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TiLIA: a software package for image analysis of firefly flash patterns.

Konno J, Hatta-Ohashi Y, Akiyoshi R, Thancharoen A, Silalom S, Sakchoowong W, Yiu V, Ohba N, Suzuki H - Ecol Evol (2016)

Bottom Line: As flash signaling patterns of fireflies are species specific, signal-pattern analysis is important for understanding this system of communication.TiLIA enables flight path tracing of individual fireflies and provides frame-by-frame coordinates and light intensity data.As an example of TiLIA capabilities, we demonstrate flash pattern analysis of the fireflies Luciola cruciata and L. lateralis during courtship behavior.

View Article: PubMed Central - PubMed

Affiliation: Advanced Analysis Technology Department Olympus Corporation Kuboyama 2-3 Hachioji Tokyo 192-8512 Japan.

ABSTRACT
As flash signaling patterns of fireflies are species specific, signal-pattern analysis is important for understanding this system of communication. Here, we present time-lapse image analysis (TiLIA), a free open-source software package for signal and flight pattern analyses of fireflies that uses video-recorded image data. TiLIA enables flight path tracing of individual fireflies and provides frame-by-frame coordinates and light intensity data. As an example of TiLIA capabilities, we demonstrate flash pattern analysis of the fireflies Luciola cruciata and L. lateralis during courtship behavior.

No MeSH data available.


Related in: MedlinePlus

The graphical user interface of TiLIA, which is composed of several spaces and areas: (A) image display space, (B) data analysis space, (C) image property adjustment area, (D) ROI select area, (E) video operation area, and (F) command area. The five ROIs are assigned to Luciola cruciata fireflies individually in the image display space (A), and the fireflies are then traced within the ROI manually frame by frame. The time course of light intensity for each ROI is simultaneously displayed as a graph in the data analysis space (B). The flash patterns are described as four flashes of single peak within 8.0 sec except for the blue one (three peaks), and the flash duration and peak interval are 1.3 and 1.9 sec, respectively. The flashes are loosely synchronous. The red vertical line indicates the frame number of the image displayed.
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ece32078-fig-0002: The graphical user interface of TiLIA, which is composed of several spaces and areas: (A) image display space, (B) data analysis space, (C) image property adjustment area, (D) ROI select area, (E) video operation area, and (F) command area. The five ROIs are assigned to Luciola cruciata fireflies individually in the image display space (A), and the fireflies are then traced within the ROI manually frame by frame. The time course of light intensity for each ROI is simultaneously displayed as a graph in the data analysis space (B). The flash patterns are described as four flashes of single peak within 8.0 sec except for the blue one (three peaks), and the flash duration and peak interval are 1.3 and 1.9 sec, respectively. The flashes are loosely synchronous. The red vertical line indicates the frame number of the image displayed.

Mentions: TiLIA is operated with the following three steps (Fig. 1): A) trim a scene of video for analysis and convert it to audio video interleave (AVI)‐format file; B) trace the flight path of object and analyze the flight path and light intensity; and C) record the output results as image, video, and text data. Figure 2 shows the graphical user interface of TiLIA, which is composed of several spaces and areas: A) image display space, B) data analysis space, C) image property adjustment area, D) region of interest (ROI) select area, E) video operation area, and F) command area. An AVI‐format video file of a luminescent object is loaded into TiLIA, and each frame is saved as a separate tagged image file format (TIFF) file. The brightness and contrast of each image are adjusted (original image file is not rewritten), and the appropriate size of the ROI is assigned to an object in the image display space (Fig. 2A). The object is then manually (by mouse) traced frame by frame. This process is very time‐consuming; therefore, a scene selected for analysis is trimmed appropriately using a video editing software or TiLIA in advance. The light intensity value of the ROI in each frame is recorded, and the resulting time course is plotted graphically in the data analysis space (Fig. 2B). The tracing process can be confirmed by viewing the reintegrated video file, and the position of the ROI can be adjusted as necessary. The flight track of the ROI can also be displayed in the video. The graph of time course of light intensity (Fig. 2B) is saved as an image file, and the light intensity values are stored as text data. If flight paths of two fireflies coincide, the two assigned ROIs trace the two paths superpositionally. However, it is impossible to distinguish between the light intensities of two fireflies. Images of the ROI and of flight path, and the ROI coordinates data are also stored.


