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Identification and Fine Mapping of a White Husk Gene in Barley (Hordeum vulgare L.).

Hua W, Zhang XQ, Zhu J, Shang Y, Wang J, Jia Q, Zhang Q, Yang J, Li C - PLoS ONE (2016)

Bottom Line: The diastatic power and α-amino nitrogen contents also significantly increased in wh mutant.However, the wlp1 orthologous gene was mapped outside the interval.New candidate genes were identified based on the barley genome sequence.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

ABSTRACT
Barley is the only crop in the Poaceae family with adhering husks at maturity. The color of husk at barely development stage could influence the agronomic traits and malting qualities of grains. A barley mutant with a white husk was discovered from the malting barley cultivar Supi 3 and designated wh (white husk). Morphological changes and the genetics of white husk barley were investigated. Husks of the mutant were white at the heading and flowering stages but yellowed at maturity. The diastatic power and α-amino nitrogen contents also significantly increased in wh mutant. Transmission electron microscopy examination showed abnormal chloroplast development in the mutant. Genetic analysis of F2 and BC1F1 populations developed from a cross of wh and Yangnongpi 5 (green husk) showed that the white husk was controlled by a single recessive gene (wh). The wh gene was initially mapped between 49.64 and 51.77 cM on chromosome 3H, which is syntenic with rice chromosome 1 where a white husk gene wlp1 has been isolated. The barley orthologous gene of wlp1 was sequenced from both parents and a 688 bp deletion identified in the wh mutant. We further fine-mapped the wh gene between SSR markers Bmac0067 and Bmag0508a with distances of 0.36 cM and 0.27 cM in an F2 population with 1115 individuals of white husk. However, the wlp1 orthologous gene was mapped outside the interval. New candidate genes were identified based on the barley genome sequence.

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Related in: MedlinePlus

Phenotype of wild-type Supi 3 and mutant wh.(A) spikes, (B) leaf, and (C) whole plant in the filling stage. Supi 3 on the left and wh on the right. The bar represents 1 cm.
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pone.0152128.g001: Phenotype of wild-type Supi 3 and mutant wh.(A) spikes, (B) leaf, and (C) whole plant in the filling stage. Supi 3 on the left and wh on the right. The bar represents 1 cm.

Mentions: In 2006, a mutant was discovered in a field of malting barley cultivar Supi 3, a leading cultivar in the Jiangsu Province, China. The husks in the mutant were colorless at the heading and flowering stages (Fig 1A) and became yellow but slightly brighter than Supi 3 in the mature grains. Husk color of mature grains of Supi 3 and wh were measured with a Konica Minolta CM-700d over two consecutive years; the mutant values for Min L (brightness) were 2.4 units higher than Supi 3 (Table 1), indicating brighter grains. Multi-year and -location experiments showed that the white husk trait is stably inherited.


Identification and Fine Mapping of a White Husk Gene in Barley (Hordeum vulgare L.).

Hua W, Zhang XQ, Zhu J, Shang Y, Wang J, Jia Q, Zhang Q, Yang J, Li C - PLoS ONE (2016)

Phenotype of wild-type Supi 3 and mutant wh.(A) spikes, (B) leaf, and (C) whole plant in the filling stage. Supi 3 on the left and wh on the right. The bar represents 1 cm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814061&req=5

pone.0152128.g001: Phenotype of wild-type Supi 3 and mutant wh.(A) spikes, (B) leaf, and (C) whole plant in the filling stage. Supi 3 on the left and wh on the right. The bar represents 1 cm.
Mentions: In 2006, a mutant was discovered in a field of malting barley cultivar Supi 3, a leading cultivar in the Jiangsu Province, China. The husks in the mutant were colorless at the heading and flowering stages (Fig 1A) and became yellow but slightly brighter than Supi 3 in the mature grains. Husk color of mature grains of Supi 3 and wh were measured with a Konica Minolta CM-700d over two consecutive years; the mutant values for Min L (brightness) were 2.4 units higher than Supi 3 (Table 1), indicating brighter grains. Multi-year and -location experiments showed that the white husk trait is stably inherited.

Bottom Line: The diastatic power and α-amino nitrogen contents also significantly increased in wh mutant.However, the wlp1 orthologous gene was mapped outside the interval.New candidate genes were identified based on the barley genome sequence.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

ABSTRACT
Barley is the only crop in the Poaceae family with adhering husks at maturity. The color of husk at barely development stage could influence the agronomic traits and malting qualities of grains. A barley mutant with a white husk was discovered from the malting barley cultivar Supi 3 and designated wh (white husk). Morphological changes and the genetics of white husk barley were investigated. Husks of the mutant were white at the heading and flowering stages but yellowed at maturity. The diastatic power and α-amino nitrogen contents also significantly increased in wh mutant. Transmission electron microscopy examination showed abnormal chloroplast development in the mutant. Genetic analysis of F2 and BC1F1 populations developed from a cross of wh and Yangnongpi 5 (green husk) showed that the white husk was controlled by a single recessive gene (wh). The wh gene was initially mapped between 49.64 and 51.77 cM on chromosome 3H, which is syntenic with rice chromosome 1 where a white husk gene wlp1 has been isolated. The barley orthologous gene of wlp1 was sequenced from both parents and a 688 bp deletion identified in the wh mutant. We further fine-mapped the wh gene between SSR markers Bmac0067 and Bmag0508a with distances of 0.36 cM and 0.27 cM in an F2 population with 1115 individuals of white husk. However, the wlp1 orthologous gene was mapped outside the interval. New candidate genes were identified based on the barley genome sequence.

Show MeSH
Related in: MedlinePlus