Antibody-dependent cellular cytotoxicity toward neuroblastoma enhanced by activated invariant natural killer T cells.
Bottom Line: For further improvement in patient outcomes, ways to maximize the cytotoxic effects of anti-GD2 therapies with minimal toxicity are required.Although some of the expanded iNKT cells expressed natural killer (NK) cell markers, including FcγR, iNKT cells were not directly associated with ADCC.In conclusion, iNKT cell-based immunotherapy could be an appropriate candidate for anti-GD2 antibody therapy for neuroblastoma.
Affiliation: Department of Medical Immunology, Graduate School of Medicine, Chiba University, Chiba, Japan.Show MeSH
Related in: MedlinePlus
Mentions: To assess the responsible cytokines for ADCC enhancement, neutralization of IFNγ, IL‐2, TNFα and GM‐CSF was performed. Using Transwell plates, NK cells were seeded in the lower well, iNKT cells and moDC were seeded in the upper well, and neutralizing mAbs or isotype controls were also added. NK cells were collected after 48 h, and cytotoxicity assays were performed using NMB NB cells with anti‐GD2 Abs. None of the neutralizing mAbs abrogated the cytotoxicity of NK cells activated by iNKT cells and αGalCer‐pulsed moDC (Fig. 7a–d).
Affiliation: Department of Medical Immunology, Graduate School of Medicine, Chiba University, Chiba, Japan.