Serum deprivation response inhibits breast cancer progression by blocking transforming growth factor-β signaling.
Bottom Line: Here, we found that SDPR is downregulated in human breast cancer.In conclusion, our results showed that SDPR inhibits breast cancer progression by blocking TGF-β signaling.SDPR depletion induces epithelial-mesenchymal transition by activation of TGF-β signaling.
Affiliation: The First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China.Show MeSH
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Mentions: Next, we investigated the influence of SDPR on breast cancer cell proliferation and invasion by generation of stable SDPR‐overexpressed MDA‐MB‐231 cell line (Fig. 2a). Overexpression of SDPR resulted in a lower proliferation rate in MDA‐MB‐231 cells as assessed by MTT (Fig. 2b), colony formation (Fig. 2c) and EdU (Fig. 2d) assays. Moreover, SDPR overexpression reduced cell invasion in MDA‐MB‐231 cells (Fig. 2e). We also observed that the apoptotic cells were significantly higher in SDPR‐overexpressed MDA‐MB‐231 cells (Fig. 2f).
Affiliation: The First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China.