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Proteomics As a Tool for Studying Bacterial Virulence and Antimicrobial Resistance.

Pérez-Llarena FJ, Bou G - Front Microbiol (2016)

Bottom Line: Proteomic studies have improved our understanding of the microbial world.This review article addresses these issues in some of the most important human pathogens.It also reports some applications of Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight (MALDI-TOF) mass spectrometry that may be important for the diagnosis of bacterial resistance in clinical laboratories in the future.

View Article: PubMed Central - PubMed

Affiliation: Servicio de Microbiología-INIBIC, Complejo Hospitalario Universitario A Coruña A Coruña, Spain.

ABSTRACT
Proteomic studies have improved our understanding of the microbial world. The most recent advances in this field have helped us to explore aspects beyond genomics. For example, by studying proteins and their regulation, researchers now understand how some pathogenic bacteria have adapted to the lethal actions of antibiotics. Proteomics has also advanced our knowledge of mechanisms of bacterial virulence and some important aspects of how bacteria interact with human cells and, thus, of the pathogenesis of infectious diseases. This review article addresses these issues in some of the most important human pathogens. It also reports some applications of Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight (MALDI-TOF) mass spectrometry that may be important for the diagnosis of bacterial resistance in clinical laboratories in the future. The reported advances will enable new diagnostic and therapeutic strategies to be developed in the fight against some of the most lethal bacteria affecting humans.

No MeSH data available.


Related in: MedlinePlus

Simplified procedure of minisequencing linked to MALDI-TOF MS detection. Obtained from Hrabák et al. (2013) and reprinted with permission from the publisher.
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Figure 3: Simplified procedure of minisequencing linked to MALDI-TOF MS detection. Obtained from Hrabák et al. (2013) and reprinted with permission from the publisher.

Mentions: MALDI-TOF MS methods have been used to analyse DNA sequencing (Pusch et al., 2002). The technique is based on a primer extension assay for only a few bases, and the molecular weight shift is then detected by MALDI-TOF MS. The technique is limited to DNA molecules smaller than 40 bp (Figure 3).


Proteomics As a Tool for Studying Bacterial Virulence and Antimicrobial Resistance.

Pérez-Llarena FJ, Bou G - Front Microbiol (2016)

Simplified procedure of minisequencing linked to MALDI-TOF MS detection. Obtained from Hrabák et al. (2013) and reprinted with permission from the publisher.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814472&req=5

Figure 3: Simplified procedure of minisequencing linked to MALDI-TOF MS detection. Obtained from Hrabák et al. (2013) and reprinted with permission from the publisher.
Mentions: MALDI-TOF MS methods have been used to analyse DNA sequencing (Pusch et al., 2002). The technique is based on a primer extension assay for only a few bases, and the molecular weight shift is then detected by MALDI-TOF MS. The technique is limited to DNA molecules smaller than 40 bp (Figure 3).

Bottom Line: Proteomic studies have improved our understanding of the microbial world.This review article addresses these issues in some of the most important human pathogens.It also reports some applications of Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight (MALDI-TOF) mass spectrometry that may be important for the diagnosis of bacterial resistance in clinical laboratories in the future.

View Article: PubMed Central - PubMed

Affiliation: Servicio de Microbiología-INIBIC, Complejo Hospitalario Universitario A Coruña A Coruña, Spain.

ABSTRACT
Proteomic studies have improved our understanding of the microbial world. The most recent advances in this field have helped us to explore aspects beyond genomics. For example, by studying proteins and their regulation, researchers now understand how some pathogenic bacteria have adapted to the lethal actions of antibiotics. Proteomics has also advanced our knowledge of mechanisms of bacterial virulence and some important aspects of how bacteria interact with human cells and, thus, of the pathogenesis of infectious diseases. This review article addresses these issues in some of the most important human pathogens. It also reports some applications of Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight (MALDI-TOF) mass spectrometry that may be important for the diagnosis of bacterial resistance in clinical laboratories in the future. The reported advances will enable new diagnostic and therapeutic strategies to be developed in the fight against some of the most lethal bacteria affecting humans.

No MeSH data available.


Related in: MedlinePlus