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Exogenous abscisic acid significantly affects proteome in tea plant (Camellia sinensis) exposed to drought stress.

Zhou L, Xu H, Mischke S, Meinhardt LW, Zhang D, Zhu X, Li X, Fang W - Hortic Res (2014)

Bottom Line: The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress.The upregulated proteins have roles in glycolysis and photosystem II stabilization.The results support the importance of the role that ABA plays in the tea plant during drought stress, by improving protein transport, carbon metabolism and expression of resistance proteins.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China.

ABSTRACT
Tea [Camellia sinensis (L.) O. Kuntze] is an important economic crop, and drought is the most important abiotic stress affecting yield and quality. Abscisic acid (ABA) is an important phytohormone responsible for activating drought resistance. Increased understanding of ABA effects on tea plant under drought stress is essential to develop drought-tolerant tea genotypes, along with crop management practices that can mitigate drought stress. The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress. Leaf protein patterns of tea plants under simulated drought stress [(polyethylene glycol (PEG)-treated] and exogenous ABA treatment were analyzed in a time-course experiment using two-dimensional electrophoresis (2-DE), followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Among the 72 protein spots identified by MALDI-TOF MS, 16 proteins were downregulated and two were upregulated by exogenous ABA. The upregulated proteins have roles in glycolysis and photosystem II stabilization. Twenty-one protein spots were responsive to drought stress and most participate in carbohydrate and nitrogen metabolism, control of reactive oxygen species (ROS), defense, signaling or nucleic acid metabolism. The combined treatments of exogenous ABA and drought showed upregulation of 10 protein spots at 12 h and upregulation of 11 proteins at 72 h after initiation of drought stress. The results support the importance of the role that ABA plays in the tea plant during drought stress, by improving protein transport, carbon metabolism and expression of resistance proteins.

No MeSH data available.


Related in: MedlinePlus

A 2-DE analysis of tea leaf proteins. (a) 2-DE gels of tea leaf subjected to 50 mg L−1 ABA; (b, c) 2-DE gels of tea leaf subjected to drought treatment for 12 h and 72 h after pre-treated with 50 mg L−1 ABA. This is a representative figure from three biological replicas.
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fig3: A 2-DE analysis of tea leaf proteins. (a) 2-DE gels of tea leaf subjected to 50 mg L−1 ABA; (b, c) 2-DE gels of tea leaf subjected to drought treatment for 12 h and 72 h after pre-treated with 50 mg L−1 ABA. This is a representative figure from three biological replicas.

Mentions: The proteome of tea plant leaves in response to drought stress was resolved by 2-DE, using triplicate gels. Representative gels are illustrated in Figure 3, and the global pattern of proteins remained largely unaltered. However, the expression levels of 21 proteins were changed at least 2.0-fold in one or both of the drought treatment stages (Figure 4). Besides the unknown and predicted proteins (spots 14, 18, 19, 20 and 21), other identified proteins were classified into several functional categories including regulatory proteins, energy-related proteins, redox homeostasis-related proteins, etc. (Table 2). The largest functional protein was involved in carbohydrate metabolic processes and nitrogen metabolic processes, which were greatly affected by drought stress. Comparison of the identified proteins between drought-affected and control samples showed all spots except 12, 20 and 21 were significantly downregulated in tea plant for the 12 h samples (Figure 4). Compared with the controls, five protein spots (spots 9, 12, 17, 18 and 19) were upregulated, and 16 spots (spots 1–8, 10, 11, 13–16, 20 and 21) were downregulated (Figure 4). Spots 2 and 20 were present in abundance in the control leaves, but undetected in PEG-treated leaves at 72 h, suggesting that PEG-induced alterations of membrane proteins in tea plant leaves included both quantitative (increased or decreased) and qualitative changes in protein spots. One of inevitable consequences of water stress is that ROS accumulation is enhanced and distributed in different cellular compartments, e.g., chloroplasts, the peroxisomes and the mitochondria. Treatment with 10% PEG-6000 for 12 h and 72 h, damaged the photosynthetic and antioxidant enzyme systems of tea plants.


