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Copper-Containing Anti-Biofilm Nanofiber Scaffolds as a Wound Dressing Material.

Ahire JJ, Hattingh M, Neveling DP, Dicks LM - PLoS ONE (2016)

Bottom Line: Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter).The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC).After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.

ABSTRACT
Copper particles were incorporated into nanofibers during the electrospinning of poly-D,L-lactide (PDLLA) and poly(ethylene oxide) (PEO). The ability of the nanofibers to prevent Pseudomonas aeruginosa PA01 and Staphylococcus aureus (strain Xen 30) to form biofilms was tested. Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter). The crystalline structure of the copper particles in Cu-F was confirmed by X-ray diffraction (XRD). Copper crystals were encapsulated, but also attached to the surface of Cu-F, as shown scanning transmission electron microscopy (STEM) and transmission electron microscopy (TEM), respectively. The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC). After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively. Reduction in biofilm formation was ascribed to copper released from the nanofibers. Copper-containing nanofibers may be incorporated into wound dressings.

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X-ray diffraction (XRD) patterns of nanofibers with copper particles (Cu-F), nanofibers without copper particles (CF) and copper particles (Cu).
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pone.0152755.g003: X-ray diffraction (XRD) patterns of nanofibers with copper particles (Cu-F), nanofibers without copper particles (CF) and copper particles (Cu).

Mentions: Diffraction peaks at 2θ values of 43.12°, 50.10°, 74.0°, and 89.6° recorded with XRD corresponded to (111), (200), (220) and (311) crystal planes (Fig 3) of the JCPDS database [37]. These results confirmed the crystalline structure of copper in the nanofibers.


Copper-Containing Anti-Biofilm Nanofiber Scaffolds as a Wound Dressing Material.

Ahire JJ, Hattingh M, Neveling DP, Dicks LM - PLoS ONE (2016)

X-ray diffraction (XRD) patterns of nanofibers with copper particles (Cu-F), nanofibers without copper particles (CF) and copper particles (Cu).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4814046&req=5

pone.0152755.g003: X-ray diffraction (XRD) patterns of nanofibers with copper particles (Cu-F), nanofibers without copper particles (CF) and copper particles (Cu).
Mentions: Diffraction peaks at 2θ values of 43.12°, 50.10°, 74.0°, and 89.6° recorded with XRD corresponded to (111), (200), (220) and (311) crystal planes (Fig 3) of the JCPDS database [37]. These results confirmed the crystalline structure of copper in the nanofibers.

Bottom Line: Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter).The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC).After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.

ABSTRACT
Copper particles were incorporated into nanofibers during the electrospinning of poly-D,L-lactide (PDLLA) and poly(ethylene oxide) (PEO). The ability of the nanofibers to prevent Pseudomonas aeruginosa PA01 and Staphylococcus aureus (strain Xen 30) to form biofilms was tested. Nanofibers containing copper particles (Cu-F) were thinner (326 ± 149 nm in diameter), compared to nanofibers without copper (CF; 445 ± 93 nm in diameter). The crystalline structure of the copper particles in Cu-F was confirmed by X-ray diffraction (XRD). Copper crystals were encapsulated, but also attached to the surface of Cu-F, as shown scanning transmission electron microscopy (STEM) and transmission electron microscopy (TEM), respectively. The copper particles had no effect on the thermal degradation and thermal behaviour of Cu-F, as shown by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC). After 48 h in the presence of Cu-F, biofilm formation by P. aeruginosa PA01 and S. aureus Xen 30 was reduced by 41% and 50%, respectively. Reduction in biofilm formation was ascribed to copper released from the nanofibers. Copper-containing nanofibers may be incorporated into wound dressings.

Show MeSH
Related in: MedlinePlus