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Effects of a Closed Space Environment on Gene Expression in Hair Follicles of Astronauts in the International Space Station.

Terada M, Seki M, Takahashi R, Yamada S, Higashibata A, Majima HJ, Sudoh M, Mukai C, Ishioka N - PLoS ONE (2016)

Bottom Line: In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses.We found that spaceflight alters human hair follicle gene expression.The degree of changes in gene expression was found to vary among individuals.

View Article: PubMed Central - PubMed

Affiliation: Divison of Aerospace Medicine, The Jikei University School of Medicine, Minato-ku, Tokyo, Japan.

ABSTRACT
Adaptation to the space environment can sometimes pose physiological problems to International Space Station (ISS) astronauts after their return to earth. Therefore, it is important to develop healthcare technologies for astronauts. In this study, we examined the feasibility of using hair follicles, a readily obtained sample, to assess gene expression changes in response to spaceflight adaptation. In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses. We found that spaceflight alters human hair follicle gene expression. The degree of changes in gene expression was found to vary among individuals. In some astronauts, genes related to hair growth such as FGF18, ANGPTL7 and COMP were upregulated during flight, suggesting that spaceflight inhibits cell proliferation in hair follicles.

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Expression of hair follicle genes in 10 astronauts.Gene expression (microarray) data from 10 astronauts. The number headings indicate subject ID number. Small letters indicate sampling time point: (a) Preflight 1, (b) Preflight 2, (c) Inflight 1, (d) Inflight 2, (e) Postflight 1, (f) Postflight 2. (A) Genes associated with hair bulge. (B) Genes associated with hair sub-bulge. (C) Marker genes of human epithelial hair follicle stem cells (immunophenotype genes).
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pone.0150801.g003: Expression of hair follicle genes in 10 astronauts.Gene expression (microarray) data from 10 astronauts. The number headings indicate subject ID number. Small letters indicate sampling time point: (a) Preflight 1, (b) Preflight 2, (c) Inflight 1, (d) Inflight 2, (e) Postflight 1, (f) Postflight 2. (A) Genes associated with hair bulge. (B) Genes associated with hair sub-bulge. (C) Marker genes of human epithelial hair follicle stem cells (immunophenotype genes).

Mentions: To confirm the integrity of RNA extracted from hair, expression levels of hair-specific genes in these samples were analyzed. The average value of first preflight and second preflight sampling points was used as baseline. Hair follicle specific genes were chosen based on the studies by Ohyama et al. [30] and Kloepper et al. [31]. The changes in the expression of 14 reported genes (Table 3A) in the bulge region of hair follicles were observed in the microarray data at all sampling points (Fig 3A). Similarly, the expression patterns of 22 genes in the hair sub-bulge region (Table 3B) were confirmed (Fig 3B). In addition, the expression patterns of 16 marker genes of human epithelial hair follicle stem cells (referred to as “immunophenotype genes” by Kloepper et al.) [31] (Table 3C) were detected (Fig 3C).


Effects of a Closed Space Environment on Gene Expression in Hair Follicles of Astronauts in the International Space Station.

Terada M, Seki M, Takahashi R, Yamada S, Higashibata A, Majima HJ, Sudoh M, Mukai C, Ishioka N - PLoS ONE (2016)

Expression of hair follicle genes in 10 astronauts.Gene expression (microarray) data from 10 astronauts. The number headings indicate subject ID number. Small letters indicate sampling time point: (a) Preflight 1, (b) Preflight 2, (c) Inflight 1, (d) Inflight 2, (e) Postflight 1, (f) Postflight 2. (A) Genes associated with hair bulge. (B) Genes associated with hair sub-bulge. (C) Marker genes of human epithelial hair follicle stem cells (immunophenotype genes).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814050&req=5

pone.0150801.g003: Expression of hair follicle genes in 10 astronauts.Gene expression (microarray) data from 10 astronauts. The number headings indicate subject ID number. Small letters indicate sampling time point: (a) Preflight 1, (b) Preflight 2, (c) Inflight 1, (d) Inflight 2, (e) Postflight 1, (f) Postflight 2. (A) Genes associated with hair bulge. (B) Genes associated with hair sub-bulge. (C) Marker genes of human epithelial hair follicle stem cells (immunophenotype genes).
Mentions: To confirm the integrity of RNA extracted from hair, expression levels of hair-specific genes in these samples were analyzed. The average value of first preflight and second preflight sampling points was used as baseline. Hair follicle specific genes were chosen based on the studies by Ohyama et al. [30] and Kloepper et al. [31]. The changes in the expression of 14 reported genes (Table 3A) in the bulge region of hair follicles were observed in the microarray data at all sampling points (Fig 3A). Similarly, the expression patterns of 22 genes in the hair sub-bulge region (Table 3B) were confirmed (Fig 3B). In addition, the expression patterns of 16 marker genes of human epithelial hair follicle stem cells (referred to as “immunophenotype genes” by Kloepper et al.) [31] (Table 3C) were detected (Fig 3C).

Bottom Line: In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses.We found that spaceflight alters human hair follicle gene expression.The degree of changes in gene expression was found to vary among individuals.

View Article: PubMed Central - PubMed

Affiliation: Divison of Aerospace Medicine, The Jikei University School of Medicine, Minato-ku, Tokyo, Japan.

ABSTRACT
Adaptation to the space environment can sometimes pose physiological problems to International Space Station (ISS) astronauts after their return to earth. Therefore, it is important to develop healthcare technologies for astronauts. In this study, we examined the feasibility of using hair follicles, a readily obtained sample, to assess gene expression changes in response to spaceflight adaptation. In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses. We found that spaceflight alters human hair follicle gene expression. The degree of changes in gene expression was found to vary among individuals. In some astronauts, genes related to hair growth such as FGF18, ANGPTL7 and COMP were upregulated during flight, suggesting that spaceflight inhibits cell proliferation in hair follicles.

Show MeSH
Related in: MedlinePlus