Sensitive cytometry based system for enumeration, capture and analysis of gene mutations of circulating tumor cells.
Bottom Line: We compared the number of CTC detected using our system with those detected using the CellSearch system in 46 patients with various cancers.The percentage of samples with detected CTC was significantly higher with our system (65.2%) than with CellSearch (28.3%).The number of detected CTC per patient using our system was statistically higher than that using CellSearch (median 5, 0; P = 0.000172, Wilcoxon test).
Affiliation: Shien-Lab, National Cancer Center Hospital, Tokyo, Japan.Show MeSH
Related in: MedlinePlus
Mentions: Circulating tumor cell enrichment from 4 mL of peripheral blood includes hemolysis and elimination of leukocytes by using CD45 antibody‐conjugated microbeads. After the enrichment process, the CTC‐enriched samples were subsequently fixed and stained using anti‐CK‐FITC, anti‐EpCAM‐PE and anti‐CD45‐Alexa Fluor 700 antibodies. Then, the samples were applied to FISHMAN‐R or On‐chip Sort. A flowchart of this process including the CTC enrichment percentage is shown in Figure 2.
Affiliation: Shien-Lab, National Cancer Center Hospital, Tokyo, Japan.