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Comparative Proteomic Analysis Reveals Differential Root Proteins in Medicago sativa and Medicago truncatula in Response to Salt Stress.

Long R, Li M, Zhang T, Kang J, Sun Y, Cong L, Gao Y, Liu F, Yang Q - Front Plant Sci (2016)

Bottom Line: We identified 93 and 30 proteins whose abundance was significantly affected by salt stress in Zhongmu-1 and Jemalong A17 roots, respectively.The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively.Our results indicate that some of the identified proteins may play key roles in salt stress tolerance.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Beijing, China.

ABSTRACT
Salt stress is an important abiotic stress that causes decreased crop yields. Root growth and plant activities are affected by salt stress through the actions of specific genes that help roots adapt to adverse environmental conditions. For a more comprehensive understanding of proteins affected by salinity, we used two-dimensional gel electrophoresis and mass spectrometry to characterize the proteome-level changes associated with salt stress response in Medicago sativa cv. Zhongmu-1 and Medicago truncatula cv. Jemalong A17 roots. Our physiological and phenotypic observations indicated that Zhongmu-1 was more salt tolerant than Jemalong A17. We identified 93 and 30 proteins whose abundance was significantly affected by salt stress in Zhongmu-1 and Jemalong A17 roots, respectively. The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively. Function analyses indicated molecule binding and catalytic activity were the two primary functional categories. These proteins have known functions in various molecular processes, including defense against oxidative stress, metabolism, photosynthesis, protein synthesis and processing, and signal transduction. The transcript levels of four identified proteins were determined by quantitative reverse transcription polymerase chain reaction. Our results indicate that some of the identified proteins may play key roles in salt stress tolerance.

No MeSH data available.


Related in: MedlinePlus

Functional categorization of identified proteins. The identified proteins in M. truncatula cv. Jemalong A17 (A) and M. sativa cv. Zhongmu-1 (B) were grouped into 12 and 16 functional categories, respectively.
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Figure 4: Functional categorization of identified proteins. The identified proteins in M. truncatula cv. Jemalong A17 (A) and M. sativa cv. Zhongmu-1 (B) were grouped into 12 and 16 functional categories, respectively.

Mentions: The identified protein sequences were blasted by BLASTP in the NCBI database. These identified proteins in Jemalong A17 were classified into 11 functional groups based on GO prediction (Figure 4A), including binding, catalytic activity, nucleotide binding, hydrolase activity, small molecule binding, protein binding, transferase activity, RNA binding, lipid binding, transporter activity, nucleic acid binding and not determined. These identified proteins in Zhongmu-1 were classified into 15 functional groups based on GO prediction (Figure 4B), including binding, catalytic activity, nucleotide binding, small molecule binding, transferase activity, hydrolase activity, protein binding, RNA binding, nucleic acid binding, translation factor activity, transporter activity, kinase activity, transferase activity, DNA binding, enzyme regulator activity and not determined. Molecule binding group and catalytic activity group were the two mainly functional groups.


Comparative Proteomic Analysis Reveals Differential Root Proteins in Medicago sativa and Medicago truncatula in Response to Salt Stress.

Long R, Li M, Zhang T, Kang J, Sun Y, Cong L, Gao Y, Liu F, Yang Q - Front Plant Sci (2016)

Functional categorization of identified proteins. The identified proteins in M. truncatula cv. Jemalong A17 (A) and M. sativa cv. Zhongmu-1 (B) were grouped into 12 and 16 functional categories, respectively.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4814493&req=5

Figure 4: Functional categorization of identified proteins. The identified proteins in M. truncatula cv. Jemalong A17 (A) and M. sativa cv. Zhongmu-1 (B) were grouped into 12 and 16 functional categories, respectively.
Mentions: The identified protein sequences were blasted by BLASTP in the NCBI database. These identified proteins in Jemalong A17 were classified into 11 functional groups based on GO prediction (Figure 4A), including binding, catalytic activity, nucleotide binding, hydrolase activity, small molecule binding, protein binding, transferase activity, RNA binding, lipid binding, transporter activity, nucleic acid binding and not determined. These identified proteins in Zhongmu-1 were classified into 15 functional groups based on GO prediction (Figure 4B), including binding, catalytic activity, nucleotide binding, small molecule binding, transferase activity, hydrolase activity, protein binding, RNA binding, nucleic acid binding, translation factor activity, transporter activity, kinase activity, transferase activity, DNA binding, enzyme regulator activity and not determined. Molecule binding group and catalytic activity group were the two mainly functional groups.

Bottom Line: We identified 93 and 30 proteins whose abundance was significantly affected by salt stress in Zhongmu-1 and Jemalong A17 roots, respectively.The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively.Our results indicate that some of the identified proteins may play key roles in salt stress tolerance.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Beijing, China.

ABSTRACT
Salt stress is an important abiotic stress that causes decreased crop yields. Root growth and plant activities are affected by salt stress through the actions of specific genes that help roots adapt to adverse environmental conditions. For a more comprehensive understanding of proteins affected by salinity, we used two-dimensional gel electrophoresis and mass spectrometry to characterize the proteome-level changes associated with salt stress response in Medicago sativa cv. Zhongmu-1 and Medicago truncatula cv. Jemalong A17 roots. Our physiological and phenotypic observations indicated that Zhongmu-1 was more salt tolerant than Jemalong A17. We identified 93 and 30 proteins whose abundance was significantly affected by salt stress in Zhongmu-1 and Jemalong A17 roots, respectively. The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively. Function analyses indicated molecule binding and catalytic activity were the two primary functional categories. These proteins have known functions in various molecular processes, including defense against oxidative stress, metabolism, photosynthesis, protein synthesis and processing, and signal transduction. The transcript levels of four identified proteins were determined by quantitative reverse transcription polymerase chain reaction. Our results indicate that some of the identified proteins may play key roles in salt stress tolerance.

No MeSH data available.


Related in: MedlinePlus