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Growth kinetics of Salmonella enterica in Hajna tetrathionate broth, Rappaport broth and modified semisolid Rappaport agar.

Fujihara M, Tabuchi H, Uegaki K - J. Vet. Med. Sci. (2015)

Bottom Line: S. enterica was more successfully isolated from artificially contaminated fecal samples after enrichment in Hajna tetrathionate broth or modified semisolid Rappaport agar than in Rappaport broth.Since most bacteria (other than motile S. enterica) do not migrate on modified semisolid Rappaport agar, the growth characteristics of S. enterica can be interpreted easily and quickly.Two S. enterica isolates did not migrate on modified semisolid Rappaport agar, but did grow in Hajna tetrathionate broth, which suggests that the combined use of these selective enrichment media is appropriate for isolating S. enterica.

View Article: PubMed Central - PubMed

Affiliation: Hokkaido Hiyama Livestock Hygiene Service Center, 281-1 Tazawamachi, Esashi, Hiyamagun, Hokkaido 043-0023, Japan.

ABSTRACT
To determine the appropriate method for isolating Salmonella enterica, we compared the growth of S. enterica serovars using three selective enrichment media. S. enterica was more successfully isolated from artificially contaminated fecal samples after enrichment in Hajna tetrathionate broth or modified semisolid Rappaport agar than in Rappaport broth. Since most bacteria (other than motile S. enterica) do not migrate on modified semisolid Rappaport agar, the growth characteristics of S. enterica can be interpreted easily and quickly. Two S. enterica isolates did not migrate on modified semisolid Rappaport agar, but did grow in Hajna tetrathionate broth, which suggests that the combined use of these selective enrichment media is appropriate for isolating S. enterica.

No MeSH data available.


Migration of S. enterica from artificially contaminated bovine feceson MSRA after 17 hr incubation at 40°C. Values represent the additive CFU ofS. Livingstone.
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fig_001: Migration of S. enterica from artificially contaminated bovine feceson MSRA after 17 hr incubation at 40°C. Values represent the additive CFU ofS. Livingstone.

Mentions: *The numbers in parentheses indicate the S. enterica dominant (thehighest S. enterica colony count on subcultured agar plates).


Growth kinetics of Salmonella enterica in Hajna tetrathionate broth, Rappaport broth and modified semisolid Rappaport agar.

Fujihara M, Tabuchi H, Uegaki K - J. Vet. Med. Sci. (2015)

Migration of S. enterica from artificially contaminated bovine feceson MSRA after 17 hr incubation at 40°C. Values represent the additive CFU ofS. Livingstone.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829512&req=5

fig_001: Migration of S. enterica from artificially contaminated bovine feceson MSRA after 17 hr incubation at 40°C. Values represent the additive CFU ofS. Livingstone.
Mentions: *The numbers in parentheses indicate the S. enterica dominant (thehighest S. enterica colony count on subcultured agar plates).

Bottom Line: S. enterica was more successfully isolated from artificially contaminated fecal samples after enrichment in Hajna tetrathionate broth or modified semisolid Rappaport agar than in Rappaport broth.Since most bacteria (other than motile S. enterica) do not migrate on modified semisolid Rappaport agar, the growth characteristics of S. enterica can be interpreted easily and quickly.Two S. enterica isolates did not migrate on modified semisolid Rappaport agar, but did grow in Hajna tetrathionate broth, which suggests that the combined use of these selective enrichment media is appropriate for isolating S. enterica.

View Article: PubMed Central - PubMed

Affiliation: Hokkaido Hiyama Livestock Hygiene Service Center, 281-1 Tazawamachi, Esashi, Hiyamagun, Hokkaido 043-0023, Japan.

ABSTRACT
To determine the appropriate method for isolating Salmonella enterica, we compared the growth of S. enterica serovars using three selective enrichment media. S. enterica was more successfully isolated from artificially contaminated fecal samples after enrichment in Hajna tetrathionate broth or modified semisolid Rappaport agar than in Rappaport broth. Since most bacteria (other than motile S. enterica) do not migrate on modified semisolid Rappaport agar, the growth characteristics of S. enterica can be interpreted easily and quickly. Two S. enterica isolates did not migrate on modified semisolid Rappaport agar, but did grow in Hajna tetrathionate broth, which suggests that the combined use of these selective enrichment media is appropriate for isolating S. enterica.

No MeSH data available.