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Primary Multidrug Resistant Tuberculosis and Utility of Line Probe Assay for Its Detection in Smear-Positive Sputum Samples in a Tertiary Care Hospital in South India.

Yacoob FL, Philomina Jose B, Karunakaran Lelitha SD, Sreenivasan S - J Pathog (2016)

Bottom Line: All samples were inoculated onto solid media and 61 samples were inoculated in automated liquid culture also.Only 1% of the cases were primary multidrug resistant tuberculosis and 1.5% showed isoniazid monoresistance.S531L and C15T were the most common genetic mutations seen for rifampicin and isoniazid resistance, respectively. 40% had absent rpoB wild type 8 band indicating probable silent mutation after clinical correlation.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Government Medical College, Kozhikode, Kerala 673008, India.

ABSTRACT
In a high tuberculosis burdened country like India, rapid, cost-effective, and reliable diagnostic tools for tuberculosis are an urgent need of the hour to prevent inappropriate treatment strategies and further spread of resistance. This study aimed to estimate the proportion of new smear-positive tuberculosis cases with primary resistance to rifampicin and/or isoniazid as well as identify the common mutations associated with it. Sputum of 200 newly diagnosed smear-positive cases of 1+ score and above was directly subjected to Line Probe Assay using the GenoType MTBDRplus assay kit. All samples were inoculated onto solid media and 61 samples were inoculated in automated liquid culture also. The Line Probe Assay gave hundred percent interpretable results with 2.5% of the study population showing resistant pattern. Only 1% of the cases were primary multidrug resistant tuberculosis and 1.5% showed isoniazid monoresistance. S531L and C15T were the most common genetic mutations seen for rifampicin and isoniazid resistance, respectively. 40% had absent rpoB wild type 8 band indicating probable silent mutation after clinical correlation. The average turnaround time for Line Probe Assay was far less (3.8 days) as compared to solid and liquid cultures (35.6 days and 13.5 days, resp.).

No MeSH data available.


Related in: MedlinePlus

Age and gender distribution of the study population. The majority of the patients are of the age group between 41 and 50 with male preponderance.
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Related In: Results  -  Collection


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fig7: Age and gender distribution of the study population. The majority of the patients are of the age group between 41 and 50 with male preponderance.

Mentions: The age and sex distribution of the study population is depicted in Figure 7.


Primary Multidrug Resistant Tuberculosis and Utility of Line Probe Assay for Its Detection in Smear-Positive Sputum Samples in a Tertiary Care Hospital in South India.

Yacoob FL, Philomina Jose B, Karunakaran Lelitha SD, Sreenivasan S - J Pathog (2016)

Age and gender distribution of the study population. The majority of the patients are of the age group between 41 and 50 with male preponderance.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4821965&req=5

fig7: Age and gender distribution of the study population. The majority of the patients are of the age group between 41 and 50 with male preponderance.
Mentions: The age and sex distribution of the study population is depicted in Figure 7.

Bottom Line: All samples were inoculated onto solid media and 61 samples were inoculated in automated liquid culture also.Only 1% of the cases were primary multidrug resistant tuberculosis and 1.5% showed isoniazid monoresistance.S531L and C15T were the most common genetic mutations seen for rifampicin and isoniazid resistance, respectively. 40% had absent rpoB wild type 8 band indicating probable silent mutation after clinical correlation.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Government Medical College, Kozhikode, Kerala 673008, India.

ABSTRACT
In a high tuberculosis burdened country like India, rapid, cost-effective, and reliable diagnostic tools for tuberculosis are an urgent need of the hour to prevent inappropriate treatment strategies and further spread of resistance. This study aimed to estimate the proportion of new smear-positive tuberculosis cases with primary resistance to rifampicin and/or isoniazid as well as identify the common mutations associated with it. Sputum of 200 newly diagnosed smear-positive cases of 1+ score and above was directly subjected to Line Probe Assay using the GenoType MTBDRplus assay kit. All samples were inoculated onto solid media and 61 samples were inoculated in automated liquid culture also. The Line Probe Assay gave hundred percent interpretable results with 2.5% of the study population showing resistant pattern. Only 1% of the cases were primary multidrug resistant tuberculosis and 1.5% showed isoniazid monoresistance. S531L and C15T were the most common genetic mutations seen for rifampicin and isoniazid resistance, respectively. 40% had absent rpoB wild type 8 band indicating probable silent mutation after clinical correlation. The average turnaround time for Line Probe Assay was far less (3.8 days) as compared to solid and liquid cultures (35.6 days and 13.5 days, resp.).

No MeSH data available.


Related in: MedlinePlus