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Advanced Behavioral Analyses Show that the Presence of Food Causes Subtle Changes in C. elegans Movement.

Angstman NB, Frank HG, Schmitz C - Front Behav Neurosci (2016)

Bottom Line: In all seven investigated parameters, statistically significant differences were found in worm behavior between those moving on NGM-agar plates with an E. coli lawn and NGM-agar plates with no lawn.Furthermore, multiple test groups showed differences in interaction between variables as the parameters that significantly correlated statistically with speed of locomotion varied.The need to account for a nested design while performing statistical analyses in similar studies is also demonstrated.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroanatomy, Ludwig-Maximilians University of Munich Munich, Germany.

ABSTRACT
As a widely used and studied model organism, Caenorhabditis elegans worms offer the ability to investigate implications of behavioral change. Although, investigation of C. elegans behavioral traits has been shown, analysis is often narrowed down to measurements based off a single point, and thus cannot pick up on subtle behavioral and morphological changes. In the present study videos were captured of four different C. elegans strains grown in liquid cultures and transferred to NGM-agar plates with an E. coli lawn or with no lawn. Using an advanced software, WormLab, the full skeleton and outline of worms were tracked to determine whether the presence of food affects behavioral traits. In all seven investigated parameters, statistically significant differences were found in worm behavior between those moving on NGM-agar plates with an E. coli lawn and NGM-agar plates with no lawn. Furthermore, multiple test groups showed differences in interaction between variables as the parameters that significantly correlated statistically with speed of locomotion varied. In the present study, we demonstrate the validity of a model to analyze C. elegans behavior beyond simple speed of locomotion. The need to account for a nested design while performing statistical analyses in similar studies is also demonstrated. With extended analyses, C. elegans behavioral change can be investigated with greater sensitivity, which could have wide utility in fields such as, but not limited to, toxicology, drug discovery, and RNAi screening.

No MeSH data available.


Related in: MedlinePlus

Speed of locomotion of N2 (wild type) C. elegans on NGM agar plates with an E. coli lawn and on plates with no lawn. Groups A1–A10 and B1–B10 show individual data (green dots) and mean ± standard deviation (red lines) of speed of locomotion of N2 worms on 10 plates with an E. coli lawn (Groups A1–A10) and on 10 plates with no lawn (Groups B1–B10). Groups A and B show Tukey boxplots of the speed of locomotion of all worms on plates with an E. coli lawn (Group A) and of all worms on plates with no lawn (Group B). Nested ANOVA showed a statistically significant difference between worms on plates with an E. coli lawn and worms on plates with no lawn (p = 0.049) as well as a statistically significant effect among plates (p = 0.001; see also Table 2).
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Figure 3: Speed of locomotion of N2 (wild type) C. elegans on NGM agar plates with an E. coli lawn and on plates with no lawn. Groups A1–A10 and B1–B10 show individual data (green dots) and mean ± standard deviation (red lines) of speed of locomotion of N2 worms on 10 plates with an E. coli lawn (Groups A1–A10) and on 10 plates with no lawn (Groups B1–B10). Groups A and B show Tukey boxplots of the speed of locomotion of all worms on plates with an E. coli lawn (Group A) and of all worms on plates with no lawn (Group B). Nested ANOVA showed a statistically significant difference between worms on plates with an E. coli lawn and worms on plates with no lawn (p = 0.049) as well as a statistically significant effect among plates (p = 0.001; see also Table 2).

Mentions: In all experiments carried out, worms showed substantial interindividual variation in all investigated parameters. For example, speed of locomotion of N2 worms on plates with an E. coli lawn varied between 5.5 and 221 μm/s, and on plates with no lawn between 4.0 and 255 μm/s (Figure 3). Besides this, mean values of all investigated parameters showed considerable inter-plate variability. For example, mean speed of locomotion of N2 worms on plates with an E. coli lawn varied between 106 and 155 μm/s among plates, and on plates with no lawn between 112 and 186 μm/s among plates (Figure 3).


