Limits...
Exogenous abscisic acid significantly affects proteome in tea plant (Camellia sinensis) exposed to drought stress.

Zhou L, Xu H, Mischke S, Meinhardt LW, Zhang D, Zhu X, Li X, Fang W - Hortic Res (2014)

Bottom Line: The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress.The upregulated proteins have roles in glycolysis and photosystem II stabilization.The results support the importance of the role that ABA plays in the tea plant during drought stress, by improving protein transport, carbon metabolism and expression of resistance proteins.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China.

ABSTRACT
Tea [Camellia sinensis (L.) O. Kuntze] is an important economic crop, and drought is the most important abiotic stress affecting yield and quality. Abscisic acid (ABA) is an important phytohormone responsible for activating drought resistance. Increased understanding of ABA effects on tea plant under drought stress is essential to develop drought-tolerant tea genotypes, along with crop management practices that can mitigate drought stress. The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress. Leaf protein patterns of tea plants under simulated drought stress [(polyethylene glycol (PEG)-treated] and exogenous ABA treatment were analyzed in a time-course experiment using two-dimensional electrophoresis (2-DE), followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Among the 72 protein spots identified by MALDI-TOF MS, 16 proteins were downregulated and two were upregulated by exogenous ABA. The upregulated proteins have roles in glycolysis and photosystem II stabilization. Twenty-one protein spots were responsive to drought stress and most participate in carbohydrate and nitrogen metabolism, control of reactive oxygen species (ROS), defense, signaling or nucleic acid metabolism. The combined treatments of exogenous ABA and drought showed upregulation of 10 protein spots at 12 h and upregulation of 11 proteins at 72 h after initiation of drought stress. The results support the importance of the role that ABA plays in the tea plant during drought stress, by improving protein transport, carbon metabolism and expression of resistance proteins.

No MeSH data available.


Related in: MedlinePlus

A 2-DE analysis of tea leaf proteins. (a) 2-DE gels of tea leaf subjected to distilled water (control); (b, c) 2-DE gels of tea leaf subjected to drought treatment for 12 h and 72 h. This is a representative figure from three biological replicas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4816387&req=5

fig5: A 2-DE analysis of tea leaf proteins. (a) 2-DE gels of tea leaf subjected to distilled water (control); (b, c) 2-DE gels of tea leaf subjected to drought treatment for 12 h and 72 h. This is a representative figure from three biological replicas.

Mentions: In order to show the effects of exogenous ABA treatment on these proteins induced by drought stress, the leaf protein pattern in tea plants pre-treated with exogenous ABA was also analyzed using 2-DE. Thirty-three differentially expressed proteins were selected for categorizing (Table 3). Seven of these proteins (spots 6, 7, 28 and 30–33) were predicted proteins, or unknown or unnamed proteins; 19 were in the Rubisco family; and another seven were proteins responding to PEG stress. The latter proteins included apolipoprotein, chloroplast phosphoribulokinase, cytochrome b6-f complex iron-sulfur subunit 2, fructose-bisphosphate aldolase, high molecular weight heat shock protein (Hsp), S-adenosylmethionine synthase 3 and short-chain dehydrogenase/reductase. These proteins play important roles in many life activities of plant, such as transporter activity, photosynthesis, glycolysis, redox reactions and amino-acid biosynthesis. The expression pattern of these proteins in leaves of tea plant pre-treated with exogenous ABA and single drought treatment (Figure 5) suggests that 23 proteins (spots 1, 2, 4, 5, 7, 9–15, 17, 19–21, 23, 25–29 and 31) were upregulated and 10 spots (spot 3, 6, 8, 16, 18, 22, 24, 30, 32 and 33) were downregulated at 12 h; 11 proteins (spots 1, 4, 5, 11, 19, 20, 22, 24, 27, 29 and 30) were upregulated at 72 h; and some proteins were downregulated compared with 0 h (Figures 5 and 6). Apolipoprotein D, chloroplast phosphoribulokinase, fructose-bisphosphate aldolase, high molecular weight Hsp, protein IN2-1 homolog B-like, short-chain dehydrogenase/reductase and most of the Rubisco proteins were upregulated at 12 h in tea plants that had been pre-treated with ABA.


