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Analysis of effects of a new environmental pollutant, bisphenol A, on antioxidant systems in soybean roots at different growth stages.

Zhang J, Li X, Zhou L, Wang L, Zhou Q, Huang X - Sci Rep (2016)

Bottom Line: Therefore, in this work, BPA's effects in soybean roots at different growth stages were investigated by determining the reactive oxygen species levels, membrane lipid fatty acid composition, membrane lipid peroxidation, and antioxidant systems.The results showed that low-dose BPA exposure slightly caused membrane lipid peroxidation but didn't activate antioxidant systems at the seedling stage, and this exposure did not affect above process at other growth stages; high-dose BPA increased reactive oxygen species levels and then caused membrane lipid peroxidation at all growth stages although it activated antioxidant systems, and these effects were weaker with prolonging the growth stages.Taken together, the effects of BPA on antioxidant systems in soybean roots were associated with BPA exposure dose and soybean growth stage.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Food Science and Technology, School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China.

ABSTRACT
Bisphenol A (BPA) is an important industrial raw material. Because of its widespread use and increasing release into environment, BPA has become a new environmental pollutant. Previous studies about BPA's effects in plants focus on a certain growth stage. However, the plant's response to pollutants varies at different growth stages. Therefore, in this work, BPA's effects in soybean roots at different growth stages were investigated by determining the reactive oxygen species levels, membrane lipid fatty acid composition, membrane lipid peroxidation, and antioxidant systems. The results showed that low-dose BPA exposure slightly caused membrane lipid peroxidation but didn't activate antioxidant systems at the seedling stage, and this exposure did not affect above process at other growth stages; high-dose BPA increased reactive oxygen species levels and then caused membrane lipid peroxidation at all growth stages although it activated antioxidant systems, and these effects were weaker with prolonging the growth stages. The recovery degree after withdrawal of BPA exposure was negatively related to BPA dose, but was positively related to growth stage. Taken together, the effects of BPA on antioxidant systems in soybean roots were associated with BPA exposure dose and soybean growth stage.

No MeSH data available.


Related in: MedlinePlus

Effects of BPA on the contents of hydrogen peroxide (H2O2), superoxide anion (O2−), malondialdehyde (MDA) and membrane permeability (E%) in soybean roots at different growth stages following BPA exposure and withdrawal of BPA exposure.Significant differences at p < 0.05 are denoted with different letters at different growth stages.
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f1: Effects of BPA on the contents of hydrogen peroxide (H2O2), superoxide anion (O2−), malondialdehyde (MDA) and membrane permeability (E%) in soybean roots at different growth stages following BPA exposure and withdrawal of BPA exposure.Significant differences at p < 0.05 are denoted with different letters at different growth stages.

Mentions: Figure 1 shows the effects of BPA on ROS levels and membrane lipid peroxidation in soybean roots at different growth stages. Compared to the control, after 7 d of 1.5 mg·L−1 BPA exposure, the H2O2 content at the seedling stage increased (p < 0.05), but the O2− and malondialdehyde (MDA) contents and membrane permeability did not change (p > 0.05), and those indices at both flowering and podding stages and seed-filling stages did not change (p > 0.05). With an increasing BPA dose (6.0 and 12.0 mg·L−1), the O2− content, MDA content, and membrane permeability significantly increased (p < 0.05) at the three growth stages. Moreover, the increase degree of the O2− content and membrane permeability were positively related to the BPA dose, but negatively related to the growth stage. Moreover, the O2− content, MDA content, and membrane permeability at the three growth stages did not change (p > 0.05) after withdrawal of 1.5 mg·L−1 BPA exposure, but increased (p < 0.05) after withdrawal of 6.0 and 12.0 mg·L−1 BPA exposure.


Analysis of effects of a new environmental pollutant, bisphenol A, on antioxidant systems in soybean roots at different growth stages.

Zhang J, Li X, Zhou L, Wang L, Zhou Q, Huang X - Sci Rep (2016)

Effects of BPA on the contents of hydrogen peroxide (H2O2), superoxide anion (O2−), malondialdehyde (MDA) and membrane permeability (E%) in soybean roots at different growth stages following BPA exposure and withdrawal of BPA exposure.Significant differences at p < 0.05 are denoted with different letters at different growth stages.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4815016&req=5

f1: Effects of BPA on the contents of hydrogen peroxide (H2O2), superoxide anion (O2−), malondialdehyde (MDA) and membrane permeability (E%) in soybean roots at different growth stages following BPA exposure and withdrawal of BPA exposure.Significant differences at p < 0.05 are denoted with different letters at different growth stages.
Mentions: Figure 1 shows the effects of BPA on ROS levels and membrane lipid peroxidation in soybean roots at different growth stages. Compared to the control, after 7 d of 1.5 mg·L−1 BPA exposure, the H2O2 content at the seedling stage increased (p < 0.05), but the O2− and malondialdehyde (MDA) contents and membrane permeability did not change (p > 0.05), and those indices at both flowering and podding stages and seed-filling stages did not change (p > 0.05). With an increasing BPA dose (6.0 and 12.0 mg·L−1), the O2− content, MDA content, and membrane permeability significantly increased (p < 0.05) at the three growth stages. Moreover, the increase degree of the O2− content and membrane permeability were positively related to the BPA dose, but negatively related to the growth stage. Moreover, the O2− content, MDA content, and membrane permeability at the three growth stages did not change (p > 0.05) after withdrawal of 1.5 mg·L−1 BPA exposure, but increased (p < 0.05) after withdrawal of 6.0 and 12.0 mg·L−1 BPA exposure.

Bottom Line: Therefore, in this work, BPA's effects in soybean roots at different growth stages were investigated by determining the reactive oxygen species levels, membrane lipid fatty acid composition, membrane lipid peroxidation, and antioxidant systems.The results showed that low-dose BPA exposure slightly caused membrane lipid peroxidation but didn't activate antioxidant systems at the seedling stage, and this exposure did not affect above process at other growth stages; high-dose BPA increased reactive oxygen species levels and then caused membrane lipid peroxidation at all growth stages although it activated antioxidant systems, and these effects were weaker with prolonging the growth stages.Taken together, the effects of BPA on antioxidant systems in soybean roots were associated with BPA exposure dose and soybean growth stage.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Food Science and Technology, School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China.

ABSTRACT
Bisphenol A (BPA) is an important industrial raw material. Because of its widespread use and increasing release into environment, BPA has become a new environmental pollutant. Previous studies about BPA's effects in plants focus on a certain growth stage. However, the plant's response to pollutants varies at different growth stages. Therefore, in this work, BPA's effects in soybean roots at different growth stages were investigated by determining the reactive oxygen species levels, membrane lipid fatty acid composition, membrane lipid peroxidation, and antioxidant systems. The results showed that low-dose BPA exposure slightly caused membrane lipid peroxidation but didn't activate antioxidant systems at the seedling stage, and this exposure did not affect above process at other growth stages; high-dose BPA increased reactive oxygen species levels and then caused membrane lipid peroxidation at all growth stages although it activated antioxidant systems, and these effects were weaker with prolonging the growth stages. The recovery degree after withdrawal of BPA exposure was negatively related to BPA dose, but was positively related to growth stage. Taken together, the effects of BPA on antioxidant systems in soybean roots were associated with BPA exposure dose and soybean growth stage.

No MeSH data available.


Related in: MedlinePlus