Limits...
Early diagnosis of lymph node metastasis: Importance of intranodal pressures.

Miura Y, Mikada M, Ouchi T, Horie S, Takeda K, Yamaki T, Sakamoto M, Mori S, Kodama T - Cancer Sci. (2016)

Bottom Line: We found that intranodal pressure in the subiliac lymph node increased at the stage when metastasis was detected by in vivo bioluminescence, but when proper axillary lymph node volume (measured by high-frequency ultrasound imaging) had not increased significantly.Intravenously injected liposomes, encapsulating indocyanine green, were detected in solid tumors by in vivo bioluminescence, but not in the proper axillary lymph node.Basic blood vessel and lymphatic channel structures were maintained in the proper axillary lymph node, although sinus histiocytosis was detected.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.

Show MeSH

Related in: MedlinePlus

Induction of metastasis in a mouse model. (A–D) Changes in luciferase activity. (A,C) Luciferase activities induced by KM‐Luc/GFP cell injection into the subiliac lymph node (SiLN) (n = 24). Metastasis to the proper axillary lymph node (PALN) was detected on day 6. Luciferase activity increased in both the SiLN and PALN, but was higher in the SiLN. (B,D) Luciferase activities induced by FM3A‐Luc cell injection into the SiLN (n = 36). Metastasis to the PALN was detected on day 14. Tumor growth was slower than for KM‐Luc/GFP cells, but the trends in luciferase activity were similar. *P < 0.05, **P < 0.01 versus day 0 (one‐way anova and Tukey's test). Mean ± SD values are shown. (E–H) Changes in lymph node size. (E,G) Change in PALN volume, metastasized by KM‐Luc/GFP cells (n = 12). (F,H) Change in PALN volume, metastasized by FM3A‐Luc cells (n = 12). Lymph node volume was measured using high‐frequency ultrasound imaging. Mean ± SEM values are shown, normalized to those on day −1.
© Copyright Policy - creativeCommonsBy-nc-nd
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4814246&req=5

cas12873-fig-0002: Induction of metastasis in a mouse model. (A–D) Changes in luciferase activity. (A,C) Luciferase activities induced by KM‐Luc/GFP cell injection into the subiliac lymph node (SiLN) (n = 24). Metastasis to the proper axillary lymph node (PALN) was detected on day 6. Luciferase activity increased in both the SiLN and PALN, but was higher in the SiLN. (B,D) Luciferase activities induced by FM3A‐Luc cell injection into the SiLN (n = 36). Metastasis to the PALN was detected on day 14. Tumor growth was slower than for KM‐Luc/GFP cells, but the trends in luciferase activity were similar. *P < 0.05, **P < 0.01 versus day 0 (one‐way anova and Tukey's test). Mean ± SD values are shown. (E–H) Changes in lymph node size. (E,G) Change in PALN volume, metastasized by KM‐Luc/GFP cells (n = 12). (F,H) Change in PALN volume, metastasized by FM3A‐Luc cells (n = 12). Lymph node volume was measured using high‐frequency ultrasound imaging. Mean ± SEM values are shown, normalized to those on day −1.

Mentions: KM‐Luc/GFP or FM3A‐Luc cells were injected into the SiLN to provoke metastasis to the PALN. Both cell types expressed VEGF‐A and VEGF‐B but not VEGF‐C; KM‐Luc/GFP but not FM3A‐Luc cells showed slight VEGF‐D expression (Fig. 1D). Figure 2 shows the luciferase activity of KM‐Luc/GFP cells in the SiLN and PALN (days 0, 3, and 6; Fig. 2A,C), and that of FM3A‐Luc cells (days 0, 3, 6, 10, and 14; Fig. 2B,D). Luciferase activity in both LNs increased over time, with larger increases in the SiLN. Tumor cells were detected in the PALN on day 6 for KM‐Luc/GFP cells and day 14 for FM3A‐Luc cells. In subsequent experiments, INP measurements were made from the early stages of metastasis to day 6 (KM‐Luc/GFP) or day 14 (FM3A‐Luc).