Exogenous abscisic acid significantly affects proteome in tea plant (Camellia sinensis) exposed to drought stress.

Zhou L, Xu H, Mischke S, Meinhardt LW, Zhang D, Zhu X, Li X, Fang W - Hortic Res (2014)

A 2-DE analysis of tea leaf proteins. (a) 2-DE gels of tea leaf subjected to distilled water (control); (b, c) 2-DE gels of tea leaf subjected to drought treatment for 12 h and 72 h. This is a representative figure from three biological replicas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4816387&req=5

fig5: A 2-DE analysis of tea leaf proteins. (a) 2-DE gels of tea leaf subjected to distilled water (control); (b, c) 2-DE gels of tea leaf subjected to drought treatment for 12 h and 72 h. This is a representative figure from three biological replicas.
Mentions: In order to show the effects of exogenous ABA treatment on these proteins induced by drought stress, the leaf protein pattern in tea plants pre-treated with exogenous ABA was also analyzed using 2-DE. Thirty-three differentially expressed proteins were selected for categorizing (Table 3). Seven of these proteins (spots 6, 7, 28 and 30–33) were predicted proteins, or unknown or unnamed proteins; 19 were in the Rubisco family; and another seven were proteins responding to PEG stress. The latter proteins included apolipoprotein, chloroplast phosphoribulokinase, cytochrome b6-f complex iron-sulfur subunit 2, fructose-bisphosphate aldolase, high molecular weight heat shock protein (Hsp), S-adenosylmethionine synthase 3 and short-chain dehydrogenase/reductase. These proteins play important roles in many life activities of plant, such as transporter activity, photosynthesis, glycolysis, redox reactions and amino-acid biosynthesis. The expression pattern of these proteins in leaves of tea plant pre-treated with exogenous ABA and single drought treatment (Figure 5) suggests that 23 proteins (spots 1, 2, 4, 5, 7, 9–15, 17, 19–21, 23, 25–29 and 31) were upregulated and 10 spots (spot 3, 6, 8, 16, 18, 22, 24, 30, 32 and 33) were downregulated at 12 h; 11 proteins (spots 1, 4, 5, 11, 19, 20, 22, 24, 27, 29 and 30) were upregulated at 72 h; and some proteins were downregulated compared with 0 h (Figures 5 and 6). Apolipoprotein D, chloroplast phosphoribulokinase, fructose-bisphosphate aldolase, high molecular weight Hsp, protein IN2-1 homolog B-like, short-chain dehydrogenase/reductase and most of the Rubisco proteins were upregulated at 12 h in tea plants that had been pre-treated with ABA.

Bottom Line: The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress.The upregulated proteins have roles in glycolysis and photosystem II stabilization.The results support the importance of the role that ABA plays in the tea plant during drought stress, by improving protein transport, carbon metabolism and expression of resistance proteins.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China.

ABSTRACT
Tea [Camellia sinensis (L.) O. Kuntze] is an important economic crop, and drought is the most important abiotic stress affecting yield and quality. Abscisic acid (ABA) is an important phytohormone responsible for activating drought resistance. Increased understanding of ABA effects on tea plant under drought stress is essential to develop drought-tolerant tea genotypes, along with crop management practices that can mitigate drought stress. The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress. Leaf protein patterns of tea plants under simulated drought stress [(polyethylene glycol (PEG)-treated] and exogenous ABA treatment were analyzed in a time-course experiment using two-dimensional electrophoresis (2-DE), followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Among the 72 protein spots identified by MALDI-TOF MS, 16 proteins were downregulated and two were upregulated by exogenous ABA. The upregulated proteins have roles in glycolysis and photosystem II stabilization. Twenty-one protein spots were responsive to drought stress and most participate in carbohydrate and nitrogen metabolism, control of reactive oxygen species (ROS), defense, signaling or nucleic acid metabolism. The combined treatments of exogenous ABA and drought showed upregulation of 10 protein spots at 12 h and upregulation of 11 proteins at 72 h after initiation of drought stress. The results support the importance of the role that ABA plays in the tea plant during drought stress, by improving protein transport, carbon metabolism and expression of resistance proteins.

No MeSH data available.


Related in: MedlinePlus