RAP80 regulates epithelial-mesenchymal transition related with metastasis and malignancy of cancer.
Bottom Line: The downregulation of RAP80 increases ZEB1 protein and decreases miR200c expression to activate EMT signaling in the form of drastic inhibitions of E-cadherin, p16 and p21 expression.Using in vivo metastasis analysis, RAP80 knockdown cells are shown to dramatically metastasize into the lung and generate more malignant phenotype compared to controls.Interestingly, the expression level of RAP80 was positively correlated with the survival rate in lung adenocarcinoma and breast cancer patients.
Affiliation: Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea.Show MeSH
Related in: MedlinePlus
Mentions: Metastasis and malignancy of cancer has been closely related with EMT activation. Because we show that EMT is activated by RAP80 knockdown, it is important to determine whether metastasis is also regulated by RAP80. To investigate the role of RAP80 on metastasis, control and RAP80 knockdown cells were labeled with ICG for NIR imaging and injected i.v. into Balb/C nude mice to analyze the amount of metastasis into the lung. As shown in Figure 5, RAP80 knockdown cells largely accumulated in the lung at 1, 2 and 7 days after injection, compared with control vector‐infected cell injected mice. These data also indicate that the downregulation of RAP80 activates metastasis to the lung.
Affiliation: Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea.