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Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide.

Wu T, Wang C, Ding L, Shen Y, Cui H, Wang M, Wang H - Mediators Inflamm. (2016)

Bottom Line: The results showed that arginine reduced the LPS-induced production like IL-1β, IL-6, TNF-α, and iNOS.Though the expression of NF-κB was attenuated and the mTOR signaling pathway was upregulated, arginine had no effect on TLR4 expression.In addition, our results show that the content of β-casein and the total casein were enhanced after arginine was supplemented in LPS-induced BMECs.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

ABSTRACT
As one of functional active amino acids, L-arginine holds a key position in immunity. However, the mechanism that arginine modulates cow mammary inflammatory response in ruminant is unclear. Therefore, this study was conducted to investigate the effects of L-arginine on inflammatory response and casein expression after challenging the bovine mammary epithelial cells (BMECs) with lipopolysaccharide (LPS). The cells were divided into four groups, stimulated with or without LPS (10 μg/mL) and treated with or without arginine (100 μg/mL) for 12 h. The concentration of proinflammatory cytokines, inducible nitric oxide synthase (iNOS), mammalian target of rapamycin (mTOR), and Toll-like receptor 4 (TLR4) signaling pathways as well as the casein was determined. The results showed that arginine reduced the LPS-induced production like IL-1β, IL-6, TNF-α, and iNOS. Though the expression of NF-κB was attenuated and the mTOR signaling pathway was upregulated, arginine had no effect on TLR4 expression. In addition, our results show that the content of β-casein and the total casein were enhanced after arginine was supplemented in LPS-induced BMECs. In conclusion, arginine could relieve the inflammatory reaction induced by LPS and enhance the concentration of β-casein and the total casein in bovine mammary epithelial cells.

No MeSH data available.


Related in: MedlinePlus

The potential mechanism that arginine relieves the inflammatory response and enhances the casein expression in bovine mammary epithelial cells induced by lipopolysaccharide.
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Related In: Results  -  Collection


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fig5: The potential mechanism that arginine relieves the inflammatory response and enhances the casein expression in bovine mammary epithelial cells induced by lipopolysaccharide.

Mentions: In summary, the results from the present experiment implied that arginine effectively attenuated LPS-induced bovine mammary epithelial cells inflammatory response by inhibiting NF-κB signaling pathways. Additionally, arginine may be involved in Arg/NO and PI3K/AKT/mTOR pathway. Further, arginine was also able to enhance the β-casein and the total casein expression in LPS-induced bovine mammary epithelial cells (Figure 5).


Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide.

Wu T, Wang C, Ding L, Shen Y, Cui H, Wang M, Wang H - Mediators Inflamm. (2016)

The potential mechanism that arginine relieves the inflammatory response and enhances the casein expression in bovine mammary epithelial cells induced by lipopolysaccharide.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4821974&req=5

fig5: The potential mechanism that arginine relieves the inflammatory response and enhances the casein expression in bovine mammary epithelial cells induced by lipopolysaccharide.
Mentions: In summary, the results from the present experiment implied that arginine effectively attenuated LPS-induced bovine mammary epithelial cells inflammatory response by inhibiting NF-κB signaling pathways. Additionally, arginine may be involved in Arg/NO and PI3K/AKT/mTOR pathway. Further, arginine was also able to enhance the β-casein and the total casein expression in LPS-induced bovine mammary epithelial cells (Figure 5).

Bottom Line: The results showed that arginine reduced the LPS-induced production like IL-1β, IL-6, TNF-α, and iNOS.Though the expression of NF-κB was attenuated and the mTOR signaling pathway was upregulated, arginine had no effect on TLR4 expression.In addition, our results show that the content of β-casein and the total casein were enhanced after arginine was supplemented in LPS-induced BMECs.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

ABSTRACT
As one of functional active amino acids, L-arginine holds a key position in immunity. However, the mechanism that arginine modulates cow mammary inflammatory response in ruminant is unclear. Therefore, this study was conducted to investigate the effects of L-arginine on inflammatory response and casein expression after challenging the bovine mammary epithelial cells (BMECs) with lipopolysaccharide (LPS). The cells were divided into four groups, stimulated with or without LPS (10 μg/mL) and treated with or without arginine (100 μg/mL) for 12 h. The concentration of proinflammatory cytokines, inducible nitric oxide synthase (iNOS), mammalian target of rapamycin (mTOR), and Toll-like receptor 4 (TLR4) signaling pathways as well as the casein was determined. The results showed that arginine reduced the LPS-induced production like IL-1β, IL-6, TNF-α, and iNOS. Though the expression of NF-κB was attenuated and the mTOR signaling pathway was upregulated, arginine had no effect on TLR4 expression. In addition, our results show that the content of β-casein and the total casein were enhanced after arginine was supplemented in LPS-induced BMECs. In conclusion, arginine could relieve the inflammatory reaction induced by LPS and enhance the concentration of β-casein and the total casein in bovine mammary epithelial cells.

No MeSH data available.


Related in: MedlinePlus