Novel combination of serum microRNA for detecting breast cancer in the early stage.
Bottom Line: The training cohort was used to identify a combination of miRNA that could detect breast cancer, and the test cohort was used to validate that combination. miRNA expressions were compared between patients with breast cancer and non-breast cancer, and a combination of five miRNA (miR-1246, miR-1307-3p, miR-4634, miR-6861-5p and miR-6875-5p) was found to be able to detect breast cancer.This combination had a sensitivity of 97.3%, specificity of 82.9% and accuracy of 89.7% for breast cancer in the test cohort.In addition, this combination could detect early stage breast cancer (sensitivity of 98.0% for Tis).
Affiliation: Department of Breast and Medical Oncology, National Cancer Center Hospital, Tokyo, Japan.Show MeSH
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Mentions: Using Fisher's linear discriminant analysis, we designed comprehensive discriminants with 1–5 miRNA in the training cohort (breast cancer versus non‐cancer or other cancer/non‐breast benign diseases) and validated them in the test cohort. The analysis identified a combination of five miRNA (miR‐1246, miR‐1307‐3p, miR‐4634, miR‐6861‐5p and miR‐6875‐5p) that showed the best discrimination in both the training cohort and test cohort. The expression levels of these five miRNA are presented in Figure 1. The diagnostic index was calculated using the following formula: (0.25 × miR‐1246) + (0.49 × miR‐1307‐3p) − (1.06 × miR‐4634) + (1.89 × miR‐6875‐5p) + (0.31 × miR‐6861‐5p) − 13.94. In the training cohort, the diagnostic index showed a sensitivity of 87.8%, specificity of 78.5% and accuracy of 78.7%. Although the serum samples of breast cancer patients in the test cohort were independent from those in the training cohort, the diagnostic index showed high performance (Fig. 2a,b), with a sensitivity of 97.3%, specificity of 82.9% and accuracy of 89.7% (Table 2). The receiver operating characteristic curve of the test cohort is presented in Figure 3. The area under the curve (AUC) was 0.971. The specificity and AUC were calculated for the discrimination of breast cancer from the non‐cancer controls.
Affiliation: Department of Breast and Medical Oncology, National Cancer Center Hospital, Tokyo, Japan.