Early diagnosis of lymph node metastasis: Importance of intranodal pressures.

Miura Y, Mikada M, Ouchi T, Horie S, Takeda K, Yamaki T, Sakamoto M, Mori S, Kodama T - Cancer Sci. (2016)

Induction of metastasis in a mouse model. (A–D) Changes in luciferase activity. (A,C) Luciferase activities induced by KM‐Luc/GFP cell injection into the subiliac lymph node (SiLN) (n = 24). Metastasis to the proper axillary lymph node (PALN) was detected on day 6. Luciferase activity increased in both the SiLN and PALN, but was higher in the SiLN. (B,D) Luciferase activities induced by FM3A‐Luc cell injection into the SiLN (n = 36). Metastasis to the PALN was detected on day 14. Tumor growth was slower than for KM‐Luc/GFP cells, but the trends in luciferase activity were similar. *P < 0.05, **P < 0.01 versus day 0 (one‐way anova and Tukey's test). Mean ± SD values are shown. (E–H) Changes in lymph node size. (E,G) Change in PALN volume, metastasized by KM‐Luc/GFP cells (n = 12). (F,H) Change in PALN volume, metastasized by FM3A‐Luc cells (n = 12). Lymph node volume was measured using high‐frequency ultrasound imaging. Mean ± SEM values are shown, normalized to those on day −1.
© Copyright Policy - creativeCommonsBy-nc-nd
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814246&req=5

cas12873-fig-0002: Induction of metastasis in a mouse model. (A–D) Changes in luciferase activity. (A,C) Luciferase activities induced by KM‐Luc/GFP cell injection into the subiliac lymph node (SiLN) (n = 24). Metastasis to the proper axillary lymph node (PALN) was detected on day 6. Luciferase activity increased in both the SiLN and PALN, but was higher in the SiLN. (B,D) Luciferase activities induced by FM3A‐Luc cell injection into the SiLN (n = 36). Metastasis to the PALN was detected on day 14. Tumor growth was slower than for KM‐Luc/GFP cells, but the trends in luciferase activity were similar. *P < 0.05, **P < 0.01 versus day 0 (one‐way anova and Tukey's test). Mean ± SD values are shown. (E–H) Changes in lymph node size. (E,G) Change in PALN volume, metastasized by KM‐Luc/GFP cells (n = 12). (F,H) Change in PALN volume, metastasized by FM3A‐Luc cells (n = 12). Lymph node volume was measured using high‐frequency ultrasound imaging. Mean ± SEM values are shown, normalized to those on day −1.
Mentions: KM‐Luc/GFP or FM3A‐Luc cells were injected into the SiLN to provoke metastasis to the PALN. Both cell types expressed VEGF‐A and VEGF‐B but not VEGF‐C; KM‐Luc/GFP but not FM3A‐Luc cells showed slight VEGF‐D expression (Fig. 1D). Figure 2 shows the luciferase activity of KM‐Luc/GFP cells in the SiLN and PALN (days 0, 3, and 6; Fig. 2A,C), and that of FM3A‐Luc cells (days 0, 3, 6, 10, and 14; Fig. 2B,D). Luciferase activity in both LNs increased over time, with larger increases in the SiLN. Tumor cells were detected in the PALN on day 6 for KM‐Luc/GFP cells and day 14 for FM3A‐Luc cells. In subsequent experiments, INP measurements were made from the early stages of metastasis to day 6 (KM‐Luc/GFP) or day 14 (FM3A‐Luc).

Bottom Line: We found that intranodal pressure in the subiliac lymph node increased at the stage when metastasis was detected by in vivo bioluminescence, but when proper axillary lymph node volume (measured by high-frequency ultrasound imaging) had not increased significantly.Intravenously injected liposomes, encapsulating indocyanine green, were detected in solid tumors by in vivo bioluminescence, but not in the proper axillary lymph node.Basic blood vessel and lymphatic channel structures were maintained in the proper axillary lymph node, although sinus histiocytosis was detected.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.

Show MeSH
Related in: MedlinePlus