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HSP27 Alleviates Cardiac Aging in Mice via a Mechanism Involving Antioxidation and Mitophagy Activation.

Lin S, Wang Y, Zhang X, Kong Q, Li C, Li Y, Ding Z, Liu L - Oxid Med Cell Longev (2016)

Bottom Line: Levels of Atg13, Vps34, and Rab7 were also higher in old Tg hearts than in old WT hearts.Additionally, old Tg hearts had higher levels of PINK1 and Parkin than old WT hearts, suggesting that mitophagy was activated in old Tg hearts.Taken together, HSP27 alleviated cardiac aging and this action involved antioxidation and mitophagy activation.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics, First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China.

ABSTRACT
Aging-induced cardiac dysfunction is a prominent feature of cardiac aging. Heat shock protein 27 (HSP27) protects cardiac function against ischemia or chemical challenge. We hypothesized that HSP27 attenuates cardiac aging. Transgenic (Tg) mice with cardiac-specific expression of the HSP27 gene and wild-type (WT) littermates were employed in the experiments. Echocardiography revealed a significant decline in the cardiac function of old WT mice compared with young WT mice. In striking contrast, the aging-induced impairment of cardiac function was attenuated in old Tg mice compared with old WT mice. Levels of cardiac aging markers were lower in old Tg mouse hearts than in old WT mouse hearts. Less interstitial fibrosis and lower contents of reactive oxygen species and ubiquitin-conjugated proteins were detected in old Tg hearts than in old WT hearts. Furthermore, old Tg hearts demonstrated lower accumulation of LC3-II and p62 than old WT hearts. Levels of Atg13, Vps34, and Rab7 were also higher in old Tg hearts than in old WT hearts. Additionally, old Tg hearts had higher levels of PINK1 and Parkin than old WT hearts, suggesting that mitophagy was activated in old Tg hearts. Taken together, HSP27 alleviated cardiac aging and this action involved antioxidation and mitophagy activation.

No MeSH data available.


Related in: MedlinePlus

HSP27 increases autophagic markers in the hearts of old mice. The hearts were collected from 24-month-old mice. Protein extracts were prepared for immunoblot analysis against LC3 and p62 (a) and against Atg 13, Vps34, and Rab7 (b). The blots against GAPDH served as loading controls. ∗∗P < 0.01 versus WT controls. n = 3 per group.
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fig5: HSP27 increases autophagic markers in the hearts of old mice. The hearts were collected from 24-month-old mice. Protein extracts were prepared for immunoblot analysis against LC3 and p62 (a) and against Atg 13, Vps34, and Rab7 (b). The blots against GAPDH served as loading controls. ∗∗P < 0.01 versus WT controls. n = 3 per group.

Mentions: Activation of mitochondrial autophagy (mitophagy) alleviates cardiac aging [24]. We first examined LC3 conversion and p62 protein degradation, two widely used markers of autophagic flux [25], in hearts by immunoblot analysis. The level of LC3-II was significantly decreased by 38.7% in old Tg mouse hearts compared with old WT mouse hearts (P < 0.01) (Figure 5(a)). Immunostaining of LC3-II confirmed the observation made in immunoblot analysis (Figure 5(b)). By contrast, the p62 protein level was reduced by 59.0% in old Tg mouse hearts compared with old WT mouse hearts (P < 0.01). These data suggest that autophagic flux was significantly higher in old Tg mouse hearts than in old WT mouse hearts.


HSP27 Alleviates Cardiac Aging in Mice via a Mechanism Involving Antioxidation and Mitophagy Activation.

Lin S, Wang Y, Zhang X, Kong Q, Li C, Li Y, Ding Z, Liu L - Oxid Med Cell Longev (2016)

HSP27 increases autophagic markers in the hearts of old mice. The hearts were collected from 24-month-old mice. Protein extracts were prepared for immunoblot analysis against LC3 and p62 (a) and against Atg 13, Vps34, and Rab7 (b). The blots against GAPDH served as loading controls. ∗∗P < 0.01 versus WT controls. n = 3 per group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4821973&req=5

fig5: HSP27 increases autophagic markers in the hearts of old mice. The hearts were collected from 24-month-old mice. Protein extracts were prepared for immunoblot analysis against LC3 and p62 (a) and against Atg 13, Vps34, and Rab7 (b). The blots against GAPDH served as loading controls. ∗∗P < 0.01 versus WT controls. n = 3 per group.
Mentions: Activation of mitochondrial autophagy (mitophagy) alleviates cardiac aging [24]. We first examined LC3 conversion and p62 protein degradation, two widely used markers of autophagic flux [25], in hearts by immunoblot analysis. The level of LC3-II was significantly decreased by 38.7% in old Tg mouse hearts compared with old WT mouse hearts (P < 0.01) (Figure 5(a)). Immunostaining of LC3-II confirmed the observation made in immunoblot analysis (Figure 5(b)). By contrast, the p62 protein level was reduced by 59.0% in old Tg mouse hearts compared with old WT mouse hearts (P < 0.01). These data suggest that autophagic flux was significantly higher in old Tg mouse hearts than in old WT mouse hearts.

Bottom Line: Levels of Atg13, Vps34, and Rab7 were also higher in old Tg hearts than in old WT hearts.Additionally, old Tg hearts had higher levels of PINK1 and Parkin than old WT hearts, suggesting that mitophagy was activated in old Tg hearts.Taken together, HSP27 alleviated cardiac aging and this action involved antioxidation and mitophagy activation.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics, First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China.

ABSTRACT
Aging-induced cardiac dysfunction is a prominent feature of cardiac aging. Heat shock protein 27 (HSP27) protects cardiac function against ischemia or chemical challenge. We hypothesized that HSP27 attenuates cardiac aging. Transgenic (Tg) mice with cardiac-specific expression of the HSP27 gene and wild-type (WT) littermates were employed in the experiments. Echocardiography revealed a significant decline in the cardiac function of old WT mice compared with young WT mice. In striking contrast, the aging-induced impairment of cardiac function was attenuated in old Tg mice compared with old WT mice. Levels of cardiac aging markers were lower in old Tg mouse hearts than in old WT mouse hearts. Less interstitial fibrosis and lower contents of reactive oxygen species and ubiquitin-conjugated proteins were detected in old Tg hearts than in old WT hearts. Furthermore, old Tg hearts demonstrated lower accumulation of LC3-II and p62 than old WT hearts. Levels of Atg13, Vps34, and Rab7 were also higher in old Tg hearts than in old WT hearts. Additionally, old Tg hearts had higher levels of PINK1 and Parkin than old WT hearts, suggesting that mitophagy was activated in old Tg hearts. Taken together, HSP27 alleviated cardiac aging and this action involved antioxidation and mitophagy activation.

No MeSH data available.


Related in: MedlinePlus