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Direct growth inhibition assay of total airborne fungi with application of biocide-treated malt extract agar.

Er CM, Sunar NM, Leman AM, Othman N - MethodsX (2015)

Bottom Line: Indoor air pollution by airborne fungi has risen to become a common issue all over the world and it is hazardous to indoor occupants' health as it is associated with a series of respiratory-related and skin-related diseases.Selected bioactive compounds from the food industry have been suggested to be effective against individual fungus isolated from indoor environment.However, the techniques used to evaluate these compounds were lengthy and unsuitable against total airborne fungi.

View Article: PubMed Central - PubMed

Affiliation: Department of Water and Environmental Engineering (DWEE), Faculty of Civil and Environmental Engineering (FKAAS), Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia.

ABSTRACT
Indoor air pollution by airborne fungi has risen to become a common issue all over the world and it is hazardous to indoor occupants' health as it is associated with a series of respiratory-related and skin-related diseases. Selected bioactive compounds from the food industry have been suggested to be effective against individual fungus isolated from indoor environment. However, the techniques used to evaluate these compounds were lengthy and unsuitable against total airborne fungi. Therefore, this paper describes an assay to assess the effectiveness of a bioactive compound to inhibit growth of total airborne fungi.•A combination and modification of previous methods and the NIOSH Manual Analytical Standard Method (NMAM 0800) is proposed.•This method concurrently samples the total airborne fungi and evaluates the ability of bioactive compounds (potassium sorbate in this paper), as a biocide, to treat these indoor airborne fungi.•The current method shortens the time of evaluation from 30 days to only 5 days and employs the counting of colony forming units (CFUs) to ease the measurement of the growth of fungi.

No MeSH data available.


The comparison of the total airborne fungi found on(a) untreated MEA (control) and (b) biocide-treated MEA at a testing site in abuilding after 5 days of incubation at 37 °C.
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fig0005: The comparison of the total airborne fungi found on(a) untreated MEA (control) and (b) biocide-treated MEA at a testing site in abuilding after 5 days of incubation at 37 °C.

Mentions: The enumeration of the samples is indicated by colonyforming unit (CFU) analysis [13]. The counting process was done by mounting the agarplate on digital colony counter and the colonies were counted manually(Fig.1). The total number ofthe fungi colonies formed on the agar plate was then divided with the totalvolume of air drew by the sampler. The calculation is as follows[14]:(1)CFU/m3=1000 (Number of colony)Sampling time (min)×Flow rate (L/min)


Direct growth inhibition assay of total airborne fungi with application of biocide-treated malt extract agar.

Er CM, Sunar NM, Leman AM, Othman N - MethodsX (2015)

The comparison of the total airborne fungi found on(a) untreated MEA (control) and (b) biocide-treated MEA at a testing site in abuilding after 5 days of incubation at 37 °C.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4816262&req=5

fig0005: The comparison of the total airborne fungi found on(a) untreated MEA (control) and (b) biocide-treated MEA at a testing site in abuilding after 5 days of incubation at 37 °C.
Mentions: The enumeration of the samples is indicated by colonyforming unit (CFU) analysis [13]. The counting process was done by mounting the agarplate on digital colony counter and the colonies were counted manually(Fig.1). The total number ofthe fungi colonies formed on the agar plate was then divided with the totalvolume of air drew by the sampler. The calculation is as follows[14]:(1)CFU/m3=1000 (Number of colony)Sampling time (min)×Flow rate (L/min)

Bottom Line: Indoor air pollution by airborne fungi has risen to become a common issue all over the world and it is hazardous to indoor occupants' health as it is associated with a series of respiratory-related and skin-related diseases.Selected bioactive compounds from the food industry have been suggested to be effective against individual fungus isolated from indoor environment.However, the techniques used to evaluate these compounds were lengthy and unsuitable against total airborne fungi.

View Article: PubMed Central - PubMed

Affiliation: Department of Water and Environmental Engineering (DWEE), Faculty of Civil and Environmental Engineering (FKAAS), Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia.

ABSTRACT
Indoor air pollution by airborne fungi has risen to become a common issue all over the world and it is hazardous to indoor occupants' health as it is associated with a series of respiratory-related and skin-related diseases. Selected bioactive compounds from the food industry have been suggested to be effective against individual fungus isolated from indoor environment. However, the techniques used to evaluate these compounds were lengthy and unsuitable against total airborne fungi. Therefore, this paper describes an assay to assess the effectiveness of a bioactive compound to inhibit growth of total airborne fungi.•A combination and modification of previous methods and the NIOSH Manual Analytical Standard Method (NMAM 0800) is proposed.•This method concurrently samples the total airborne fungi and evaluates the ability of bioactive compounds (potassium sorbate in this paper), as a biocide, to treat these indoor airborne fungi.•The current method shortens the time of evaluation from 30 days to only 5 days and employs the counting of colony forming units (CFUs) to ease the measurement of the growth of fungi.

No MeSH data available.