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Effects of a Closed Space Environment on Gene Expression in Hair Follicles of Astronauts in the International Space Station.

Terada M, Seki M, Takahashi R, Yamada S, Higashibata A, Majima HJ, Sudoh M, Mukai C, Ishioka N - PLoS ONE (2016)

Bottom Line: In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses.We found that spaceflight alters human hair follicle gene expression.The degree of changes in gene expression was found to vary among individuals.

View Article: PubMed Central - PubMed

Affiliation: Divison of Aerospace Medicine, The Jikei University School of Medicine, Minato-ku, Tokyo, Japan.

ABSTRACT
Adaptation to the space environment can sometimes pose physiological problems to International Space Station (ISS) astronauts after their return to earth. Therefore, it is important to develop healthcare technologies for astronauts. In this study, we examined the feasibility of using hair follicles, a readily obtained sample, to assess gene expression changes in response to spaceflight adaptation. In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses. We found that spaceflight alters human hair follicle gene expression. The degree of changes in gene expression was found to vary among individuals. In some astronauts, genes related to hair growth such as FGF18, ANGPTL7 and COMP were upregulated during flight, suggesting that spaceflight inhibits cell proliferation in hair follicles.

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Experiment schedule.At each sampling point, two astronauts were paired and each astronaut took hair samples from the other astronaut by turns (sample number indicated in the text). L: Launch, R: Return.
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pone.0150801.g001: Experiment schedule.At each sampling point, two astronauts were paired and each astronaut took hair samples from the other astronaut by turns (sample number indicated in the text). L: Launch, R: Return.

Mentions: Ten astronauts (male = 8, female = 2) at the International Space Station (ISS) participated in the study. They were at ISS on a 6-month-long mission. In each mission, five strands of hair were sampled six times from each astronaut within the period of July 2009 to February 2013. The sampling timings were as follows: 6 sampling time points (Fig 1); first preflight (Launch (L) -180 to -90 days: 6 to 3 months before launch), second preflight (L-60 to -14: 2 months to 2 weeks before launch), first inflight (L+20 to 37: 20 to 37 days after launch), second inflight (Return (R) -20 to -7: 20 to 7 days before return), first postflight (R+2 to 7: 2 to 7 days after return), and second postflight (R+30 to 90: from 1 to 3 months after return). The sampling days differed for each astronaut because of differing schedules. In each mission, two astronauts were paired and individual hair samples were collected. At one time point, five strands of hairs were grasped as close as possible to the scalp and pulled out using tweezers in the direction of hair growth without damaging the hair follicles. The samples were stored at -80°C until analysis at the preflight and postflight sampling points. In space, the samples were stored at Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as soon as possible until return.


Effects of a Closed Space Environment on Gene Expression in Hair Follicles of Astronauts in the International Space Station.

Terada M, Seki M, Takahashi R, Yamada S, Higashibata A, Majima HJ, Sudoh M, Mukai C, Ishioka N - PLoS ONE (2016)

Experiment schedule.At each sampling point, two astronauts were paired and each astronaut took hair samples from the other astronaut by turns (sample number indicated in the text). L: Launch, R: Return.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814050&req=5

pone.0150801.g001: Experiment schedule.At each sampling point, two astronauts were paired and each astronaut took hair samples from the other astronaut by turns (sample number indicated in the text). L: Launch, R: Return.
Mentions: Ten astronauts (male = 8, female = 2) at the International Space Station (ISS) participated in the study. They were at ISS on a 6-month-long mission. In each mission, five strands of hair were sampled six times from each astronaut within the period of July 2009 to February 2013. The sampling timings were as follows: 6 sampling time points (Fig 1); first preflight (Launch (L) -180 to -90 days: 6 to 3 months before launch), second preflight (L-60 to -14: 2 months to 2 weeks before launch), first inflight (L+20 to 37: 20 to 37 days after launch), second inflight (Return (R) -20 to -7: 20 to 7 days before return), first postflight (R+2 to 7: 2 to 7 days after return), and second postflight (R+30 to 90: from 1 to 3 months after return). The sampling days differed for each astronaut because of differing schedules. In each mission, two astronauts were paired and individual hair samples were collected. At one time point, five strands of hairs were grasped as close as possible to the scalp and pulled out using tweezers in the direction of hair growth without damaging the hair follicles. The samples were stored at -80°C until analysis at the preflight and postflight sampling points. In space, the samples were stored at Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as soon as possible until return.

Bottom Line: In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses.We found that spaceflight alters human hair follicle gene expression.The degree of changes in gene expression was found to vary among individuals.

View Article: PubMed Central - PubMed

Affiliation: Divison of Aerospace Medicine, The Jikei University School of Medicine, Minato-ku, Tokyo, Japan.

ABSTRACT
Adaptation to the space environment can sometimes pose physiological problems to International Space Station (ISS) astronauts after their return to earth. Therefore, it is important to develop healthcare technologies for astronauts. In this study, we examined the feasibility of using hair follicles, a readily obtained sample, to assess gene expression changes in response to spaceflight adaptation. In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses. We found that spaceflight alters human hair follicle gene expression. The degree of changes in gene expression was found to vary among individuals. In some astronauts, genes related to hair growth such as FGF18, ANGPTL7 and COMP were upregulated during flight, suggesting that spaceflight inhibits cell proliferation in hair follicles.

Show MeSH
Related in: MedlinePlus