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Effects of a Closed Space Environment on Gene Expression in Hair Follicles of Astronauts in the International Space Station.

Terada M, Seki M, Takahashi R, Yamada S, Higashibata A, Majima HJ, Sudoh M, Mukai C, Ishioka N - PLoS ONE (2016)

Bottom Line: In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses.We found that spaceflight alters human hair follicle gene expression.The degree of changes in gene expression was found to vary among individuals.

View Article: PubMed Central - PubMed

Affiliation: Divison of Aerospace Medicine, The Jikei University School of Medicine, Minato-ku, Tokyo, Japan.

ABSTRACT
Adaptation to the space environment can sometimes pose physiological problems to International Space Station (ISS) astronauts after their return to earth. Therefore, it is important to develop healthcare technologies for astronauts. In this study, we examined the feasibility of using hair follicles, a readily obtained sample, to assess gene expression changes in response to spaceflight adaptation. In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses. We found that spaceflight alters human hair follicle gene expression. The degree of changes in gene expression was found to vary among individuals. In some astronauts, genes related to hair growth such as FGF18, ANGPTL7 and COMP were upregulated during flight, suggesting that spaceflight inhibits cell proliferation in hair follicles.

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Expression of hair follicle genes in each astronaut.Several genes were selected from the data depicted in Fig 3. Data from astronauts with high variability in gene expression. Small letters indicated sampling timepoints: (a) Preflight 1, (b) Preflight 2, (c) Inflight 1, (d) Inflight 2, (e) Postflight 1, (f) Postflight 2.
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pone.0150801.g005: Expression of hair follicle genes in each astronaut.Several genes were selected from the data depicted in Fig 3. Data from astronauts with high variability in gene expression. Small letters indicated sampling timepoints: (a) Preflight 1, (b) Preflight 2, (c) Inflight 1, (d) Inflight 2, (e) Postflight 1, (f) Postflight 2.

Mentions: A subset of genes from Table 3 was selected and corresponding microarray gene expression values were compared among subjects. In the bulge portion, four genes were selected: SERPINF1, FST, ANGPTL2, and DCN, and from the sub-bulge portion, six genes were chosen: TIMP3, ANGPIL7, PDGFC, COMP, MCAM, and FGF18. Two genes (NES and TNC) were selected from the marker genes of human epithelial hair follicle stem cells (immunophenotype genes). A comparison of these genes for each subject (subject number 1–10) is shown in Figs 4 and 5. Fig 4 shows the data from three astronauts, which shows an increase in gene expression during spaceflight. Fig 5 shows the data from other seven astronauts. Although the data from female astronauts (subject number 3 and 4) seemed relatively stable compared to the data from male astronauts (Fig 3), whose patterns showed high variability during all sampling points.


Effects of a Closed Space Environment on Gene Expression in Hair Follicles of Astronauts in the International Space Station.

Terada M, Seki M, Takahashi R, Yamada S, Higashibata A, Majima HJ, Sudoh M, Mukai C, Ishioka N - PLoS ONE (2016)

Expression of hair follicle genes in each astronaut.Several genes were selected from the data depicted in Fig 3. Data from astronauts with high variability in gene expression. Small letters indicated sampling timepoints: (a) Preflight 1, (b) Preflight 2, (c) Inflight 1, (d) Inflight 2, (e) Postflight 1, (f) Postflight 2.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814050&req=5

pone.0150801.g005: Expression of hair follicle genes in each astronaut.Several genes were selected from the data depicted in Fig 3. Data from astronauts with high variability in gene expression. Small letters indicated sampling timepoints: (a) Preflight 1, (b) Preflight 2, (c) Inflight 1, (d) Inflight 2, (e) Postflight 1, (f) Postflight 2.
Mentions: A subset of genes from Table 3 was selected and corresponding microarray gene expression values were compared among subjects. In the bulge portion, four genes were selected: SERPINF1, FST, ANGPTL2, and DCN, and from the sub-bulge portion, six genes were chosen: TIMP3, ANGPIL7, PDGFC, COMP, MCAM, and FGF18. Two genes (NES and TNC) were selected from the marker genes of human epithelial hair follicle stem cells (immunophenotype genes). A comparison of these genes for each subject (subject number 1–10) is shown in Figs 4 and 5. Fig 4 shows the data from three astronauts, which shows an increase in gene expression during spaceflight. Fig 5 shows the data from other seven astronauts. Although the data from female astronauts (subject number 3 and 4) seemed relatively stable compared to the data from male astronauts (Fig 3), whose patterns showed high variability during all sampling points.

Bottom Line: In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses.We found that spaceflight alters human hair follicle gene expression.The degree of changes in gene expression was found to vary among individuals.

View Article: PubMed Central - PubMed

Affiliation: Divison of Aerospace Medicine, The Jikei University School of Medicine, Minato-ku, Tokyo, Japan.

ABSTRACT
Adaptation to the space environment can sometimes pose physiological problems to International Space Station (ISS) astronauts after their return to earth. Therefore, it is important to develop healthcare technologies for astronauts. In this study, we examined the feasibility of using hair follicles, a readily obtained sample, to assess gene expression changes in response to spaceflight adaptation. In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses. We found that spaceflight alters human hair follicle gene expression. The degree of changes in gene expression was found to vary among individuals. In some astronauts, genes related to hair growth such as FGF18, ANGPTL7 and COMP were upregulated during flight, suggesting that spaceflight inhibits cell proliferation in hair follicles.

Show MeSH
Related in: MedlinePlus