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Invariant NKT cells are resistant to circulating CD15+ myeloid-derived suppressor cells in patients with head and neck cancer.

Horinaka A, Sakurai D, Ihara F, Makita Y, Kunii N, Motohashi S, Nakayama T, Okamoto Y - Cancer Sci. (2016)

Bottom Line: However, the effect on the host immune system, especially on invariant NKT (iNKT) cells with potent anti-tumor activity, remains unclear.However, iNKT cell activation upon α-galactosylceramide (αGalCer) stimulation was not affected by the presence or absence of CD15+ G-MDSC.Cancer immunotherapy designed to enhance the antitumor activity of iNKT cells by stimulation with αGalCer may remain effective in the presence of G-MDSC.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.

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Related in: MedlinePlus

Identification of monocytic‐myeloid‐derived suppressor cells (M‐MDSC) in peripheral blood cells. M‐MDSC were defined as the HLA‐DR−/low CD14+ cell population. Granulocytic‐myeloid‐derived suppressor cells (G‐MDSC) were defined as the HLA‐DR− Lin− CD14− CD15+ cell population. (a–c) Representative staining and gating of M‐MDSC and G‐MDSC in peripheral blood cells (PBC) from a healthy donor, M‐MDSC from a patient with head and neck squamous cell carcinoma (HNSCC) and G‐MDSC from a patient with HNSCC, respectively. The CD15+ fraction of G‐MDSC stained positive for CD11b+ and CD33+. (d) No significant correlation was seen between the percentages of M‐MDSC and G‐MDSC in HNSCC patients.
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cas12866-fig-0001: Identification of monocytic‐myeloid‐derived suppressor cells (M‐MDSC) in peripheral blood cells. M‐MDSC were defined as the HLA‐DR−/low CD14+ cell population. Granulocytic‐myeloid‐derived suppressor cells (G‐MDSC) were defined as the HLA‐DR− Lin− CD14− CD15+ cell population. (a–c) Representative staining and gating of M‐MDSC and G‐MDSC in peripheral blood cells (PBC) from a healthy donor, M‐MDSC from a patient with head and neck squamous cell carcinoma (HNSCC) and G‐MDSC from a patient with HNSCC, respectively. The CD15+ fraction of G‐MDSC stained positive for CD11b+ and CD33+. (d) No significant correlation was seen between the percentages of M‐MDSC and G‐MDSC in HNSCC patients.

Mentions: Compared with healthy donors, a significant increase in CD14+ HLA‐DR−/low M‐MDSC and HLA‐DR− Lin− CD15+ G‐MDSC was observed in HNSCC patients. No significant correlation was seen between the patient age and the ratio of M‐MDSC or G‐MDSC in HNSCC patients (data not shown). Representative staining profiles of M‐MDSC and G‐MDSC are shown (Fig. 1a–c). The CD15+ cells were also positive for both CD11b and CD33 (Fig. 1c). However, there was no correlation between the percentage of G‐MDSC and M‐MDSC (Fig. 1d).


Invariant NKT cells are resistant to circulating CD15+ myeloid-derived suppressor cells in patients with head and neck cancer.

Horinaka A, Sakurai D, Ihara F, Makita Y, Kunii N, Motohashi S, Nakayama T, Okamoto Y - Cancer Sci. (2016)

Identification of monocytic‐myeloid‐derived suppressor cells (M‐MDSC) in peripheral blood cells. M‐MDSC were defined as the HLA‐DR−/low CD14+ cell population. Granulocytic‐myeloid‐derived suppressor cells (G‐MDSC) were defined as the HLA‐DR− Lin− CD14− CD15+ cell population. (a–c) Representative staining and gating of M‐MDSC and G‐MDSC in peripheral blood cells (PBC) from a healthy donor, M‐MDSC from a patient with head and neck squamous cell carcinoma (HNSCC) and G‐MDSC from a patient with HNSCC, respectively. The CD15+ fraction of G‐MDSC stained positive for CD11b+ and CD33+. (d) No significant correlation was seen between the percentages of M‐MDSC and G‐MDSC in HNSCC patients.
© Copyright Policy - creativeCommonsBy-nc-nd
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4814259&req=5

cas12866-fig-0001: Identification of monocytic‐myeloid‐derived suppressor cells (M‐MDSC) in peripheral blood cells. M‐MDSC were defined as the HLA‐DR−/low CD14+ cell population. Granulocytic‐myeloid‐derived suppressor cells (G‐MDSC) were defined as the HLA‐DR− Lin− CD14− CD15+ cell population. (a–c) Representative staining and gating of M‐MDSC and G‐MDSC in peripheral blood cells (PBC) from a healthy donor, M‐MDSC from a patient with head and neck squamous cell carcinoma (HNSCC) and G‐MDSC from a patient with HNSCC, respectively. The CD15+ fraction of G‐MDSC stained positive for CD11b+ and CD33+. (d) No significant correlation was seen between the percentages of M‐MDSC and G‐MDSC in HNSCC patients.
Mentions: Compared with healthy donors, a significant increase in CD14+ HLA‐DR−/low M‐MDSC and HLA‐DR− Lin− CD15+ G‐MDSC was observed in HNSCC patients. No significant correlation was seen between the patient age and the ratio of M‐MDSC or G‐MDSC in HNSCC patients (data not shown). Representative staining profiles of M‐MDSC and G‐MDSC are shown (Fig. 1a–c). The CD15+ cells were also positive for both CD11b and CD33 (Fig. 1c). However, there was no correlation between the percentage of G‐MDSC and M‐MDSC (Fig. 1d).

Bottom Line: However, the effect on the host immune system, especially on invariant NKT (iNKT) cells with potent anti-tumor activity, remains unclear.However, iNKT cell activation upon α-galactosylceramide (αGalCer) stimulation was not affected by the presence or absence of CD15+ G-MDSC.Cancer immunotherapy designed to enhance the antitumor activity of iNKT cells by stimulation with αGalCer may remain effective in the presence of G-MDSC.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.

Show MeSH
Related in: MedlinePlus