Wnt5a-Ror2 signaling in mesenchymal stem cells promotes proliferation of gastric cancer cells by activating CXCL16-CXCR6 axis.
Bottom Line: Wnt5a-Ror2 signaling has been shown to play important roles in promoting aggressiveness of various cancer cells in a cell-autonomous manner.However, little is known about its function in cancer-associated stromal cells, including mesenchymal stem cells (MSCs).Interestingly, it was found that expression of CXCL16 in MSCs is augmented by Wnt5a-Ror2 signaling, and that recombinant chemokine (C-X-C motif) ligand (CXCL)16 protein can enhance proliferation of MKN45 cells in the absence of MSCs.
Affiliation: Division of Cell Physiology, Department of Physiology and Cell Biology, Graduate School of Medicine, Kobe University, Kobe, Japan.Show MeSH
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Mentions: It was found that expression levels of both Ror2 and Wnt5a were relatively high in MSCs, but were marginal if any in MKN45‐Luc cells (Fig. S2). Thus, we then examined a possible role of Wnt5a‐Ror2 signaling in MSCs in promoting proliferation of MKN45‐Luc cells. Suppressed expression of Ror2 or Wnt5a in MSCs resulted in the inhibition of promoted proliferation of MKN45‐Luc cells by direct or indirect coculture with MSCs (Figs 2,S3). Furthermore, proliferation and viability of MSCs were unaffected by suppressed expression of Ror2 in the cells as assessed by WST assay (Fig. S4a). These results suggest that Wnt5a‐Ror2 signaling in MSCs might play a role in promoting proliferation of MKN45 cells.
Affiliation: Division of Cell Physiology, Department of Physiology and Cell Biology, Graduate School of Medicine, Kobe University, Kobe, Japan.