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Corneal endothelial cells possess an elaborate multipolar shape to maximize the basolateral to apical membrane area.

Harrison TA, He Z, Boggs K, Thuret G, Liu HX, Defoe DM - Mol. Vis. (2016)

Bottom Line: The resulting overlap allows individual cells to extend over a greater area than if the cell boundaries were mutually exclusive.Our analysis indicates that, far from being simple polygonal prisms, endothelial cells possess an elaborate multipolar shape.Their unusual geometry may be essential for the endothelium to carry out its role as the principal regulator of corneal extracellular fluid flux, and thus ultimately of tissue clarity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, Quillen College of Medicine, East Tennessee State University, Johnson City, TN.

ABSTRACT

Purpose: The corneal endothelium is widely believed to consist of geometrically regular cells interconnected by junctional complexes. However, while en face visualization of the endothelial apical surface reveals characteristic polygonal borders, the overall form of the component cells has rarely been observed.

Methods: To visualize the shape of individual endothelial cells within the native monolayer, two independent Cre/LoxP-based cell labeling approaches were used. In the first, a P0-Cre mouse driver strain was bred to an R26-tdTomato reporter line to map neural crest-derived endothelial cells with cytosolic red fluorescent protein. In the second, HPRT-Cre induction of small numbers of green and red fluorescent protein-filled cells within a background of unlabeled cells was achieved using a dual-color reporter system, mosaic analysis with double markers (MADM). Selective imaging of the endothelial lateral membranes at different apicobasal levels was accomplished after staining with antibodies to ZO-1 and the neural cell adhesion molecule (NCAM).

Results: When viewed in their entirety in whole-mount preparations, fluorescent protein-filled cells appear star-shaped, extending multiple dendritic processes that radiate outward in the plane of the monolayer. Examination of rare cases where cells expressing different fluorescent proteins lie directly adjacent to one another reveals that these long processes undergo extensive interdigitation. The resulting overlap allows individual cells to extend over a greater area than if the cell boundaries were mutually exclusive. Anti-NCAM staining of these interlocking peripheral cell extensions reveals an elaborate system of lateral membrane folds that, when viewed in optical sections, increase in complexity from the apical to the basal pole. This not only produces a substantial increase in the basolateral, relative to the apical, membrane but also greatly extends the paracellular pathway as a highly convoluted space.

Conclusions: Our analysis indicates that, far from being simple polygonal prisms, endothelial cells possess an elaborate multipolar shape. Their unusual geometry may be essential for the endothelium to carry out its role as the principal regulator of corneal extracellular fluid flux, and thus ultimately of tissue clarity.

No MeSH data available.


Related in: MedlinePlus

Immunolocalization of corneal endothelial cell lateral membrane markers by widefield fluorescence microscopy. A: Reaction of intact tissues with ZO-1 antibody highlights the regular polygonal outlines of cells seen at the level of tight junctions. B: Staining for the cell adhesion protein neural cell adhesion molecule (NCAM), however, reveals that much of the interacting surface is in the form of a complex arrangement of membrane folds. When viewing whole cells, this gives the impression of extensive membrane ruffling.
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f3: Immunolocalization of corneal endothelial cell lateral membrane markers by widefield fluorescence microscopy. A: Reaction of intact tissues with ZO-1 antibody highlights the regular polygonal outlines of cells seen at the level of tight junctions. B: Staining for the cell adhesion protein neural cell adhesion molecule (NCAM), however, reveals that much of the interacting surface is in the form of a complex arrangement of membrane folds. When viewing whole cells, this gives the impression of extensive membrane ruffling.

Mentions: In addition to viewing the morphology of whole fluorophore-filled cells, we also examined CECs labeled with probes specific for lateral membrane components. When the apical poles of the cells are imaged by immunolocalization of the tight junction–associated protein ZO-1, individual cells exhibit the familiar polygonal outlines seen previously (Figure 3A). However, an antibody directed against the basolateral marker NCAM [16] reveals a highly elaborate arrangement of membrane ruffles or folds (Figure 3B), as shown recently for the human corneal endothelium [17].


