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Comparison of Neurological Function in Males and Females from Two Substrains of C57BL/6 Mice.

Ashworth A, Bardgett ME, Fowler J, Garber H, Griffith M, Curran CP - Toxics (2014)

Bottom Line: However, B6N females had significantly shorter path lengths in the reversal and shifted-reduced phases.In the fear conditioning test, B6N males had a significantly longer time freezing in the new context compared with B6J males, but no significant differences were found in contextual or cued tests.Both factors (sex and substrain) must be taken into account when designing developmental neurotoxicology studies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biological Sciences, Northern Kentucky University, SC344 Nunn Dr, Highland Heights, KY 41099, USA.

ABSTRACT

The C57BL/6 (B6) mouse is the background strain most frequently used for genetically-modified mice. Previous studies have found significant behavioral and genetic differences between the B6J (The Jackson Laboratory) and B6N substrains (National Institutes of Health); however, most studies employed only male mice. We performed a comprehensive battery of motor function and learning and memory tests on male and female mice from both substrains. The B6N male mice had greater improvement in the rotarod test. In contrast, B6J female mice had longer latencies to falling from the rotarod. In the Morris water maze (MWM), B6J males had significantly shorter latencies to finding the hidden platform. However, B6N females had significantly shorter path lengths in the reversal and shifted-reduced phases. In open field locomotor activity, B6J males had higher activity levels, whereas B6N females took longer to habituate. In the fear conditioning test, B6N males had a significantly longer time freezing in the new context compared with B6J males, but no significant differences were found in contextual or cued tests. In summary, our findings demonstrate the importance of testing both males and females in neurobehavioral studies. Both factors (sex and substrain) must be taken into account when designing developmental neurotoxicology studies.

No MeSH data available.


Related in: MedlinePlus

(A,B) Rotarod. B6N males showed a greater percent increase in the latency to falling from Day 1 to Day 5 of testing compared with B6J males, and there was a significant effect of day (F4,144 = 12.75; p < 0.0001). B6N female mice also showed a greater percent improvement in the latency to falling over the five days of testing and significantly longer latencies to falling on Days 4 and 5. * p < 0.05, ** p < 0.01. Data are the least square means ± S.E.M.
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Figure 2: (A,B) Rotarod. B6N males showed a greater percent increase in the latency to falling from Day 1 to Day 5 of testing compared with B6J males, and there was a significant effect of day (F4,144 = 12.75; p < 0.0001). B6N female mice also showed a greater percent improvement in the latency to falling over the five days of testing and significantly longer latencies to falling on Days 4 and 5. * p < 0.05, ** p < 0.01. Data are the least square means ± S.E.M.

Mentions: There were no significant differences on any of the five days of testing when B6J and B6N males were tested; however, B6N males had a greater percent increase in the latency to falling over the five-day test compared with B6J males (Figure 2A). B6N males improved 53% by Day 5, whereas B6J males only improved 24%. For female mice, there was a significant gene × day interaction (F4,122 = 4.97; p = 0.001) (Figure 2B). B6N females had a 76% increase in the latency to falling across the five days of testing compared with a 16% increase for B6J females.


Comparison of Neurological Function in Males and Females from Two Substrains of C57BL/6 Mice.

Ashworth A, Bardgett ME, Fowler J, Garber H, Griffith M, Curran CP - Toxics (2014)

(A,B) Rotarod. B6N males showed a greater percent increase in the latency to falling from Day 1 to Day 5 of testing compared with B6J males, and there was a significant effect of day (F4,144 = 12.75; p < 0.0001). B6N female mice also showed a greater percent improvement in the latency to falling over the five days of testing and significantly longer latencies to falling on Days 4 and 5. * p < 0.05, ** p < 0.01. Data are the least square means ± S.E.M.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829364&req=5

Figure 2: (A,B) Rotarod. B6N males showed a greater percent increase in the latency to falling from Day 1 to Day 5 of testing compared with B6J males, and there was a significant effect of day (F4,144 = 12.75; p < 0.0001). B6N female mice also showed a greater percent improvement in the latency to falling over the five days of testing and significantly longer latencies to falling on Days 4 and 5. * p < 0.05, ** p < 0.01. Data are the least square means ± S.E.M.
Mentions: There were no significant differences on any of the five days of testing when B6J and B6N males were tested; however, B6N males had a greater percent increase in the latency to falling over the five-day test compared with B6J males (Figure 2A). B6N males improved 53% by Day 5, whereas B6J males only improved 24%. For female mice, there was a significant gene × day interaction (F4,122 = 4.97; p = 0.001) (Figure 2B). B6N females had a 76% increase in the latency to falling across the five days of testing compared with a 16% increase for B6J females.

Bottom Line: However, B6N females had significantly shorter path lengths in the reversal and shifted-reduced phases.In the fear conditioning test, B6N males had a significantly longer time freezing in the new context compared with B6J males, but no significant differences were found in contextual or cued tests.Both factors (sex and substrain) must be taken into account when designing developmental neurotoxicology studies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biological Sciences, Northern Kentucky University, SC344 Nunn Dr, Highland Heights, KY 41099, USA.

ABSTRACT

The C57BL/6 (B6) mouse is the background strain most frequently used for genetically-modified mice. Previous studies have found significant behavioral and genetic differences between the B6J (The Jackson Laboratory) and B6N substrains (National Institutes of Health); however, most studies employed only male mice. We performed a comprehensive battery of motor function and learning and memory tests on male and female mice from both substrains. The B6N male mice had greater improvement in the rotarod test. In contrast, B6J female mice had longer latencies to falling from the rotarod. In the Morris water maze (MWM), B6J males had significantly shorter latencies to finding the hidden platform. However, B6N females had significantly shorter path lengths in the reversal and shifted-reduced phases. In open field locomotor activity, B6J males had higher activity levels, whereas B6N females took longer to habituate. In the fear conditioning test, B6N males had a significantly longer time freezing in the new context compared with B6J males, but no significant differences were found in contextual or cued tests. In summary, our findings demonstrate the importance of testing both males and females in neurobehavioral studies. Both factors (sex and substrain) must be taken into account when designing developmental neurotoxicology studies.

No MeSH data available.


Related in: MedlinePlus