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Serum deprivation response inhibits breast cancer progression by blocking transforming growth factor-β signaling.

Tian Y, Yu Y, Hou LK, Chi JR, Mao JF, Xia L, Wang X, Wang P, Cao XC - Cancer Sci. (2016)

Bottom Line: Here, we found that SDPR is downregulated in human breast cancer.In conclusion, our results showed that SDPR inhibits breast cancer progression by blocking TGF-β signaling.SDPR depletion induces epithelial-mesenchymal transition by activation of TGF-β signaling.

View Article: PubMed Central - PubMed

Affiliation: The First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China.

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Related in: MedlinePlus

Overexpression of serum deprivation response (SDPR) suppresses cell proliferation and invasion in MDA‐MB‐231 cell. (a) Western blot analysis of SDPR expression in 231‐SDPR cells compared with vector control cells. (b–d) Cell proliferation analyzed by MTT (b), colony formation (c) and EdU (d) in 231‐SDPR and vector control cells. (e) Transwell analysis of cell invasion in 231‐SDPR and vector control cells. (f) The percentage of apoptotic cells in 231‐SDPR and vector control cells. **P < 0.01, ***P < 0.001.
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cas12879-fig-0002: Overexpression of serum deprivation response (SDPR) suppresses cell proliferation and invasion in MDA‐MB‐231 cell. (a) Western blot analysis of SDPR expression in 231‐SDPR cells compared with vector control cells. (b–d) Cell proliferation analyzed by MTT (b), colony formation (c) and EdU (d) in 231‐SDPR and vector control cells. (e) Transwell analysis of cell invasion in 231‐SDPR and vector control cells. (f) The percentage of apoptotic cells in 231‐SDPR and vector control cells. **P < 0.01, ***P < 0.001.

Mentions: Next, we investigated the influence of SDPR on breast cancer cell proliferation and invasion by generation of stable SDPR‐overexpressed MDA‐MB‐231 cell line (Fig. 2a). Overexpression of SDPR resulted in a lower proliferation rate in MDA‐MB‐231 cells as assessed by MTT (Fig. 2b), colony formation (Fig. 2c) and EdU (Fig. 2d) assays. Moreover, SDPR overexpression reduced cell invasion in MDA‐MB‐231 cells (Fig. 2e). We also observed that the apoptotic cells were significantly higher in SDPR‐overexpressed MDA‐MB‐231 cells (Fig. 2f).


Serum deprivation response inhibits breast cancer progression by blocking transforming growth factor-β signaling.

Tian Y, Yu Y, Hou LK, Chi JR, Mao JF, Xia L, Wang X, Wang P, Cao XC - Cancer Sci. (2016)

Overexpression of serum deprivation response (SDPR) suppresses cell proliferation and invasion in MDA‐MB‐231 cell. (a) Western blot analysis of SDPR expression in 231‐SDPR cells compared with vector control cells. (b–d) Cell proliferation analyzed by MTT (b), colony formation (c) and EdU (d) in 231‐SDPR and vector control cells. (e) Transwell analysis of cell invasion in 231‐SDPR and vector control cells. (f) The percentage of apoptotic cells in 231‐SDPR and vector control cells. **P < 0.01, ***P < 0.001.
© Copyright Policy - creativeCommonsBy-nc-nd
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814251&req=5

cas12879-fig-0002: Overexpression of serum deprivation response (SDPR) suppresses cell proliferation and invasion in MDA‐MB‐231 cell. (a) Western blot analysis of SDPR expression in 231‐SDPR cells compared with vector control cells. (b–d) Cell proliferation analyzed by MTT (b), colony formation (c) and EdU (d) in 231‐SDPR and vector control cells. (e) Transwell analysis of cell invasion in 231‐SDPR and vector control cells. (f) The percentage of apoptotic cells in 231‐SDPR and vector control cells. **P < 0.01, ***P < 0.001.
Mentions: Next, we investigated the influence of SDPR on breast cancer cell proliferation and invasion by generation of stable SDPR‐overexpressed MDA‐MB‐231 cell line (Fig. 2a). Overexpression of SDPR resulted in a lower proliferation rate in MDA‐MB‐231 cells as assessed by MTT (Fig. 2b), colony formation (Fig. 2c) and EdU (Fig. 2d) assays. Moreover, SDPR overexpression reduced cell invasion in MDA‐MB‐231 cells (Fig. 2e). We also observed that the apoptotic cells were significantly higher in SDPR‐overexpressed MDA‐MB‐231 cells (Fig. 2f).

Bottom Line: Here, we found that SDPR is downregulated in human breast cancer.In conclusion, our results showed that SDPR inhibits breast cancer progression by blocking TGF-β signaling.SDPR depletion induces epithelial-mesenchymal transition by activation of TGF-β signaling.

View Article: PubMed Central - PubMed

Affiliation: The First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China.

Show MeSH
Related in: MedlinePlus