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Insight to Nanoparticle Size Analysis-Novel and Convenient Image Analysis Method Versus Conventional Techniques.

Vippola M, Valkonen M, Sarlin E, Honkanen M, Huttunen H - Nanoscale Res Lett (2016)

Bottom Line: However, particle shapes that are very different from spherical proved to be problematic also for the novel program.When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles.However, the SAXS method does not provide any data about the shape or appearance of the sample.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science, Tampere University of Technology, P.O. Box 589, 33101, Tampere, Finland. minnamari.vippola@tut.fi.

ABSTRACT
The aim of this paper is to introduce a new image analysis program "Nanoannotator" particularly developed for analyzing individual nanoparticles in transmission electron microscopy images. This paper describes the usefulness and efficiency of the program when analyzing nanoparticles, and at the same time, we compare it to more conventional nanoparticle analysis techniques. The techniques which we are concentrating here are transmission electron microscopy (TEM) linked with different image analysis methods and X-ray diffraction techniques. The developed program appeared as a good supplement to the field of particle analysis techniques, since the traditional image analysis programs suffer from the inability to separate the individual particles from agglomerates in the TEM images. The program is more efficient, and it offers more detailed morphological information of the particles than the manual technique. However, particle shapes that are very different from spherical proved to be problematic also for the novel program. When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles. However, the SAXS method does not provide any data about the shape or appearance of the sample.

No MeSH data available.


Related in: MedlinePlus

Graphite nanoparticles: individual particles are difficult to identify from the agglomerates and the contrast formation due to the inner structure of the particles complicates the analysis further
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Fig6: Graphite nanoparticles: individual particles are difficult to identify from the agglomerates and the contrast formation due to the inner structure of the particles complicates the analysis further

Mentions: The small and roundish graphite nanoparticles were also problematic to analyze with the Nanoannotator program: the tendency of the particles to agglomerate and the ability of TEM to resolve the inner structure of the graphite particles (Fig. 6) make image analysis and evaluation of its success almost impossible. Thus, the graphite particles were not analyzed at all with image analysis techniques.Fig. 6


Insight to Nanoparticle Size Analysis-Novel and Convenient Image Analysis Method Versus Conventional Techniques.

Vippola M, Valkonen M, Sarlin E, Honkanen M, Huttunen H - Nanoscale Res Lett (2016)

Graphite nanoparticles: individual particles are difficult to identify from the agglomerates and the contrast formation due to the inner structure of the particles complicates the analysis further
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814392&req=5

Fig6: Graphite nanoparticles: individual particles are difficult to identify from the agglomerates and the contrast formation due to the inner structure of the particles complicates the analysis further
Mentions: The small and roundish graphite nanoparticles were also problematic to analyze with the Nanoannotator program: the tendency of the particles to agglomerate and the ability of TEM to resolve the inner structure of the graphite particles (Fig. 6) make image analysis and evaluation of its success almost impossible. Thus, the graphite particles were not analyzed at all with image analysis techniques.Fig. 6

Bottom Line: However, particle shapes that are very different from spherical proved to be problematic also for the novel program.When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles.However, the SAXS method does not provide any data about the shape or appearance of the sample.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science, Tampere University of Technology, P.O. Box 589, 33101, Tampere, Finland. minnamari.vippola@tut.fi.

ABSTRACT
The aim of this paper is to introduce a new image analysis program "Nanoannotator" particularly developed for analyzing individual nanoparticles in transmission electron microscopy images. This paper describes the usefulness and efficiency of the program when analyzing nanoparticles, and at the same time, we compare it to more conventional nanoparticle analysis techniques. The techniques which we are concentrating here are transmission electron microscopy (TEM) linked with different image analysis methods and X-ray diffraction techniques. The developed program appeared as a good supplement to the field of particle analysis techniques, since the traditional image analysis programs suffer from the inability to separate the individual particles from agglomerates in the TEM images. The program is more efficient, and it offers more detailed morphological information of the particles than the manual technique. However, particle shapes that are very different from spherical proved to be problematic also for the novel program. When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles. However, the SAXS method does not provide any data about the shape or appearance of the sample.

No MeSH data available.


Related in: MedlinePlus