Early diagnosis of lymph node metastasis: Importance of intranodal pressures.
Bottom Line: We found that intranodal pressure in the subiliac lymph node increased at the stage when metastasis was detected by in vivo bioluminescence, but when proper axillary lymph node volume (measured by high-frequency ultrasound imaging) had not increased significantly.Intravenously injected liposomes, encapsulating indocyanine green, were detected in solid tumors by in vivo bioluminescence, but not in the proper axillary lymph node.Basic blood vessel and lymphatic channel structures were maintained in the proper axillary lymph node, although sinus histiocytosis was detected.
Affiliation: Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.Show MeSH
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Mentions: KM‐Luc/GFP or FM3A‐Luc cells were injected into the SiLN to provoke metastasis to the PALN. Both cell types expressed VEGF‐A and VEGF‐B but not VEGF‐C; KM‐Luc/GFP but not FM3A‐Luc cells showed slight VEGF‐D expression (Fig. 1D). Figure 2 shows the luciferase activity of KM‐Luc/GFP cells in the SiLN and PALN (days 0, 3, and 6; Fig. 2A,C), and that of FM3A‐Luc cells (days 0, 3, 6, 10, and 14; Fig. 2B,D). Luciferase activity in both LNs increased over time, with larger increases in the SiLN. Tumor cells were detected in the PALN on day 6 for KM‐Luc/GFP cells and day 14 for FM3A‐Luc cells. In subsequent experiments, INP measurements were made from the early stages of metastasis to day 6 (KM‐Luc/GFP) or day 14 (FM3A‐Luc).
Affiliation: Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.