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FOXA1 expression affects the proliferation activity of luminal breast cancer stem cell populations.

Tachi K, Shiraishi A, Bando H, Yamashita T, Tsuboi I, Kato T, Hara H, Ohneda O - Cancer Sci. (2016)

Bottom Line: Using a quantitative PCR analysis, we found that mammosphere forming cells showed a higher expression of FOXA1 and stemness-related genes compared with adherent culture cells.As a result, we found no significant difference in the number of mammospheres but decreased colony formation in shFOXA1 MCF-7 cells compared with control.These results suggest that the expression of FOXA1 appears to be involved in the proliferation of immature BC cells rather than the induction of stemness-related genes and self-renewal potency of CSCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast-Thyroid-Endocrine Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.

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Assessment of the potential of cancer stemness. (a) Aldehyde dehydrogenase (ALDH) activity of MCF‐7 (left), HCC1500 (middle), and BC#1 (right) cell lines. Black line, unstained sample; broken line, sample treated with N,N‐diethylaminobenzaldehyde (inhibitor) and ALDH reagent; black bold line, sample treated with ALDH reagent. The black bar and rate shows the positive area. (b) Morphology of MCF‐7 (left), HCC1500 (middle), and BC#1 (right) cells in mammosphere culture. Bar indicates 200 μm. (c) The number of mammospheres greater than 100 μm in diameter in each cell line. Black bar, HCC1500; gray bar, BC#1; white bar, MCF‐7. (d, e) ALDH activity in adherent culture samples (d) and mammosphere culture samples (e) of MCF‐7 cells. Black line, unstained sample; broken line, sample treated with N,N‐diethylaminobenzaldehyde and ALDH reagent; black bold line, sample treated with ALDH reagent. The black bar and rate shows the positive area.
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cas12870-fig-0002: Assessment of the potential of cancer stemness. (a) Aldehyde dehydrogenase (ALDH) activity of MCF‐7 (left), HCC1500 (middle), and BC#1 (right) cell lines. Black line, unstained sample; broken line, sample treated with N,N‐diethylaminobenzaldehyde (inhibitor) and ALDH reagent; black bold line, sample treated with ALDH reagent. The black bar and rate shows the positive area. (b) Morphology of MCF‐7 (left), HCC1500 (middle), and BC#1 (right) cells in mammosphere culture. Bar indicates 200 μm. (c) The number of mammospheres greater than 100 μm in diameter in each cell line. Black bar, HCC1500; gray bar, BC#1; white bar, MCF‐7. (d, e) ALDH activity in adherent culture samples (d) and mammosphere culture samples (e) of MCF‐7 cells. Black line, unstained sample; broken line, sample treated with N,N‐diethylaminobenzaldehyde and ALDH reagent; black bold line, sample treated with ALDH reagent. The black bar and rate shows the positive area.

Mentions: We evaluated the ALDH activity and frequency of CD44+/24− cells in our luminal BC cell lines (Figs 2a, S1). We found that BC#1 contains CSC populations at the highest frequency among the three BC cell lines investigated. The number of mammospheres was the highest in BC#1 compared to the other two BC cell lines (Fig. 2b and 2c). It has been previously reported that the changing of culture conditions from regular adherent culture to mammosphere culture led to the elevated expression of genes related to stemness.17 As shown in Figure 2(d,e), under a mammosphere culture, ALDH‐positive MCF‐7 cells increased compared to MCF‐7 cells cultured under adherent conditions.


FOXA1 expression affects the proliferation activity of luminal breast cancer stem cell populations.

Tachi K, Shiraishi A, Bando H, Yamashita T, Tsuboi I, Kato T, Hara H, Ohneda O - Cancer Sci. (2016)

Assessment of the potential of cancer stemness. (a) Aldehyde dehydrogenase (ALDH) activity of MCF‐7 (left), HCC1500 (middle), and BC#1 (right) cell lines. Black line, unstained sample; broken line, sample treated with N,N‐diethylaminobenzaldehyde (inhibitor) and ALDH reagent; black bold line, sample treated with ALDH reagent. The black bar and rate shows the positive area. (b) Morphology of MCF‐7 (left), HCC1500 (middle), and BC#1 (right) cells in mammosphere culture. Bar indicates 200 μm. (c) The number of mammospheres greater than 100 μm in diameter in each cell line. Black bar, HCC1500; gray bar, BC#1; white bar, MCF‐7. (d, e) ALDH activity in adherent culture samples (d) and mammosphere culture samples (e) of MCF‐7 cells. Black line, unstained sample; broken line, sample treated with N,N‐diethylaminobenzaldehyde and ALDH reagent; black bold line, sample treated with ALDH reagent. The black bar and rate shows the positive area.
© Copyright Policy - creativeCommonsBy-nc-nd
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4814260&req=5

cas12870-fig-0002: Assessment of the potential of cancer stemness. (a) Aldehyde dehydrogenase (ALDH) activity of MCF‐7 (left), HCC1500 (middle), and BC#1 (right) cell lines. Black line, unstained sample; broken line, sample treated with N,N‐diethylaminobenzaldehyde (inhibitor) and ALDH reagent; black bold line, sample treated with ALDH reagent. The black bar and rate shows the positive area. (b) Morphology of MCF‐7 (left), HCC1500 (middle), and BC#1 (right) cells in mammosphere culture. Bar indicates 200 μm. (c) The number of mammospheres greater than 100 μm in diameter in each cell line. Black bar, HCC1500; gray bar, BC#1; white bar, MCF‐7. (d, e) ALDH activity in adherent culture samples (d) and mammosphere culture samples (e) of MCF‐7 cells. Black line, unstained sample; broken line, sample treated with N,N‐diethylaminobenzaldehyde and ALDH reagent; black bold line, sample treated with ALDH reagent. The black bar and rate shows the positive area.
Mentions: We evaluated the ALDH activity and frequency of CD44+/24− cells in our luminal BC cell lines (Figs 2a, S1). We found that BC#1 contains CSC populations at the highest frequency among the three BC cell lines investigated. The number of mammospheres was the highest in BC#1 compared to the other two BC cell lines (Fig. 2b and 2c). It has been previously reported that the changing of culture conditions from regular adherent culture to mammosphere culture led to the elevated expression of genes related to stemness.17 As shown in Figure 2(d,e), under a mammosphere culture, ALDH‐positive MCF‐7 cells increased compared to MCF‐7 cells cultured under adherent conditions.

Bottom Line: Using a quantitative PCR analysis, we found that mammosphere forming cells showed a higher expression of FOXA1 and stemness-related genes compared with adherent culture cells.As a result, we found no significant difference in the number of mammospheres but decreased colony formation in shFOXA1 MCF-7 cells compared with control.These results suggest that the expression of FOXA1 appears to be involved in the proliferation of immature BC cells rather than the induction of stemness-related genes and self-renewal potency of CSCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast-Thyroid-Endocrine Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.

Show MeSH
Related in: MedlinePlus