Establishment of a novel method to evaluate peritoneal microdissemination and therapeutic effect using luciferase assay.
Bottom Line: Effective anticancer agents and treatment protocols are necessary to improve outcomes in these patients.However, previous studies using mouse models of peritoneal dissemination have not detected any drug effect against peritoneal micrometastasis.Therefore, it is a useful model to validate peritoneal micrometastasis formation and to evaluate drug efficacy without killing mice.
Affiliation: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.Show MeSH
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Mentions: In in vivo luciferase assay, on day 10, luminescence was observed in all three of the surviving mice in the no treatment group, in three of the four mice in the CDDP group (Fig. 3a), and in three of the five mice in the gemcitabine group. Luminescence intensity in the no treatment group was significantly stronger than that in the CDDP and gemcitabine groups (Fig. 3b).
Affiliation: Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.