Early diagnosis of lymph node metastasis: Importance of intranodal pressures.
Bottom Line: We found that intranodal pressure in the subiliac lymph node increased at the stage when metastasis was detected by in vivo bioluminescence, but when proper axillary lymph node volume (measured by high-frequency ultrasound imaging) had not increased significantly.Intravenously injected liposomes, encapsulating indocyanine green, were detected in solid tumors by in vivo bioluminescence, but not in the proper axillary lymph node.Basic blood vessel and lymphatic channel structures were maintained in the proper axillary lymph node, although sinus histiocytosis was detected.
Affiliation: Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.Show MeSH
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Mentions: Figure 5 shows representative images of the SiLN and PALN stained with H&E, anti‐LYVE‐1 antibody, or anti‐CD31 antibody, under control conditions (day 3), or after inoculation with KM‐Luc/GFP (day 6) or FM3A‐Luc (day 14) cells. The basic internal structures (medulla, paracortex, cortex, and lymphatic channels) were preserved in tumor‐containing SiLN and metastasized PALN.4 Discrete blood vessels were observed in all LNs (Fig. 5A), and lymphatic sinus histiocytosis in the PALN and SiLN (Fig. 5B). Next, we measured LN vessel density (the CD31‐positive area) using immunofluorescence techniques (Fig. 6A). Blood vessel density did not differ between the control and metastasis groups (Fig. 6B,C). It has been noted that as mice age, their lymph node structures change over a period of several weeks. After 12 weeks, follicles tend to atrophy due to the accumulation of abnormal lymphocytes. We observed that the degree of atrophy was similar for control and metastatic lymph nodes, except for the portion of the lymph node comprising metastatic tumor.
Affiliation: Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.