.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of ventricular tissue sections obtained from control and MCT-treatedrats. Representative photomicrographs of HE-stained sections of right (A and C) and left(B and D) ventricles treated with (A and B) or without (C and D) MCT. Scale barsrepresent 100 µm.
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fig_001: Photomicrographs of ventricular tissue sections obtained from control and MCT-treatedrats. Representative photomicrographs of HE-stained sections of right (A and C) and left(B and D) ventricles treated with (A and B) or without (C and D) MCT. Scale barsrepresent 100 µm.

Mentions: Data are presented as the mean ± SEM. RV: Right ventricle. LV: Left ventricle includinginterventricular septum. *P<0.05: compared with control group.


Aberrant gene expression of heparanase in ventricular hypertrophy induced by monocrotaline in rats.

Ishiguro-Oonuma T, Suemoto M, Okada M, Yoshioka K, Hara Y, Hashizume K, Kizaki K - J. Vet. Med. Sci. (2015)

Photomicrographs of ventricular tissue sections obtained from control and MCT-treatedrats. Representative photomicrographs of HE-stained sections of right (A and C) and left(B and D) ventricles treated with (A and B) or without (C and D) MCT. Scale barsrepresent 100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829525&req=5

fig_001: Photomicrographs of ventricular tissue sections obtained from control and MCT-treatedrats. Representative photomicrographs of HE-stained sections of right (A and C) and left(B and D) ventricles treated with (A and B) or without (C and D) MCT. Scale barsrepresent 100 µm.
Mentions: Data are presented as the mean ± SEM. RV: Right ventricle. LV: Left ventricle includinginterventricular septum. *P<0.05: compared with control group.

Bottom Line: Twenty-five days after the injection, the right ventricle and lung wet weights were increased in MCT-treated rats compared with the control.Northern blot hybridization showed that heparanase and MMP2 expression increased significantly in the right and left ventricles of MCT-treated rats, whereas MMP9 was not induced.These findings indicate that heparanase and MMP2 might play an important role in the development of MCT-induced cardiac hypertrophy.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Resources, Integrated Center for Sciences, Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan.

ABSTRACT
The gene expression levels of heparanase, matrix metalloproteinase 2 (MMP2) and MMP9 were examined in ventricles after treatment with monocrotaline (MCT) to induce cardiac hypertrophy in rats. Rats received a single intraperitoneal injection of MCT (60 mg/kg) or saline. Twenty-five days after the injection, the right ventricle and lung wet weights were increased in MCT-treated rats compared with the control. Histological analysis revealed cardiomyocyte hypertrophy in the right ventricle of MCT-treated rats. Northern blot hybridization showed that heparanase and MMP2 expression increased significantly in the right and left ventricles of MCT-treated rats, whereas MMP9 was not induced. These findings indicate that heparanase and MMP2 might play an important role in the development of MCT-induced cardiac hypertrophy.

No MeSH data available.


Related in: MedlinePlus