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Copper-Containing Anti-Biofilm Nanofiber Scaffolds as a Wound Dressing Material.

Ahire JJ, Hattingh M, Neveling DP, Dicks LM - PLoS ONE (2016)

Bottom Line: Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter).The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC).After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.

ABSTRACT
Copper particles were incorporated into nanofibers during the electrospinning of poly-D,L-lactide (PDLLA) and poly(ethylene oxide) (PEO). The ability of the nanofibers to prevent Pseudomonas aeruginosa PA01 and Staphylococcus aureus (strain Xen 30) to form biofilms was tested. Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter). The crystalline structure of the copper particles in Cu-F was confirmed by X-ray diffraction (XRD). Copper crystals were encapsulated, but also attached to the surface of Cu-F, as shown scanning transmission electron microscopy (STEM) and transmission electron microscopy (TEM), respectively. The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC). After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively. Reduction in biofilm formation was ascribed to copper released from the nanofibers. Copper-containing nanofibers may be incorporated into wound dressings.

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Scanning electron microscopy (SEM) images of nanofibers collected from 48-h-old biofilms.Images (a) and (c) show cell growth of P. aeruginosa PA01 and S. aureus Xen 30, respectively, on the surface of CF. Images (b) and (d) show cell growth of P. aeruginosa PA01 and S. aureus Xen 30, respectively, on the surface of Cu-F.
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pone.0152755.g006: Scanning electron microscopy (SEM) images of nanofibers collected from 48-h-old biofilms.Images (a) and (c) show cell growth of P. aeruginosa PA01 and S. aureus Xen 30, respectively, on the surface of CF. Images (b) and (d) show cell growth of P. aeruginosa PA01 and S. aureus Xen 30, respectively, on the surface of Cu-F.

Mentions: Viable cell numbers recorded for P. aeruginosa PA01 and S. aureus Xen 30 were after 48 h approximately 5% less in the presence of CF, compared to cells grown in the absence of nanofibers (Fig 5A and 5C, respectively). The decline in cell numbers is attributed to adhesion of the two strains to nanofibers (CF), as shown in Fig 6A and 6C. Growth of P. aeruginosa PA01 declined with approximately 13% in the presence of Cu-F (Fig 5A) and growth of S. aureus Xen 30 with approximately 31% (Fig 5C). After 48 h in the presence of Cu-F, biofilm formation of P. aeruginosa PA01 decreased with approximately 41% (Fig 5B) and that of S. aureus Xen 30 with approximately 50% (Fig 5D), compared to biofilm formation in the absence of nanofibers and copper. Very few cells of P. aeruginosa PA01 and S. aureus Xen 30 adhered to Cu-F (Fig 6B and 6D). Based on these findings, copper that diffused from Cu-F inhibited cell growth and biofilm formation of S. aureus Xen 30 and P. aeruginosa PA01.


Copper-Containing Anti-Biofilm Nanofiber Scaffolds as a Wound Dressing Material.

Ahire JJ, Hattingh M, Neveling DP, Dicks LM - PLoS ONE (2016)

Scanning electron microscopy (SEM) images of nanofibers collected from 48-h-old biofilms.Images (a) and (c) show cell growth of P. aeruginosa PA01 and S. aureus Xen 30, respectively, on the surface of CF. Images (b) and (d) show cell growth of P. aeruginosa PA01 and S. aureus Xen 30, respectively, on the surface of Cu-F.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814046&req=5

pone.0152755.g006: Scanning electron microscopy (SEM) images of nanofibers collected from 48-h-old biofilms.Images (a) and (c) show cell growth of P. aeruginosa PA01 and S. aureus Xen 30, respectively, on the surface of CF. Images (b) and (d) show cell growth of P. aeruginosa PA01 and S. aureus Xen 30, respectively, on the surface of Cu-F.
Mentions: Viable cell numbers recorded for P. aeruginosa PA01 and S. aureus Xen 30 were after 48 h approximately 5% less in the presence of CF, compared to cells grown in the absence of nanofibers (Fig 5A and 5C, respectively). The decline in cell numbers is attributed to adhesion of the two strains to nanofibers (CF), as shown in Fig 6A and 6C. Growth of P. aeruginosa PA01 declined with approximately 13% in the presence of Cu-F (Fig 5A) and growth of S. aureus Xen 30 with approximately 31% (Fig 5C). After 48 h in the presence of Cu-F, biofilm formation of P. aeruginosa PA01 decreased with approximately 41% (Fig 5B) and that of S. aureus Xen 30 with approximately 50% (Fig 5D), compared to biofilm formation in the absence of nanofibers and copper. Very few cells of P. aeruginosa PA01 and S. aureus Xen 30 adhered to Cu-F (Fig 6B and 6D). Based on these findings, copper that diffused from Cu-F inhibited cell growth and biofilm formation of S. aureus Xen 30 and P. aeruginosa PA01.

Bottom Line: Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter).The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC).After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.

ABSTRACT
Copper particles were incorporated into nanofibers during the electrospinning of poly-D,L-lactide (PDLLA) and poly(ethylene oxide) (PEO). The ability of the nanofibers to prevent Pseudomonas aeruginosa PA01 and Staphylococcus aureus (strain Xen 30) to form biofilms was tested. Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter). The crystalline structure of the copper particles in Cu-F was confirmed by X-ray diffraction (XRD). Copper crystals were encapsulated, but also attached to the surface of Cu-F, as shown scanning transmission electron microscopy (STEM) and transmission electron microscopy (TEM), respectively. The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC). After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively. Reduction in biofilm formation was ascribed to copper released from the nanofibers. Copper-containing nanofibers may be incorporated into wound dressings.

Show MeSH
Related in: MedlinePlus