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A study of the osmotic characteristics, water permeability, and cryoprotectant permeability of human vaginal immune cells.

Shu Z, Hughes SM, Fang C, Huang J, Fu B, Zhao G, Fialkow M, Lentz G, Hladik F, Gao D - Cryobiology (2016)

Bottom Line: Results indicated Vb values of 0.516 V0 and 0.457 V0 for mucosal T cells and macrophages, respectively.Lp values at room temperature were 0.196 and 0.295 μm/min/atm for T cells and macrophages, respectively.Both cell types had high Ps values for the three CPAs, dimethyl sulfoxide (DMSO), propylene glycol (PG) and ethylene glycol (EG) (minimum of 0.418 × 10(-3) cm/min), but transport of the fourth CPA, glycerol, occurred 50-150 times more slowly.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA; School of Mechanical and Materials Engineering, Washington State University, Everett, WA 98201, USA.

No MeSH data available.


Related in: MedlinePlus

CPA exposure tolerance tests for human vaginal mucosal T cells and macrophages. The x-axis shows the final CPA concentration (v/v, %) after addition into the cell suspensions. The cell viabilities were tested with flow cytometry after CPA addition and removal, and the results were normalized to the viability of fresh cells (mean ± STD, n = 4–5).
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fig4: CPA exposure tolerance tests for human vaginal mucosal T cells and macrophages. The x-axis shows the final CPA concentration (v/v, %) after addition into the cell suspensions. The cell viabilities were tested with flow cytometry after CPA addition and removal, and the results were normalized to the viability of fresh cells (mean ± STD, n = 4–5).

Mentions: To determine the CPA exposure tolerance of mucosal cells, we added DMSO, EG, or PG at various concentrations dropwise to cell suspensions, incubated for 10 min on ice, and then dropwise diluted them out. We measured viability by cell type with flow cytometry, normalizing to the viability of untreated cells to have a consistent measure across samples. Fig. 4 shows the CPA exposure tolerance for human vaginal mucosal T cells and macrophages. The cell viability declined in a linear fashion as CPA concentrations increased. The relative viabilities remained above 90% up to concentrations of about 10% (v/v). DMSO had a more negative effect on T cell viability than the other two CPAs, while EG had a more negative effect on macrophage viability than the other two.


A study of the osmotic characteristics, water permeability, and cryoprotectant permeability of human vaginal immune cells.

Shu Z, Hughes SM, Fang C, Huang J, Fu B, Zhao G, Fialkow M, Lentz G, Hladik F, Gao D - Cryobiology (2016)

CPA exposure tolerance tests for human vaginal mucosal T cells and macrophages. The x-axis shows the final CPA concentration (v/v, %) after addition into the cell suspensions. The cell viabilities were tested with flow cytometry after CPA addition and removal, and the results were normalized to the viability of fresh cells (mean ± STD, n = 4–5).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829375&req=5

fig4: CPA exposure tolerance tests for human vaginal mucosal T cells and macrophages. The x-axis shows the final CPA concentration (v/v, %) after addition into the cell suspensions. The cell viabilities were tested with flow cytometry after CPA addition and removal, and the results were normalized to the viability of fresh cells (mean ± STD, n = 4–5).
Mentions: To determine the CPA exposure tolerance of mucosal cells, we added DMSO, EG, or PG at various concentrations dropwise to cell suspensions, incubated for 10 min on ice, and then dropwise diluted them out. We measured viability by cell type with flow cytometry, normalizing to the viability of untreated cells to have a consistent measure across samples. Fig. 4 shows the CPA exposure tolerance for human vaginal mucosal T cells and macrophages. The cell viability declined in a linear fashion as CPA concentrations increased. The relative viabilities remained above 90% up to concentrations of about 10% (v/v). DMSO had a more negative effect on T cell viability than the other two CPAs, while EG had a more negative effect on macrophage viability than the other two.

Bottom Line: Results indicated Vb values of 0.516 V0 and 0.457 V0 for mucosal T cells and macrophages, respectively.Lp values at room temperature were 0.196 and 0.295 μm/min/atm for T cells and macrophages, respectively.Both cell types had high Ps values for the three CPAs, dimethyl sulfoxide (DMSO), propylene glycol (PG) and ethylene glycol (EG) (minimum of 0.418 × 10(-3) cm/min), but transport of the fourth CPA, glycerol, occurred 50-150 times more slowly.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA; School of Mechanical and Materials Engineering, Washington State University, Everett, WA 98201, USA.

No MeSH data available.


Related in: MedlinePlus