Cystatin C as a p53-inducible apoptotic mediator that regulates cathepsin L activity.
Bottom Line: We showed that cathepsin L activity was decreased in HCT116 p53(+/+) cells after adriamycin treatment, but not in HCT116 p53(-/-) cells.We also found that knockdown of cystatin C reduced adriamycin-induced caspase-3 activation.Cystatin C expression was significantly downregulated in breast cancer cells with p53 mutations, and decreased cystatin C expression was associated with poor prognosis of breast cancer.
Affiliation: Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Minato, Tokyo, Japan.Show MeSH
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Mentions: To further evaluate the induction of cystatin C by p53, U373MG and H1299 cells were infected with Ad‐p53 or Ad‐LacZ. We found that cystatin C mRNA and protein were induced in cells infected with Ad‐p53, indicating the regulation of cystatin C by p53 (Fig. 2a,b).
Affiliation: Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Minato, Tokyo, Japan.