Advanced Behavioral Analyses Show that the Presence of Food Causes Subtle Changes in C. elegans Movement.

Angstman NB, Frank HG, Schmitz C - Front Behav Neurosci (2016)

Speed of locomotion of N2 (wild type) C. elegans on NGM agar plates with an E. coli lawn and on plates with no lawn. Groups A1–A10 and B1–B10 show individual data (green dots) and mean ± standard deviation (red lines) of speed of locomotion of N2 worms on 10 plates with an E. coli lawn (Groups A1–A10) and on 10 plates with no lawn (Groups B1–B10). Groups A and B show Tukey boxplots of the speed of locomotion of all worms on plates with an E. coli lawn (Group A) and of all worms on plates with no lawn (Group B). Nested ANOVA showed a statistically significant difference between worms on plates with an E. coli lawn and worms on plates with no lawn (p = 0.049) as well as a statistically significant effect among plates (p = 0.001; see also Table 2).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814519&req=5

Figure 3: Speed of locomotion of N2 (wild type) C. elegans on NGM agar plates with an E. coli lawn and on plates with no lawn. Groups A1–A10 and B1–B10 show individual data (green dots) and mean ± standard deviation (red lines) of speed of locomotion of N2 worms on 10 plates with an E. coli lawn (Groups A1–A10) and on 10 plates with no lawn (Groups B1–B10). Groups A and B show Tukey boxplots of the speed of locomotion of all worms on plates with an E. coli lawn (Group A) and of all worms on plates with no lawn (Group B). Nested ANOVA showed a statistically significant difference between worms on plates with an E. coli lawn and worms on plates with no lawn (p = 0.049) as well as a statistically significant effect among plates (p = 0.001; see also Table 2).
Mentions: In all experiments carried out, worms showed substantial interindividual variation in all investigated parameters. For example, speed of locomotion of N2 worms on plates with an E. coli lawn varied between 5.5 and 221 μm/s, and on plates with no lawn between 4.0 and 255 μm/s (Figure 3). Besides this, mean values of all investigated parameters showed considerable inter-plate variability. For example, mean speed of locomotion of N2 worms on plates with an E. coli lawn varied between 106 and 155 μm/s among plates, and on plates with no lawn between 112 and 186 μm/s among plates (Figure 3).

Bottom Line: In all seven investigated parameters, statistically significant differences were found in worm behavior between those moving on NGM-agar plates with an E. coli lawn and NGM-agar plates with no lawn.Furthermore, multiple test groups showed differences in interaction between variables as the parameters that significantly correlated statistically with speed of locomotion varied.The need to account for a nested design while performing statistical analyses in similar studies is also demonstrated.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroanatomy, Ludwig-Maximilians University of Munich Munich, Germany.

ABSTRACT
As a widely used and studied model organism, Caenorhabditis elegans worms offer the ability to investigate implications of behavioral change. Although, investigation of C. elegans behavioral traits has been shown, analysis is often narrowed down to measurements based off a single point, and thus cannot pick up on subtle behavioral and morphological changes. In the present study videos were captured of four different C. elegans strains grown in liquid cultures and transferred to NGM-agar plates with an E. coli lawn or with no lawn. Using an advanced software, WormLab, the full skeleton and outline of worms were tracked to determine whether the presence of food affects behavioral traits. In all seven investigated parameters, statistically significant differences were found in worm behavior between those moving on NGM-agar plates with an E. coli lawn and NGM-agar plates with no lawn. Furthermore, multiple test groups showed differences in interaction between variables as the parameters that significantly correlated statistically with speed of locomotion varied. In the present study, we demonstrate the validity of a model to analyze C. elegans behavior beyond simple speed of locomotion. The need to account for a nested design while performing statistical analyses in similar studies is also demonstrated. With extended analyses, C. elegans behavioral change can be investigated with greater sensitivity, which could have wide utility in fields such as, but not limited to, toxicology, drug discovery, and RNAi screening.

No MeSH data available.


Related in: MedlinePlus