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Cytotoxicity and Antiproliferative Activity Assay of Clove Mistletoe (Dendrophthoe pentandra (L.) Miq.) Leaves Extracts.

Elsyana V, Bintang M, Priosoeryanto BP - Adv Pharmacol Sci (2016)

Bottom Line: Clove mistletoe was traditionally used for cancer treatment in Indonesia.The n-hexane fraction was then considered for further examination.Therefore, it was suggested that clove mistletoe had potential natural anticancer activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor 16680, Indonesia.

ABSTRACT
Clove mistletoe (Dendrophthoe pentandra (L.) Miq.) is a semiparasitic plant that belongs to Loranthaceae family. Clove mistletoe was traditionally used for cancer treatment in Indonesia. In the present study, we examined cytotoxicity of clove mistletoe leaves extracts against brine shrimps and conducted their antiproliferative activity on K562 (human chronic myelogenous leukemia) and MCM-B2 (canine benign mixed mammary) cancer cell lines in vitro. The tested samples were water extract, ethanol extract, ethanol fraction, ethyl acetate fraction, and n-hexane fraction. Cytotoxicity was screened using Brine Shrimp Lethality Test (BSLT). Antiproliferative activity was conducted using Trypan Blue Dye Method and cells were counted using haemocytometer. The results showed that n-hexane fraction exhibited significant cytotoxicity with LC50 value of 55.31 μg/mL. The n-hexane fraction was then considered for further examination. The n-hexane fraction of clove mistletoe could inhibit growth of K562 and MCM-B2 cancer cell lines in vitro. The inhibition activity of clove mistletoe n-hexane fraction at concentration of 125 μg/mL on K562 cancer cell lines was 38.69%, while on MCM-B2 it was 41.5%. Therefore, it was suggested that clove mistletoe had potential natural anticancer activity.

No MeSH data available.


Related in: MedlinePlus

Percentage of proliferation inhibition MCM-B2 cells after treatment with n-hexane fraction of clove mistletoe. The data is expressed as percentage of proliferation inhibition ± SEM, as compared to the negative control (100%). Level of significance is denoted as follows: ∗P < 0.05; ∗∗P < 0.01.
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fig2: Percentage of proliferation inhibition MCM-B2 cells after treatment with n-hexane fraction of clove mistletoe. The data is expressed as percentage of proliferation inhibition ± SEM, as compared to the negative control (100%). Level of significance is denoted as follows: ∗P < 0.05; ∗∗P < 0.01.

Mentions: The antiproliferative activity in vitro of n-hexane fraction was conducted with K562 (human chronic myelogenous leukemia) and MCM-B2 (canine benign mixed mammary) cancer cell lines. The K562 and MCM-B2 proliferation inhibitions after n-hexane fraction treatment were presented in Figures 1 and 2. K562 and MCM-B2 cancer cells decreased with the increase of the given tested concentrations. At the lowest tested concentration (25 μg/mL), n-hexane fraction could inhibit cell of both cancer cell lines.


Cytotoxicity and Antiproliferative Activity Assay of Clove Mistletoe (Dendrophthoe pentandra (L.) Miq.) Leaves Extracts.

Elsyana V, Bintang M, Priosoeryanto BP - Adv Pharmacol Sci (2016)

Percentage of proliferation inhibition MCM-B2 cells after treatment with n-hexane fraction of clove mistletoe. The data is expressed as percentage of proliferation inhibition ± SEM, as compared to the negative control (100%). Level of significance is denoted as follows: ∗P < 0.05; ∗∗P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4821920&req=5

fig2: Percentage of proliferation inhibition MCM-B2 cells after treatment with n-hexane fraction of clove mistletoe. The data is expressed as percentage of proliferation inhibition ± SEM, as compared to the negative control (100%). Level of significance is denoted as follows: ∗P < 0.05; ∗∗P < 0.01.
Mentions: The antiproliferative activity in vitro of n-hexane fraction was conducted with K562 (human chronic myelogenous leukemia) and MCM-B2 (canine benign mixed mammary) cancer cell lines. The K562 and MCM-B2 proliferation inhibitions after n-hexane fraction treatment were presented in Figures 1 and 2. K562 and MCM-B2 cancer cells decreased with the increase of the given tested concentrations. At the lowest tested concentration (25 μg/mL), n-hexane fraction could inhibit cell of both cancer cell lines.

Bottom Line: Clove mistletoe was traditionally used for cancer treatment in Indonesia.The n-hexane fraction was then considered for further examination.Therefore, it was suggested that clove mistletoe had potential natural anticancer activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor 16680, Indonesia.

ABSTRACT
Clove mistletoe (Dendrophthoe pentandra (L.) Miq.) is a semiparasitic plant that belongs to Loranthaceae family. Clove mistletoe was traditionally used for cancer treatment in Indonesia. In the present study, we examined cytotoxicity of clove mistletoe leaves extracts against brine shrimps and conducted their antiproliferative activity on K562 (human chronic myelogenous leukemia) and MCM-B2 (canine benign mixed mammary) cancer cell lines in vitro. The tested samples were water extract, ethanol extract, ethanol fraction, ethyl acetate fraction, and n-hexane fraction. Cytotoxicity was screened using Brine Shrimp Lethality Test (BSLT). Antiproliferative activity was conducted using Trypan Blue Dye Method and cells were counted using haemocytometer. The results showed that n-hexane fraction exhibited significant cytotoxicity with LC50 value of 55.31 μg/mL. The n-hexane fraction was then considered for further examination. The n-hexane fraction of clove mistletoe could inhibit growth of K562 and MCM-B2 cancer cell lines in vitro. The inhibition activity of clove mistletoe n-hexane fraction at concentration of 125 μg/mL on K562 cancer cell lines was 38.69%, while on MCM-B2 it was 41.5%. Therefore, it was suggested that clove mistletoe had potential natural anticancer activity.

No MeSH data available.


Related in: MedlinePlus