Limits...
Insight to Nanoparticle Size Analysis-Novel and Convenient Image Analysis Method Versus Conventional Techniques.

Vippola M, Valkonen M, Sarlin E, Honkanen M, Huttunen H - Nanoscale Res Lett (2016)

Bottom Line: However, particle shapes that are very different from spherical proved to be problematic also for the novel program.When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles.However, the SAXS method does not provide any data about the shape or appearance of the sample.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science, Tampere University of Technology, P.O. Box 589, 33101, Tampere, Finland. minnamari.vippola@tut.fi.

ABSTRACT
The aim of this paper is to introduce a new image analysis program "Nanoannotator" particularly developed for analyzing individual nanoparticles in transmission electron microscopy images. This paper describes the usefulness and efficiency of the program when analyzing nanoparticles, and at the same time, we compare it to more conventional nanoparticle analysis techniques. The techniques which we are concentrating here are transmission electron microscopy (TEM) linked with different image analysis methods and X-ray diffraction techniques. The developed program appeared as a good supplement to the field of particle analysis techniques, since the traditional image analysis programs suffer from the inability to separate the individual particles from agglomerates in the TEM images. The program is more efficient, and it offers more detailed morphological information of the particles than the manual technique. However, particle shapes that are very different from spherical proved to be problematic also for the novel program. When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles. However, the SAXS method does not provide any data about the shape or appearance of the sample.

No MeSH data available.


Related in: MedlinePlus

Silver nanoparticles (a), iron oxide whiskers (b), and graphite nanoparticles (c) used in the study. Note the different scale bars in the images
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4814392&req=5

Fig1: Silver nanoparticles (a), iron oxide whiskers (b), and graphite nanoparticles (c) used in the study. Note the different scale bars in the images

Mentions: Examples of the studied materials are shown in Fig. 1. The silver and the graphite nanoparticles are quite circular whereas the iron oxide whiskers are needle-like particles. The silver nanoparticles are larger, and the individual particles are much easier to distinguish by the naked eye from a TEM image when compared to the graphite. Instead, the graphite nanoparticles tend to agglomerate, and their small size and poorer contrast due to lighter weight make it difficult to perceive the contours of the particles from the TEM images even by the naked eye. Also, the individual iron oxide whiskers were occasionally difficult to separate from a TEM image due to their variable size and surface topography. In the following sections, the methods, the particle size distribution results and the practicality of the different image analysis methods, and the SAXS technique are described.Fig. 1


Insight to Nanoparticle Size Analysis-Novel and Convenient Image Analysis Method Versus Conventional Techniques.

Vippola M, Valkonen M, Sarlin E, Honkanen M, Huttunen H - Nanoscale Res Lett (2016)

Silver nanoparticles (a), iron oxide whiskers (b), and graphite nanoparticles (c) used in the study. Note the different scale bars in the images
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814392&req=5

Fig1: Silver nanoparticles (a), iron oxide whiskers (b), and graphite nanoparticles (c) used in the study. Note the different scale bars in the images
Mentions: Examples of the studied materials are shown in Fig. 1. The silver and the graphite nanoparticles are quite circular whereas the iron oxide whiskers are needle-like particles. The silver nanoparticles are larger, and the individual particles are much easier to distinguish by the naked eye from a TEM image when compared to the graphite. Instead, the graphite nanoparticles tend to agglomerate, and their small size and poorer contrast due to lighter weight make it difficult to perceive the contours of the particles from the TEM images even by the naked eye. Also, the individual iron oxide whiskers were occasionally difficult to separate from a TEM image due to their variable size and surface topography. In the following sections, the methods, the particle size distribution results and the practicality of the different image analysis methods, and the SAXS technique are described.Fig. 1

Bottom Line: However, particle shapes that are very different from spherical proved to be problematic also for the novel program.When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles.However, the SAXS method does not provide any data about the shape or appearance of the sample.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science, Tampere University of Technology, P.O. Box 589, 33101, Tampere, Finland. minnamari.vippola@tut.fi.

ABSTRACT
The aim of this paper is to introduce a new image analysis program "Nanoannotator" particularly developed for analyzing individual nanoparticles in transmission electron microscopy images. This paper describes the usefulness and efficiency of the program when analyzing nanoparticles, and at the same time, we compare it to more conventional nanoparticle analysis techniques. The techniques which we are concentrating here are transmission electron microscopy (TEM) linked with different image analysis methods and X-ray diffraction techniques. The developed program appeared as a good supplement to the field of particle analysis techniques, since the traditional image analysis programs suffer from the inability to separate the individual particles from agglomerates in the TEM images. The program is more efficient, and it offers more detailed morphological information of the particles than the manual technique. However, particle shapes that are very different from spherical proved to be problematic also for the novel program. When compared to X-ray techniques, the main advantage of the small-angle X-ray scattering (SAXS) method is the average data it provides from a very large amount of particles. However, the SAXS method does not provide any data about the shape or appearance of the sample.

No MeSH data available.


Related in: MedlinePlus