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Insecticide susceptibility of natural populations of Anopheles coluzzii and Anopheles gambiae (sensu stricto) from Okyereko irrigation site, Ghana, West Africa.

Chabi J, Baidoo PK, Datsomor AK, Okyere D, Ablorde A, Iddrisu A, Wilson MD, Dadzie SK, Jamet HP, Diclaro JW - Parasit Vectors (2016)

Bottom Line: The An. gambiae (s.l.) populations from Okyereko are highly resistant to DDT and pyrethroid insecticides, with possible involvement of metabolic mechanisms including the elevation of P450 and GST enzyme as well as P-gp activity.The population was mostly composed of An. coluzzii specimens (more than 96 %) with kdr and ace-1 frequencies of 0.9 and 0.2 %, respectively.This study brings additional information on insecticide resistance and the characterization of An. gambiae (s.l.) mosquitoes from Okyereko, which can be helpful in decision making for vector control programmes in the region.

View Article: PubMed Central - PubMed

Affiliation: Vestergaard-NMIMR Vector Labs (VNVL), Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana. chabijoseph@yahoo.fr.

ABSTRACT

Background: The increasing spread of insecticide resistance in malaria vectors has been well documented across sub-Saharan Africa countries. The influence of irrigation on increasing vector resistance is poorly understood, and is critical to successful and ethical implementation of food security policies. This study investigated the insecticide resistance status of An. gambiae (s.l.) mosquitoes collected from the irrigated rice area of Okyereko, a village containing about 42 hectares of irrigated field within an irrigation project plan in the Central Region of Ghana. Large amounts of insecticides, herbicides and fertilizers are commonly used in the area to boost the annual production of the rice.

Methods: Mosquito larvae were collected and adults were assayed from the F1 progeny. The resistance status, allele and genotype were characterized using WHO susceptibility testing and PCR methods respectively.

Results: The An. gambiae (s.l.) populations from Okyereko are highly resistant to DDT and pyrethroid insecticides, with possible involvement of metabolic mechanisms including the elevation of P450 and GST enzyme as well as P-gp activity. The population was mostly composed of An. coluzzii specimens (more than 96 %) with kdr and ace-1 frequencies of 0.9 and 0.2 %, respectively.

Conclusion: This study brings additional information on insecticide resistance and the characterization of An. gambiae (s.l.) mosquitoes from Okyereko, which can be helpful in decision making for vector control programmes in the region.

No MeSH data available.


Related in: MedlinePlus

Anopheles gambiae (s.l.) female from Okyereko knock-down rate after exposure time and 24 h delayed mortality observed using organophosphate and carbamate insecticides. Error bars represent 95 % confidence interval (CI)
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Fig2: Anopheles gambiae (s.l.) female from Okyereko knock-down rate after exposure time and 24 h delayed mortality observed using organophosphate and carbamate insecticides. Error bars represent 95 % confidence interval (CI)

Mentions: A total of 157 female An. gambiae (s.l.) from Okyereko were assayed for deltamethrin, 132 for permethrin and an average of 100 mosquitoes for all other insecticides. Mortality observed with DDT and pyrethroid insecticides was very low, ranging from 0 % with DDT to 18.4 and 34.1 % with permethrin and deltamethrin respectively. Higher mortalities were observed with the organophosphates with 86 % mortality for fenitrothion and 100 % with malathion. The observed mortalities using carbamates were 64 % for bendiocarb and 57 % for propoxur (Figs. 2 and 3).Fig. 2


Insecticide susceptibility of natural populations of Anopheles coluzzii and Anopheles gambiae (sensu stricto) from Okyereko irrigation site, Ghana, West Africa.

Chabi J, Baidoo PK, Datsomor AK, Okyere D, Ablorde A, Iddrisu A, Wilson MD, Dadzie SK, Jamet HP, Diclaro JW - Parasit Vectors (2016)

Anopheles gambiae (s.l.) female from Okyereko knock-down rate after exposure time and 24 h delayed mortality observed using organophosphate and carbamate insecticides. Error bars represent 95 % confidence interval (CI)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4815066&req=5

Fig2: Anopheles gambiae (s.l.) female from Okyereko knock-down rate after exposure time and 24 h delayed mortality observed using organophosphate and carbamate insecticides. Error bars represent 95 % confidence interval (CI)
Mentions: A total of 157 female An. gambiae (s.l.) from Okyereko were assayed for deltamethrin, 132 for permethrin and an average of 100 mosquitoes for all other insecticides. Mortality observed with DDT and pyrethroid insecticides was very low, ranging from 0 % with DDT to 18.4 and 34.1 % with permethrin and deltamethrin respectively. Higher mortalities were observed with the organophosphates with 86 % mortality for fenitrothion and 100 % with malathion. The observed mortalities using carbamates were 64 % for bendiocarb and 57 % for propoxur (Figs. 2 and 3).Fig. 2

Bottom Line: The An. gambiae (s.l.) populations from Okyereko are highly resistant to DDT and pyrethroid insecticides, with possible involvement of metabolic mechanisms including the elevation of P450 and GST enzyme as well as P-gp activity.The population was mostly composed of An. coluzzii specimens (more than 96 %) with kdr and ace-1 frequencies of 0.9 and 0.2 %, respectively.This study brings additional information on insecticide resistance and the characterization of An. gambiae (s.l.) mosquitoes from Okyereko, which can be helpful in decision making for vector control programmes in the region.

View Article: PubMed Central - PubMed

Affiliation: Vestergaard-NMIMR Vector Labs (VNVL), Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana. chabijoseph@yahoo.fr.

ABSTRACT

Background: The increasing spread of insecticide resistance in malaria vectors has been well documented across sub-Saharan Africa countries. The influence of irrigation on increasing vector resistance is poorly understood, and is critical to successful and ethical implementation of food security policies. This study investigated the insecticide resistance status of An. gambiae (s.l.) mosquitoes collected from the irrigated rice area of Okyereko, a village containing about 42 hectares of irrigated field within an irrigation project plan in the Central Region of Ghana. Large amounts of insecticides, herbicides and fertilizers are commonly used in the area to boost the annual production of the rice.

Methods: Mosquito larvae were collected and adults were assayed from the F1 progeny. The resistance status, allele and genotype were characterized using WHO susceptibility testing and PCR methods respectively.

Results: The An. gambiae (s.l.) populations from Okyereko are highly resistant to DDT and pyrethroid insecticides, with possible involvement of metabolic mechanisms including the elevation of P450 and GST enzyme as well as P-gp activity. The population was mostly composed of An. coluzzii specimens (more than 96 %) with kdr and ace-1 frequencies of 0.9 and 0.2 %, respectively.

Conclusion: This study brings additional information on insecticide resistance and the characterization of An. gambiae (s.l.) mosquitoes from Okyereko, which can be helpful in decision making for vector control programmes in the region.

No MeSH data available.


Related in: MedlinePlus