Tumor growth suppression by the combination of nanobubbles and ultrasound.
Bottom Line: Tumor temperatures were significantly higher when treated with BL + ultrasound than ultrasound alone.In vivo depletion of CD8+ T cells (not NK or CD4+ T cells) completely blocked the effect of BL + ultrasound on tumor growth.Finally, we concluded that BL + ultrasound, which can prime the anti-tumor cellular immune system, may be an effective hyperthermia strategy for cancer treatment.
Affiliation: Laboratory of Drug Delivery System, Faculty of Pharma-Sciences, Teikyo University, Tokyo, Japan.Show MeSH
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Mentions: In vivo studies were conducted with BALB/c mice to test the impact of BL collapse on heat generation in the tumor during ultrasound exposure. Ten days after subcutaneous inoculation of Colon 26 tumor cells, the anesthetized animals were intratumorally injected with BL or saline, followed by ultrasound exposure. Figure 1a shows that tumor temperature gradually increased with ultrasound intensity. In contrast, tumor temperatures were significantly higher in the presence of intratumoral BL, and the overheating effect increased with ultrasound intensity. In the treatment of BL and 4 W/cm2 ultrasound exposures, the tumor temperature gradually increased and achieved the plateau at approximately 1.5 min. Finally, this tumor temperature at 2 min with BL + ultrasound was 6°C higher than with saline + ultrasound (Fig. 1b).
Affiliation: Laboratory of Drug Delivery System, Faculty of Pharma-Sciences, Teikyo University, Tokyo, Japan.