TiLIA: a software package for image analysis of firefly flash patterns.

Konno J, Hatta-Ohashi Y, Akiyoshi R, Thancharoen A, Silalom S, Sakchoowong W, Yiu V, Ohba N, Suzuki H - Ecol Evol (2016)

The graphical user interface of TiLIA, which is composed of several spaces and areas: (A) image display space, (B) data analysis space, (C) image property adjustment area, (D) ROI select area, (E) video operation area, and (F) command area. The five ROIs are assigned to Luciola cruciata fireflies individually in the image display space (A), and the fireflies are then traced within the ROI manually frame by frame. The time course of light intensity for each ROI is simultaneously displayed as a graph in the data analysis space (B). The flash patterns are described as four flashes of single peak within 8.0 sec except for the blue one (three peaks), and the flash duration and peak interval are 1.3 and 1.9 sec, respectively. The flashes are loosely synchronous. The red vertical line indicates the frame number of the image displayed.
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ece32078-fig-0002: The graphical user interface of TiLIA, which is composed of several spaces and areas: (A) image display space, (B) data analysis space, (C) image property adjustment area, (D) ROI select area, (E) video operation area, and (F) command area. The five ROIs are assigned to Luciola cruciata fireflies individually in the image display space (A), and the fireflies are then traced within the ROI manually frame by frame. The time course of light intensity for each ROI is simultaneously displayed as a graph in the data analysis space (B). The flash patterns are described as four flashes of single peak within 8.0 sec except for the blue one (three peaks), and the flash duration and peak interval are 1.3 and 1.9 sec, respectively. The flashes are loosely synchronous. The red vertical line indicates the frame number of the image displayed.
Mentions: TiLIA is operated with the following three steps (Fig. 1): A) trim a scene of video for analysis and convert it to audio video interleave (AVI)‐format file; B) trace the flight path of object and analyze the flight path and light intensity; and C) record the output results as image, video, and text data. Figure 2 shows the graphical user interface of TiLIA, which is composed of several spaces and areas: A) image display space, B) data analysis space, C) image property adjustment area, D) region of interest (ROI) select area, E) video operation area, and F) command area. An AVI‐format video file of a luminescent object is loaded into TiLIA, and each frame is saved as a separate tagged image file format (TIFF) file. The brightness and contrast of each image are adjusted (original image file is not rewritten), and the appropriate size of the ROI is assigned to an object in the image display space (Fig. 2A). The object is then manually (by mouse) traced frame by frame. This process is very time‐consuming; therefore, a scene selected for analysis is trimmed appropriately using a video editing software or TiLIA in advance. The light intensity value of the ROI in each frame is recorded, and the resulting time course is plotted graphically in the data analysis space (Fig. 2B). The tracing process can be confirmed by viewing the reintegrated video file, and the position of the ROI can be adjusted as necessary. The flight track of the ROI can also be displayed in the video. The graph of time course of light intensity (Fig. 2B) is saved as an image file, and the light intensity values are stored as text data. If flight paths of two fireflies coincide, the two assigned ROIs trace the two paths superpositionally. However, it is impossible to distinguish between the light intensities of two fireflies. Images of the ROI and of flight path, and the ROI coordinates data are also stored.

Bottom Line: As flash signaling patterns of fireflies are species specific, signal-pattern analysis is important for understanding this system of communication.TiLIA enables flight path tracing of individual fireflies and provides frame-by-frame coordinates and light intensity data.As an example of TiLIA capabilities, we demonstrate flash pattern analysis of the fireflies Luciola cruciata and L. lateralis during courtship behavior.

View Article: PubMed Central - PubMed

Affiliation: Advanced Analysis Technology Department Olympus Corporation Kuboyama 2-3 Hachioji Tokyo 192-8512 Japan.

ABSTRACT
As flash signaling patterns of fireflies are species specific, signal-pattern analysis is important for understanding this system of communication. Here, we present time-lapse image analysis (TiLIA), a free open-source software package for signal and flight pattern analyses of fireflies that uses video-recorded image data. TiLIA enables flight path tracing of individual fireflies and provides frame-by-frame coordinates and light intensity data. As an example of TiLIA capabilities, we demonstrate flash pattern analysis of the fireflies Luciola cruciata and L. lateralis during courtship behavior.

No MeSH data available.


Related in: MedlinePlus