Sensitive cytometry based system for enumeration, capture and analysis of gene mutations of circulating tumor cells.
Bottom Line: We compared the number of CTC detected using our system with those detected using the CellSearch system in 46 patients with various cancers.The percentage of samples with detected CTC was significantly higher with our system (65.2%) than with CellSearch (28.3%).The number of detected CTC per patient using our system was statistically higher than that using CellSearch (median 5, 0; P = 0.000172, Wilcoxon test).
Affiliation: Shien-Lab, National Cancer Center Hospital, Tokyo, Japan.Show MeSH
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Mentions: The novel multicolor flow‐cytometry‐based CTC detection and capturing system of this article utilized FISHMAN‐R (On‐Chip Biotechnologies) and On‐Chip Sort (On‐Chip Biotechnologies), which is a cell‐sorter integrated with FISHMAN‐R. These devices use a disposable microfluidics chip and include a collection reservoir within which the sorted cells are stored (Fig. 1a–c). This design enables reduction of sample loss to the absolute minimum; specifically, the dead volume of analysis using this chip is <0.01 μL. This system therefore provides suitable conditions for the enumeration of rare cells such as CTC from patients' blood. The details of the FISHMAN‐R and On‐Chip Sort were described previously.8, 9, 10 Data analysis was performed using FlowJo software v7.6.5 (Tree Star, Ashland, OR, USA). The principle of sorting is described on the manufacturer's website (http://www.on-chip.co.jp/en/index.html).
Affiliation: Shien-Lab, National Cancer Center Hospital, Tokyo, Japan.