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Comparative Proteomic Analysis Reveals Differential Root Proteins in Medicago sativa and Medicago truncatula in Response to Salt Stress.

Long R, Li M, Zhang T, Kang J, Sun Y, Cong L, Gao Y, Liu F, Yang Q - Front Plant Sci (2016)

Bottom Line: We identified 93 and 30 proteins whose abundance was significantly affected by salt stress in Zhongmu-1 and Jemalong A17 roots, respectively.The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively.Our results indicate that some of the identified proteins may play key roles in salt stress tolerance.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Beijing, China.

ABSTRACT
Salt stress is an important abiotic stress that causes decreased crop yields. Root growth and plant activities are affected by salt stress through the actions of specific genes that help roots adapt to adverse environmental conditions. For a more comprehensive understanding of proteins affected by salinity, we used two-dimensional gel electrophoresis and mass spectrometry to characterize the proteome-level changes associated with salt stress response in Medicago sativa cv. Zhongmu-1 and Medicago truncatula cv. Jemalong A17 roots. Our physiological and phenotypic observations indicated that Zhongmu-1 was more salt tolerant than Jemalong A17. We identified 93 and 30 proteins whose abundance was significantly affected by salt stress in Zhongmu-1 and Jemalong A17 roots, respectively. The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively. Function analyses indicated molecule binding and catalytic activity were the two primary functional categories. These proteins have known functions in various molecular processes, including defense against oxidative stress, metabolism, photosynthesis, protein synthesis and processing, and signal transduction. The transcript levels of four identified proteins were determined by quantitative reverse transcription polymerase chain reaction. Our results indicate that some of the identified proteins may play key roles in salt stress tolerance.

No MeSH data available.


Related in: MedlinePlus

Enlarged 2-DE gel regions of 16 differentially accumulated root proteins in M. sativa cv. Zhongmu-1 (Ms) and M. truncatula cv. Jemalong A17 (Mt). The abundance of S1, S4, S26, S35, T1, T3, T4, and T21 increased after 8 h salt stress. The abundance of S56, S59, S85, S90, T25, T26, T27, and T29 decreased after 8 h salt stress.
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Figure 3: Enlarged 2-DE gel regions of 16 differentially accumulated root proteins in M. sativa cv. Zhongmu-1 (Ms) and M. truncatula cv. Jemalong A17 (Mt). The abundance of S1, S4, S26, S35, T1, T3, T4, and T21 increased after 8 h salt stress. The abundance of S56, S59, S85, S90, T25, T26, T27, and T29 decreased after 8 h salt stress.

Mentions: The root is the first plant organ to be affected by salt stress. Representative 2-DE gel images of the Zhongmu-1 and Jemalong A17 root proteomes following salt treatment are presented in Figure 2. There was a broad distribution of the proteins in terms of pI (4.0–7.0) and mass (10–70 kDa). Of the approximately 800 detected Zhongmu-1 protein spots, 93 exhibited significant changes to spot abundance (P < 0.05) (Figure 2A, Supplementary Figure 1). Fifty-three of these proteins were up-regulated by salt stress, with the remaining 40 being down-regulated (Supplementary Table 2, Figure 3). Of the approximately 900 detected Jemalong A17 protein spots, 30 protein spots exhibited significant changes to spot abundance (P < 0.05) (Figure 2B, Supplementary Figure 1). Twenty-two of these proteins were up-regulated by salt stress, with the remaining eight being down-regulated (Supplementary Table 2, Figure 3).


Comparative Proteomic Analysis Reveals Differential Root Proteins in Medicago sativa and Medicago truncatula in Response to Salt Stress.

Long R, Li M, Zhang T, Kang J, Sun Y, Cong L, Gao Y, Liu F, Yang Q - Front Plant Sci (2016)

Enlarged 2-DE gel regions of 16 differentially accumulated root proteins in M. sativa cv. Zhongmu-1 (Ms) and M. truncatula cv. Jemalong A17 (Mt). The abundance of S1, S4, S26, S35, T1, T3, T4, and T21 increased after 8 h salt stress. The abundance of S56, S59, S85, S90, T25, T26, T27, and T29 decreased after 8 h salt stress.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4814493&req=5

Figure 3: Enlarged 2-DE gel regions of 16 differentially accumulated root proteins in M. sativa cv. Zhongmu-1 (Ms) and M. truncatula cv. Jemalong A17 (Mt). The abundance of S1, S4, S26, S35, T1, T3, T4, and T21 increased after 8 h salt stress. The abundance of S56, S59, S85, S90, T25, T26, T27, and T29 decreased after 8 h salt stress.
Mentions: The root is the first plant organ to be affected by salt stress. Representative 2-DE gel images of the Zhongmu-1 and Jemalong A17 root proteomes following salt treatment are presented in Figure 2. There was a broad distribution of the proteins in terms of pI (4.0–7.0) and mass (10–70 kDa). Of the approximately 800 detected Zhongmu-1 protein spots, 93 exhibited significant changes to spot abundance (P < 0.05) (Figure 2A, Supplementary Figure 1). Fifty-three of these proteins were up-regulated by salt stress, with the remaining 40 being down-regulated (Supplementary Table 2, Figure 3). Of the approximately 900 detected Jemalong A17 protein spots, 30 protein spots exhibited significant changes to spot abundance (P < 0.05) (Figure 2B, Supplementary Figure 1). Twenty-two of these proteins were up-regulated by salt stress, with the remaining eight being down-regulated (Supplementary Table 2, Figure 3).

Bottom Line: We identified 93 and 30 proteins whose abundance was significantly affected by salt stress in Zhongmu-1 and Jemalong A17 roots, respectively.The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively.Our results indicate that some of the identified proteins may play key roles in salt stress tolerance.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Beijing, China.

ABSTRACT
Salt stress is an important abiotic stress that causes decreased crop yields. Root growth and plant activities are affected by salt stress through the actions of specific genes that help roots adapt to adverse environmental conditions. For a more comprehensive understanding of proteins affected by salinity, we used two-dimensional gel electrophoresis and mass spectrometry to characterize the proteome-level changes associated with salt stress response in Medicago sativa cv. Zhongmu-1 and Medicago truncatula cv. Jemalong A17 roots. Our physiological and phenotypic observations indicated that Zhongmu-1 was more salt tolerant than Jemalong A17. We identified 93 and 30 proteins whose abundance was significantly affected by salt stress in Zhongmu-1 and Jemalong A17 roots, respectively. The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively. Function analyses indicated molecule binding and catalytic activity were the two primary functional categories. These proteins have known functions in various molecular processes, including defense against oxidative stress, metabolism, photosynthesis, protein synthesis and processing, and signal transduction. The transcript levels of four identified proteins were determined by quantitative reverse transcription polymerase chain reaction. Our results indicate that some of the identified proteins may play key roles in salt stress tolerance.

No MeSH data available.


Related in: MedlinePlus