Corneal endothelial cells possess an elaborate multipolar shape to maximize the basolateral to apical membrane area.

Harrison TA, He Z, Boggs K, Thuret G, Liu HX, Defoe DM - Mol. Vis. (2016)

Immunolocalization of corneal endothelial cell lateral membrane markers by widefield fluorescence microscopy. A: Reaction of intact tissues with ZO-1 antibody highlights the regular polygonal outlines of cells seen at the level of tight junctions. B: Staining for the cell adhesion protein neural cell adhesion molecule (NCAM), however, reveals that much of the interacting surface is in the form of a complex arrangement of membrane folds. When viewing whole cells, this gives the impression of extensive membrane ruffling.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814271&req=5

f3: Immunolocalization of corneal endothelial cell lateral membrane markers by widefield fluorescence microscopy. A: Reaction of intact tissues with ZO-1 antibody highlights the regular polygonal outlines of cells seen at the level of tight junctions. B: Staining for the cell adhesion protein neural cell adhesion molecule (NCAM), however, reveals that much of the interacting surface is in the form of a complex arrangement of membrane folds. When viewing whole cells, this gives the impression of extensive membrane ruffling.
Mentions: In addition to viewing the morphology of whole fluorophore-filled cells, we also examined CECs labeled with probes specific for lateral membrane components. When the apical poles of the cells are imaged by immunolocalization of the tight junction–associated protein ZO-1, individual cells exhibit the familiar polygonal outlines seen previously (Figure 3A). However, an antibody directed against the basolateral marker NCAM [16] reveals a highly elaborate arrangement of membrane ruffles or folds (Figure 3B), as shown recently for the human corneal endothelium [17].

Bottom Line: The resulting overlap allows individual cells to extend over a greater area than if the cell boundaries were mutually exclusive.Our analysis indicates that, far from being simple polygonal prisms, endothelial cells possess an elaborate multipolar shape.Their unusual geometry may be essential for the endothelium to carry out its role as the principal regulator of corneal extracellular fluid flux, and thus ultimately of tissue clarity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, Quillen College of Medicine, East Tennessee State University, Johnson City, TN.

ABSTRACT

Purpose: The corneal endothelium is widely believed to consist of geometrically regular cells interconnected by junctional complexes. However, while en face visualization of the endothelial apical surface reveals characteristic polygonal borders, the overall form of the component cells has rarely been observed.

Methods: To visualize the shape of individual endothelial cells within the native monolayer, two independent Cre/LoxP-based cell labeling approaches were used. In the first, a P0-Cre mouse driver strain was bred to an R26-tdTomato reporter line to map neural crest-derived endothelial cells with cytosolic red fluorescent protein. In the second, HPRT-Cre induction of small numbers of green and red fluorescent protein-filled cells within a background of unlabeled cells was achieved using a dual-color reporter system, mosaic analysis with double markers (MADM). Selective imaging of the endothelial lateral membranes at different apicobasal levels was accomplished after staining with antibodies to ZO-1 and the neural cell adhesion molecule (NCAM).

Results: When viewed in their entirety in whole-mount preparations, fluorescent protein-filled cells appear star-shaped, extending multiple dendritic processes that radiate outward in the plane of the monolayer. Examination of rare cases where cells expressing different fluorescent proteins lie directly adjacent to one another reveals that these long processes undergo extensive interdigitation. The resulting overlap allows individual cells to extend over a greater area than if the cell boundaries were mutually exclusive. Anti-NCAM staining of these interlocking peripheral cell extensions reveals an elaborate system of lateral membrane folds that, when viewed in optical sections, increase in complexity from the apical to the basal pole. This not only produces a substantial increase in the basolateral, relative to the apical, membrane but also greatly extends the paracellular pathway as a highly convoluted space.

Conclusions: Our analysis indicates that, far from being simple polygonal prisms, endothelial cells possess an elaborate multipolar shape. Their unusual geometry may be essential for the endothelium to carry out its role as the principal regulator of corneal extracellular fluid flux, and thus ultimately of tissue clarity.

No MeSH data available.


Related in: MedlinePlus