Exogenous abscisic acid significantly affects proteome in tea plant (Camellia sinensis) exposed to drought stress.

Zhou L, Xu H, Mischke S, Meinhardt LW, Zhang D, Zhu X, Li X, Fang W - Hortic Res (2014)

A 2-DE analysis of tea leaf proteins. (a) 2-DE gels of tea leaf subjected to 50 mg L−1 ABA; (b, c) 2-DE gels of tea leaf subjected to drought treatment for 12 h and 72 h after pre-treated with 50 mg L−1 ABA. This is a representative figure from three biological replicas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4816387&req=5

fig3: A 2-DE analysis of tea leaf proteins. (a) 2-DE gels of tea leaf subjected to 50 mg L−1 ABA; (b, c) 2-DE gels of tea leaf subjected to drought treatment for 12 h and 72 h after pre-treated with 50 mg L−1 ABA. This is a representative figure from three biological replicas.
Mentions: The proteome of tea plant leaves in response to drought stress was resolved by 2-DE, using triplicate gels. Representative gels are illustrated in Figure 3, and the global pattern of proteins remained largely unaltered. However, the expression levels of 21 proteins were changed at least 2.0-fold in one or both of the drought treatment stages (Figure 4). Besides the unknown and predicted proteins (spots 14, 18, 19, 20 and 21), other identified proteins were classified into several functional categories including regulatory proteins, energy-related proteins, redox homeostasis-related proteins, etc. (Table 2). The largest functional protein was involved in carbohydrate metabolic processes and nitrogen metabolic processes, which were greatly affected by drought stress. Comparison of the identified proteins between drought-affected and control samples showed all spots except 12, 20 and 21 were significantly downregulated in tea plant for the 12 h samples (Figure 4). Compared with the controls, five protein spots (spots 9, 12, 17, 18 and 19) were upregulated, and 16 spots (spots 1–8, 10, 11, 13–16, 20 and 21) were downregulated (Figure 4). Spots 2 and 20 were present in abundance in the control leaves, but undetected in PEG-treated leaves at 72 h, suggesting that PEG-induced alterations of membrane proteins in tea plant leaves included both quantitative (increased or decreased) and qualitative changes in protein spots. One of inevitable consequences of water stress is that ROS accumulation is enhanced and distributed in different cellular compartments, e.g., chloroplasts, the peroxisomes and the mitochondria. Treatment with 10% PEG-6000 for 12 h and 72 h, damaged the photosynthetic and antioxidant enzyme systems of tea plants.

Bottom Line: The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress.The upregulated proteins have roles in glycolysis and photosystem II stabilization.The results support the importance of the role that ABA plays in the tea plant during drought stress, by improving protein transport, carbon metabolism and expression of resistance proteins.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China.

ABSTRACT
Tea [Camellia sinensis (L.) O. Kuntze] is an important economic crop, and drought is the most important abiotic stress affecting yield and quality. Abscisic acid (ABA) is an important phytohormone responsible for activating drought resistance. Increased understanding of ABA effects on tea plant under drought stress is essential to develop drought-tolerant tea genotypes, along with crop management practices that can mitigate drought stress. The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress. Leaf protein patterns of tea plants under simulated drought stress [(polyethylene glycol (PEG)-treated] and exogenous ABA treatment were analyzed in a time-course experiment using two-dimensional electrophoresis (2-DE), followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Among the 72 protein spots identified by MALDI-TOF MS, 16 proteins were downregulated and two were upregulated by exogenous ABA. The upregulated proteins have roles in glycolysis and photosystem II stabilization. Twenty-one protein spots were responsive to drought stress and most participate in carbohydrate and nitrogen metabolism, control of reactive oxygen species (ROS), defense, signaling or nucleic acid metabolism. The combined treatments of exogenous ABA and drought showed upregulation of 10 protein spots at 12 h and upregulation of 11 proteins at 72 h after initiation of drought stress. The results support the importance of the role that ABA plays in the tea plant during drought stress, by improving protein transport, carbon metabolism and expression of resistance proteins.

No MeSH data available.


Related in: MedlinePlus