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Insight to Nanoparticle Size Analysis-Novel and Convenient Image Analysis Method Versus Conventional Techniques.

Vippola M, Valkonen M, Sarlin E, Honkanen M, Huttunen H - Nanoscale Res Lett (2016)

Bottom Line: However, particle shapes that are very different from spherical proved to be problematic also for the novel program.When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles.However, the SAXS method does not provide any data about the shape or appearance of the sample.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science, Tampere University of Technology, P.O. Box 589, 33101, Tampere, Finland. minnamari.vippola@tut.fi.

ABSTRACT
The aim of this paper is to introduce a new image analysis program "Nanoannotator" particularly developed for analyzing individual nanoparticles in transmission electron microscopy images. This paper describes the usefulness and efficiency of the program when analyzing nanoparticles, and at the same time, we compare it to more conventional nanoparticle analysis techniques. The techniques which we are concentrating here are transmission electron microscopy (TEM) linked with different image analysis methods and X-ray diffraction techniques. The developed program appeared as a good supplement to the field of particle analysis techniques, since the traditional image analysis programs suffer from the inability to separate the individual particles from agglomerates in the TEM images. The program is more efficient, and it offers more detailed morphological information of the particles than the manual technique. However, particle shapes that are very different from spherical proved to be problematic also for the novel program. When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles. However, the SAXS method does not provide any data about the shape or appearance of the sample.

No MeSH data available.


Related in: MedlinePlus

The size distribution of iron whiskers (a) by number as a result of image analysis method and (b) by volume as a result of SAXS analysis
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Fig7: The size distribution of iron whiskers (a) by number as a result of image analysis method and (b) by volume as a result of SAXS analysis

Mentions: The possible problem of the SAXS method lies in analyzing the obtained data. In general, SAXS analysis methods presume a constant shape for the nanoparticles [8], and in SAXS software, it is typically spherical. This is also the case for the EasySAXS software (version 2.0a) where the calculation of the volume distributions is available only for spherical nanoparticles. Thus, the default assumption of spherical shape of particles is false and causes error in the results. The differences in the SAXS and image analysis results are illustrated in Fig. 7 in which the Nanoannotator results (average equivalent diameter) and SAXS results (diameter) are compared. The binary distribution of the image analysis technique results reflects the needle-like shape of the particles (whisker length and width) whereas the presumption of spherical particles loses the effect from the SAXS results. For shapes which are different from spheres, one can do simulations and fitting in SAXS software, but it will provide only average values of the dimensions.Fig. 7


Insight to Nanoparticle Size Analysis-Novel and Convenient Image Analysis Method Versus Conventional Techniques.

Vippola M, Valkonen M, Sarlin E, Honkanen M, Huttunen H - Nanoscale Res Lett (2016)

The size distribution of iron whiskers (a) by number as a result of image analysis method and (b) by volume as a result of SAXS analysis
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814392&req=5

Fig7: The size distribution of iron whiskers (a) by number as a result of image analysis method and (b) by volume as a result of SAXS analysis
Mentions: The possible problem of the SAXS method lies in analyzing the obtained data. In general, SAXS analysis methods presume a constant shape for the nanoparticles [8], and in SAXS software, it is typically spherical. This is also the case for the EasySAXS software (version 2.0a) where the calculation of the volume distributions is available only for spherical nanoparticles. Thus, the default assumption of spherical shape of particles is false and causes error in the results. The differences in the SAXS and image analysis results are illustrated in Fig. 7 in which the Nanoannotator results (average equivalent diameter) and SAXS results (diameter) are compared. The binary distribution of the image analysis technique results reflects the needle-like shape of the particles (whisker length and width) whereas the presumption of spherical particles loses the effect from the SAXS results. For shapes which are different from spheres, one can do simulations and fitting in SAXS software, but it will provide only average values of the dimensions.Fig. 7

Bottom Line: However, particle shapes that are very different from spherical proved to be problematic also for the novel program.When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles.However, the SAXS method does not provide any data about the shape or appearance of the sample.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science, Tampere University of Technology, P.O. Box 589, 33101, Tampere, Finland. minnamari.vippola@tut.fi.

ABSTRACT
The aim of this paper is to introduce a new image analysis program "Nanoannotator" particularly developed for analyzing individual nanoparticles in transmission electron microscopy images. This paper describes the usefulness and efficiency of the program when analyzing nanoparticles, and at the same time, we compare it to more conventional nanoparticle analysis techniques. The techniques which we are concentrating here are transmission electron microscopy (TEM) linked with different image analysis methods and X-ray diffraction techniques. The developed program appeared as a good supplement to the field of particle analysis techniques, since the traditional image analysis programs suffer from the inability to separate the individual particles from agglomerates in the TEM images. The program is more efficient, and it offers more detailed morphological information of the particles than the manual technique. However, particle shapes that are very different from spherical proved to be problematic also for the novel program. When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles. However, the SAXS method does not provide any data about the shape or appearance of the sample.

No MeSH data available.


Related in: MedlinePlus