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The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan.

Iguchi A, Soma T, Suzuki H, Xuan X - J. Vet. Med. Sci. (2015)

Bottom Line: In this study, 3/73 samples showed over 5% M121I variant population.Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites.It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.

View Article: PubMed Central - PubMed

Affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.

ABSTRACT
In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against B. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-resistant B. gibsoni had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.

No MeSH data available.


Samples were collected from gray area. The number on the map showed sample numbers.Each 1 sample showed over 1% M121I variant population in dark gray areas (Osaka,Hiroshima and Yamaguchi).
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fig_001: Samples were collected from gray area. The number on the map showed sample numbers.Each 1 sample showed over 1% M121I variant population in dark gray areas (Osaka,Hiroshima and Yamaguchi).

Mentions: Seventy-three genomic DNA (gDNA) samples were collected from dogs naturally infected withB. gibsoni during 2011–2014. All samples were provided by a commerciallaboratory (Marupi Lifetech Co., Ltd., Osaka, Japan). These samples were collected fromclinically suspicious cases and confirmed to be positive for B. gibsoniinfection by following B. gibsoni P18 gene PCR [3]. Table 1Table 1.The age, sex and breeds of dogs shows the age, sex and breeds of dogs in these samples. There were noinformation about the dogs’ histories and treatment including usage of ATV. They werecollected from dogs mainly in areas west of Japan (Fukuoka, Oita, Yamaguchi, Hiroshima,Okayama, Hyogo, Ehime, Kochi, Kagawa, Osaka, Kyoto, Shiga, Mie, Wakayama and Aichi; only twosamples were from the eastern part, Ibaraki and Hokkaido; Fig. 1Fig. 1.


The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan.

Iguchi A, Soma T, Suzuki H, Xuan X - J. Vet. Med. Sci. (2015)

Samples were collected from gray area. The number on the map showed sample numbers.Each 1 sample showed over 1% M121I variant population in dark gray areas (Osaka,Hiroshima and Yamaguchi).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829523&req=5

fig_001: Samples were collected from gray area. The number on the map showed sample numbers.Each 1 sample showed over 1% M121I variant population in dark gray areas (Osaka,Hiroshima and Yamaguchi).
Mentions: Seventy-three genomic DNA (gDNA) samples were collected from dogs naturally infected withB. gibsoni during 2011–2014. All samples were provided by a commerciallaboratory (Marupi Lifetech Co., Ltd., Osaka, Japan). These samples were collected fromclinically suspicious cases and confirmed to be positive for B. gibsoniinfection by following B. gibsoni P18 gene PCR [3]. Table 1Table 1.The age, sex and breeds of dogs shows the age, sex and breeds of dogs in these samples. There were noinformation about the dogs’ histories and treatment including usage of ATV. They werecollected from dogs mainly in areas west of Japan (Fukuoka, Oita, Yamaguchi, Hiroshima,Okayama, Hyogo, Ehime, Kochi, Kagawa, Osaka, Kyoto, Shiga, Mie, Wakayama and Aichi; only twosamples were from the eastern part, Ibaraki and Hokkaido; Fig. 1Fig. 1.

Bottom Line: In this study, 3/73 samples showed over 5% M121I variant population.Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites.It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.

View Article: PubMed Central - PubMed

Affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.

ABSTRACT
In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against B. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-resistant B. gibsoni had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.

No MeSH data available.