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Prognostic significance of CpG island methylator phenotype in surgically resected small cell lung carcinoma.

Saito Y, Nagae G, Motoi N, Miyauchi E, Ninomiya H, Uehara H, Mun M, Okumura S, Ohyanagi F, Nishio M, Satoh Y, Aburatani H, Ishikawa Y - Cancer Sci. (2016)

Bottom Line: Multivariate analyses revealed that postoperative chemotherapy and non-CIMP were significantly good prognostic factors.Ontology analyses suggested that the extrinsic apoptosis pathway was suppressed, including TNFRSF1A, TNFRSF10A and TRADD in CIMP tumors.Delineation of this phenotype may also be useful for the development of novel apoptosis-related chemotherapeutic agents for treatment of the aggressive tumor.

View Article: PubMed Central - PubMed

Affiliation: Division of Pathology, The Cancer Institute, Department of Pathology, The Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan.

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Clustering of 28 small cell lung cancer (SCLC) tumors by hierarchical clustering (a) and non‐negative matrix factorization (b). Consensus index values range from 0 to 1, with 0 being highly dissimilar and 1 being highly similar. Note that the independent two methods created exactly the same two clusters (Clusters 1 and 2).
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cas12876-fig-0003: Clustering of 28 small cell lung cancer (SCLC) tumors by hierarchical clustering (a) and non‐negative matrix factorization (b). Consensus index values range from 0 to 1, with 0 being highly dissimilar and 1 being highly similar. Note that the independent two methods created exactly the same two clusters (Clusters 1 and 2).

Mentions: Next, to identify SCLC subgroups, we used the 1741 probes with an SD of mean β‐value in tumor tissues >0.2 on each site and performed both the unsupervised hierarchical clustering (Fig. 3a) and the NMF (Fig. 3b). We identified two clusters with different methylation levels: Cluster 1 (n = 9) and Cluster 2 (n = 19). As shown in Figure 3(a,b), the two clusters created by both the methods were exactly the same, implying that the clusters were very robust. Cluster 1 tumors were identified as SCLC CIMP and Cluster 2 was non‐CIMP, because the CpG islands of Cluster 1 tumors were significantly hypermethylated as compared with Cluster 2 tumors (Fig. 4).


Prognostic significance of CpG island methylator phenotype in surgically resected small cell lung carcinoma.

Saito Y, Nagae G, Motoi N, Miyauchi E, Ninomiya H, Uehara H, Mun M, Okumura S, Ohyanagi F, Nishio M, Satoh Y, Aburatani H, Ishikawa Y - Cancer Sci. (2016)

Clustering of 28 small cell lung cancer (SCLC) tumors by hierarchical clustering (a) and non‐negative matrix factorization (b). Consensus index values range from 0 to 1, with 0 being highly dissimilar and 1 being highly similar. Note that the independent two methods created exactly the same two clusters (Clusters 1 and 2).
© Copyright Policy - creativeCommonsBy-nc
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814245&req=5

cas12876-fig-0003: Clustering of 28 small cell lung cancer (SCLC) tumors by hierarchical clustering (a) and non‐negative matrix factorization (b). Consensus index values range from 0 to 1, with 0 being highly dissimilar and 1 being highly similar. Note that the independent two methods created exactly the same two clusters (Clusters 1 and 2).
Mentions: Next, to identify SCLC subgroups, we used the 1741 probes with an SD of mean β‐value in tumor tissues >0.2 on each site and performed both the unsupervised hierarchical clustering (Fig. 3a) and the NMF (Fig. 3b). We identified two clusters with different methylation levels: Cluster 1 (n = 9) and Cluster 2 (n = 19). As shown in Figure 3(a,b), the two clusters created by both the methods were exactly the same, implying that the clusters were very robust. Cluster 1 tumors were identified as SCLC CIMP and Cluster 2 was non‐CIMP, because the CpG islands of Cluster 1 tumors were significantly hypermethylated as compared with Cluster 2 tumors (Fig. 4).

Bottom Line: Multivariate analyses revealed that postoperative chemotherapy and non-CIMP were significantly good prognostic factors.Ontology analyses suggested that the extrinsic apoptosis pathway was suppressed, including TNFRSF1A, TNFRSF10A and TRADD in CIMP tumors.Delineation of this phenotype may also be useful for the development of novel apoptosis-related chemotherapeutic agents for treatment of the aggressive tumor.

View Article: PubMed Central - PubMed

Affiliation: Division of Pathology, The Cancer Institute, Department of Pathology, The Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus